【MDA脂質過氧化檢測】CheKine™ Lipid Peroxidation (MDA) Assay Kit貨號KTB1050
CheKine™脂質過氧化檢測試劑盒| CheKine™ Lipid Peroxidation (MDA) Assay Kit, MDA貨號KTB1050
氧化應激(Oxidative stress)與包括心血管疾病(chronic diseases)在內的許多慢性疾病的病因有關。 脂質過氧化(Lipid peroxidation)是由於氧化損傷(oxidative damage)而發生的脂質(lipids)降解(degradation),是氧化應激(oxidative stress)的有用標記(useful marker)。 多不飽和脂質(Polyunsaturated lipids)通常易受活性氧(reactive oxygen)的影響而受到氧化攻擊,從而導致產生明確的鏈反應,並生成最終產物,如丙二醛(malondialdehyde ,MDA)脂質過氧化(Lipid peroxidation)可能導致許多疾病的病理,包括動脈粥樣硬化(atherosclerosis),糖尿病(diabetes)和阿爾茨海默氏病(Alzheimer’s)。

CheKine™脂質過氧化(CheKine™ Lipid Peroxidation (MDA) Assay Kit, MDA)檢測試劑盒為檢測各種樣品中存在的丙二醛(malondialdehyde, MDA)提供了便利的工具。 MDA與4-羥基壬烯醛(4-hydroxynonenal , 4-HNE)一起是脂質過氧化(lipid peroxidation)作用的天然副產物,其定量通常用作脂質過氧化(lipid peroxidation)作用的標記。 樣品中的MDA與硫代巴比妥酸(thiobarbituric acid ,TBA)反應生成MDA-TBA加合物,可以很容易地在OD 532 nm處進行比色定量。 同時,測量600nm處的吸光度,並通過532nm和600nm處的吸光度之差計算MDA(以避免蔗糖(saccharose) 的干擾)。1-100 µM/L
Fig. Typical MDA standard calibration curve. The y-axis is ΔOD and the x-axis is MDA concentration (uM).
- Kit components
- Lysis Buffer
• Reaction Mix
• 1 mM Standard - Features & Benefits
- Determination of MDA in serum, plasma, urine, tissue/cell lysates and other biological fluids.
• A broad range linearity: 1-100 µM/L.
• Fast and convenient, measuring lipid peroxidation in a wide range of samples. - Usage notes
- The Reaction Mix may form precipitate, you can sonicate it in a 70°C water bath.
• If not assayed immediately, samples can be stored at -80°C. - Storage instructions
Storage at -20°C immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒
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