TACE抑制劑篩選測定試劑盒(螢光學) | TACE Inhibitor Screening Assay Kit ( Fluorometric)貨號K366

產品介紹: TACE(tumor necrosis factor-α-converting enzyme, 腫瘤壞死因數-β-轉化酶),也稱為ADAM金屬肽酶域17(ADAM metallopeptidase domain 17, ADAM17),是一種70kDa酶,屬於ADAM蛋白系列(ADAM protein family),包括脫黃酶(disintegrins)和金屬蛋白酶(metalloproteases)。TACE被認為參與處理腫瘤壞死因數α(tumor necrosis factor alpha , TNF-α)在細胞表面,並從細胞內膜的跨Golgi network.。這個過程,也稱為” shedding “,涉及從膜結合的親蛋白(如親TNF-+)中分離和釋放可溶性異位,並且具有已知的生理重要性。在 BioVision 的 TACE 抑制劑篩選試劑盒中,TACE 會水解特定的 FRET 基板以釋放淬火螢光組,可在 Ex/Em = 318/449 nm 上以螢光檢測。在強性TACE抑制劑的存在下,基質的水解將受到阻礙。該試劑盒提供快速、簡單、靈敏和可靠的測試,適用於TACE抑制劑的高通量篩選,並可對其進行修改以檢查相關的TACE活性。包括抑制劑控制GM6001用於比較測試抑制劑的療效。

Cat # +Size	K366-100
Size	100 assays
Detection Method	Fluorescence (Ex/Em 318/449 nm)
Species Reactivity	Mammalian
Applications	Provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of TACE inhibitors and can be modified to check the relative TACE activity. Inhibitor control GM6001 is included to compare the efficiency of test inhibitors.
Features & Benefits	• Simple procedure • Fast and convenient • The assay is sensitive, simple, and high-throughput adaptable.
Kit Components	• TACE Assay Buffer • TACE Substrate • TACE Enzyme (20 μg) • Inhibitor Control (0.1 mM GM6001)

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