TRAP Staining Kit | SERVICEBIO 貨號G1050-50T
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SERVICEBIO系列產品- TRAP Staining Kit
TRAP Staining Kit | SERVICEBIO 貨號G1050-50T
產品介紹:
抗酒石酸酸性磷酸酶（Tartrate resistant acid phosphatase , TRAP）是破骨細胞(osteoclasts) 的標誌酵素。 TRAP 染色試劑盒( TRAP staining kit) 用於觀察組織中的破骨細胞( osteoclasts )。其基本原理是在含有酒石酸的酸性條件下，抗酒石酸磷酸酶TRAP可以水解萘酚AS-BI磷酸酯，生成的萘酚AS-BI與六唑-副玫瑰苯胺結合，形成不溶於水的酒紅色物質沉積在酵素活化的原位位點，實現了抗酒石酸磷酸酶的層析和定位。以本品染色後，破骨細胞的TRAP呈現酒紅色，定位於細胞質。根據切片上每個組織點300 μL的量，該試劑盒可以進行50次以上的TRAP染色。
Assay Protocol
Preparation Before Experiment
Prepare TRAP working fluid:
(1) Take 50 μL pararosaniline solution (G1050-2) and 50 μL sodium nitrite solution (G1050-3) and mix them in a clean centrifuge tube to get hexaazole-pararosaniline solution;
(2) Add 100 μL AS-BI phosphate substrate solution (G1050-4) to 100 μL hexaazole-pararosaniline solution in step 1, and blow and aspirate several times to fully;
(3) Absorb 1.8 mL reaction buffer (G1050-1) and add it to the mixture solution in step 2 mix thoroughly;
(4) The mixed liquid in step 3 is filtered through a needle filter (0.45 μm stream filter film) to obtain the TRAP working liquid.
Note: Be sure to prepare the working solution in the order it belongs to. About 200-300 μL of working solution is required for each tissue point, which should be prepared according to the amount used and used as needed to avoid waste.
Paraffin Sectioning Procedure (For Reference)
1. Paraffin sections are dewaxed to water and washed in pure water for several minutes.
2. Put the sections in a tissue chemical pen circle (with a certain amount of pure water to prevent the drying of sections) in a wet box, and incubate the sections with pure water at 37℃ for 2 h.
3. After the section is incubated, pour out pure water, add filtered TRAP working solution to cover the tissue, and place it at 37 °C in the dark for 20-30 min.
4. (Optional, prepare your own reagents) Counterstain the nuclei: pour the incubation solution and wash it with hematoxylin dye solution to stain the nuclei.
5. Dehydrate, transparent, and seal with neutral gum.
Operation Procedure of Cell Slide (For Reference)
1. Cell fixation: remove the cell culture medium by suction, add 4% paraformaldehyde (G1101 recommended) and fix for 15-30 min, then wash with distilled water for 3 times.
2. Cell membrane rupture: Cells were covered with 0.2% Triton X-100 solution for 20-30 min and gently washed with distilled water for 3 times.
3. Incubation and staining: TRAP working solution was added to the cell well plate to cover the cells, and the cells were incubated at 37℃ for 15-20 min in the dark, and washed with distilled water for 3 times.
4. (Optional, prepare your own reagents) Nuclear counterstaining: Blot the incubation solution and wash it with hematoxylin staining solution for nuclear staining.
5. Add an appropriate amount of absolute ethanol for dehydration, take out the cover glass in the well plate, dry it with a hair dryer, and seal it upside down on the clean glass slide with neutral gum.
| Component Number | Component | G1050-50T |
| G1050-1 | Acetic acid buffer solution & Potassium sodium tartrate | 20 mL |
| G1050-2 | Pararosaniline solution | 1 mL |
| G1050-3 | Sodium nitrite solution | 1 mL |
| G1050-4 | AS-BI Phosphate Solution | 1 mL |
|
Cat.No.
|
Product Name
|
Spec.
|
|---|
|
G1011-10ML
|
Hoechst 33258 Staining Solution (Ready-To-Use)
Hoechst 33258 染色液（即用型） |
10 mL
|
|
G1012-100ML
|
DAPI Staining Reagent (Ready to Use)
DAPI 染色試劑（即使用型） |
100 mL
|
|
G1012-10ML
|
DAPI Staining Reagent (Ready to Use)
DAPI 染色試劑（即使用型） |
10 mL
|
|
G1021-10ML
|
PI Stain
碘化丙啶(propidium iodide, PI) |
10 mL
|
|
G1127-1ML
|
Hoechst 33342 Staining Solution
Hoechst 33342 染色液 |
1 mL
|
|
G1515-100T
|
JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 粒線體膜電位檢測試劑盒 |
100 T
|
|
G1704
|
DiO Cell Membrane Green Fluorescence Staining Kit
DiO 細胞膜綠色螢光染色試劑盒 |
100-1000 T
|
|
G1705
|
Dil Cell Membrane Red Fluorescence Staining Kit
Dil 細胞膜紅色螢光染色試劑盒 |
100-1000 T
|
|
G1720-20UL
|
ER-Tracker Green
ER-追蹤器綠色(ER-Tracker Green) |
20 μL
|
|
G1721-20UL
|
ER-Tracker Red
ER-追蹤器紅色(ER-Tracker Red) |
20 μL
|
|
G1722-50UL
|
Lyso-Tracker Green
溶血追蹤器綠色(Lyso-Tracker Green) |
50 μL
|
|
G1723-50UG
|
MitoTracker Red CMXRos
MitoTracker 紅色 |
50 μg
|
|
G1905-100T
|
Lipid Droplets Fluorescence Assay Kit
脂滴螢光檢測試劑盒 |
100 T
|
Servicebio台灣品牌介紹-BRAND/AGENCY/品牌介紹- Servicebio
| Classfication | Cat No. | Name | Place of Origin | Spec. |
|
Amino Acids
|
GC304002 | L-Glutamine | 產品資料 | 100 g |
| GC304003 | L-Arginine hydrochloride | 產品資料 | 25 g | |
| GC304004-100g | L-Glutamine acid | 產品資料 | 100 g | |
| GC304005 | L-Isoleucine | 產品資料 | 25 g | |
| GC304006-25g | L-Leucine | 產品資料 | 25 g | |
| GC304007 | L-Lysine hydrochloride | 產品資料 | 25 g | |
| GC304009 | L-Methionine | 產品資料 | 25 g | |
| GC304010 | L-Phenylalanine | 產品資料 | 10 g | |
| GC304011-25g | L-Alanine | 產品資料 | 25 g | |
| GC304012 | L-Histidine | 產品資料 | 25 g | |
| GC304013 | L-Serine | 產品資料 | 25 g | |
| GC304014 | L-tryptophan | 產品資料 | 5 g | |
| GC304016 | L-Threonine | 產品資料 | 25 g | |
| GC304017 | L-Valine | 產品資料 | 10 g | |
| GC304019 | Glycine | 產品資料 | 500 g | |
| GC304021 | L-Histidine hydrochloride monohydrate | 產品資料 | 25 g | |
| GC304022 | Glycine cell culture grade 甘氨酸Glycine (細胞培養等級 |
產品資料 | 100 g | |
|
Protein Derivatives
|
GC305002-5g | Iodoacetamide | 產品資料 | 5 g |
| GC305003 | Trypsin 1:250 Source: Porcine Pancreas | 產品資料 | 10 g | |
| GC305004-5g | Pepsin 1:3000 | 產品資料 | 5 g | |
| GC305005 | Horseradish peroxidase | 產品資料 | 1 g | |
| GC305006 | Bovine Serum Albumin – import | 產品資料 | 100 g | |
| GC305009 | Human Albumin-FITC | 產品資料 | 0.5 mL | |
| GC305010 | Bovine Serum Albumin BSA | 產品資料 | 100 g | |
| GC305010-25G | Bovine Serum Albumin BSA | 產品資料 | 25 g | |
| GC305010-50G | Bovine Serum Albumin BSA | 產品資料 | 50 g | |
| GC305010-5G | Bovine Serum Albumin BSA | 產品資料 | 5 g | |
| GC305013-100mg | Collagenase I | 產品資料 | 100 mg | |
| GC305014-100mg | Collagenase II | 產品資料 | 100 mg | |
| GC305015-100mg | Collagenase IV 膠原酶IV (Collagenase IV) |
產品資料 | 100 mg | |
| GC305016-100g | Gelatine | 產品資料 | 100 g | |
|
Hormones
|
GC302002-25mg | Abscisic acid | 產品資料 | 25 mg |
| GC302003 | Angiotensin II | 產品資料 | 10 mg | |
|
Biological pigments
|
GC307002-25g | Crystal violet | 產品資料 | 25 g |
| GC307003-5g | Trypan Blue | 產品資料 | 5 g | |
| GC307005 | Alcian Blue8GX | 產品資料 | 1 g | |
| GC307006-5g | Bromphenol Blue | 產品資料 | 5 g | |
| GC307007 | Serva blue G-250 | 產品資料 | 10 g | |
| GC307008-5g | Congo Red | 產品資料 | 5 g | |
| GC307009-25g | Alizarin Red S | 產品資料 | 25 g | |
| GC307010-1g | Giemsa′s Stain | 產品資料 | 1 g | |
| GC307011 | Fuchsin basic | 產品資料 | 5 g | |
| GC307012-10g | Ponceau S | 產品資料 | 10 g | |
| GC307013 | Acid red 26 2R | 產品資料 | 5 g | |
| GC307014 | Direct Red 80 | 產品資料 | 5 g | |
| GC307015-5g | Sudan Black B | 產品資料 | 5 g | |
| GC307017 | Aniline blue | 產品資料 | 5 g | |
| GC307018 | Nuclear fast red | 產品資料 | 5 g | |
| GC307019 | Solvent Blue 38 | 產品資料 | 5 g | |
| GC307020 | Hematoxylin | 產品資料 | 5 g | |
| GC307021 | Solvent Red 43 | 產品資料 | 10 g | |
| GC307022 | Eosin Y, water soluble | 產品資料 | 10 g | |
| GC307023 | Safranin O | 產品資料 | 10 g | |
| GC307024 | Fast Green | 產品資料 | 10 g | |
| GC307026-5g | Pyronin G | 產品資料 | 5 g | |
| GC307027-1g | Janus Green B | 產品資料 | 1 g | |
| GC307030-10g | Brilliant Cresyl Blue | 產品資料 | 10 g | |
| GC307031 | Coomassie brilliant blue R-250 | 產品資料 | 5 g | |
| GC307034-1g | Thionine acetate | 產品資料 | 1 g | |
| GC307035-1g | Cresyl Fast Violet | 產品資料 | 1 g | |
| GC307036-1g | Orcein | 產品資料 | 1 g | |
| GC307037-1g | Carmine | 產品資料 | 1 g | |
| GC307039-10g | Mordant Blue 29 | 產品資料 | 10 g | |
| GC307042-5g | Oil Red O | 產品資料 | 5 g | |
| GC307045-25g | Rhodamine B | 產品資料 | 25 g | |
|
Vitamins
|
GC301015-25g | Vitamin C | 產品資料 | 25 g |
| GC303001 | Folic acid | 產品資料 | 5 g | |
| GC303004 | Ribofavin | 產品資料 | 25 g | |
| GC303005-5g | VitaminB6 | 產品資料 | 5 g | |
| GC303006-25g | VitaminB3 | 產品資料 | 25 g | |
| GC303008 | VitaminB12 | 產品資料 | 1 g | |
| GC303011-25mg | NADPH-Na4 | 產品資料 | 25 mg | |
| GC303013-250mg | β-nicotinamide adenine dinucleotide NAD | 產品資料 | 250 mg | |
| GC303014-250mg | NADH-Na2 | 產品資料 | 250 mg | |
|
Antibiotics
|
GC301002 | Streptozocin STZ | 產品資料 | 1 g |
| GC301002-100mg | Streptozocin STZ | 產品資料 | 100 mg | |
| GC301003 | Penicillin G Sodium salt | 產品資料 | 1 g | |
| GC301004 | Ampicillin | 產品資料 | 1 g | |
| GC301005 | Streptomycin sulfate | 產品資料 | 5 g | |
| GC301008 | Kanamycin | 產品資料 | 1 g | |
| GC301009 | Kanamycin sulfate | 產品資料 | 5 g | |
| GC301009-1g | Hygromycin B | 產品資料 | 1 g | |
| GC301011 | Geneticin G-418 | 產品資料 | 1 g | |
| GC301013 | Chloramphenicol | 產品資料 | 5 g | |
| GC301013-10mg | Pseudomonic acid | 產品資料 | 10 mg | |
| GC301014-5g | Norfloxacin | 產品資料 | 5 g | |
| GC301016 | Cisplatin | 產品資料 | 100 mg | |
| GC301017-100mg | Sirolimus | 產品資料 | 100 mg | |
|
Heterocyclic compounds
|
GC207001 | Imidazole | 產品資料 | 10 g |
| GC207002 | PMSF | 產品資料 | 5 g | |
| GC207003 | Freund’s Adjuvant Complete | 產品資料 | 10 mL | |
| GC207004 | Freund’s Adjuvant Incomplete | 產品資料 | 10 mL | |
|
Salt
|
GC201001 | Guanidine HCl | 產品資料 | 500 g |
| GC201002 | Guanidine thiocyanate | 產品資料 | 500 g | |
|
Sugar Alcohols
|
GC205001 | L(+)-Lactic acid | 產品資料 | 100 g |
| GC205002 | Pyruvic acid sodium salt | 產品資料 | 25 g | |
| GC205003 | D-Glucose, anhydrous | 產品資料 | 500 g | |
| GC205004-10g | D(+)-Trehalose dihydrate | 產品資料 | 10 g | |
| GC205005 | D(+)- Galactose | 產品資料 | 100 g | |
| GC205006-10g | D-(-)Arabinose | 產品資料 | 10 g | |
| GC205007-1g | DTE | 產品資料 | 1 g | |
| GC205008 | Glycerol | 產品資料 | 100 mL | |
| GC205009 | LPS | 產品資料 | 10 mg | |
| GC205010 | DTT | 產品資料 | 1 g | |
| GC205011 | IPTG | 產品資料 | 1 g | |
| GC205013 | Agarose | 產品資料 | 100 g | |
| GC205014 | Sucrose | 產品資料 | 500 g | |
| GC205015 | D-Sorbitol | 產品資料 | 500 g | |
|
Solvent
|
GC203001 | TEMED | 產品資料 | 100 mL |
| GC203002 | DMSO(Dimethyl sulfoxide) | 產品資料 | 100 mL | |
| GC203003 | Urea | 產品資料 | 500 g | |
| GC203005 | DMSO cellular level | 產品資料 | 10 mL | |
|
Complex
|
GC202001 | EDTA | 產品資料 | 500 g |
| GC202002 | EDTA -Na2 | 產品資料 | 500 g | |
|
Buffer salt
|
GC208001 | Tris (Hydroxymethyl) Aminomethane | 產品資料 | 500 g |
| GC208003 | RIS-HCL | 產品資料 | 500 g | |
| GC208004-100g | BIS-TRIS | 產品資料 | 100 g | |
| GC208006 | N-Tris[Hydroxymethyl]Methylglycine | 產品資料 | 100 g | |
| GC208007 | 4-propanesulfonyl morpholine | 產品資料 | 100 g | |
| GC208008-25g | HEPPS | 產品資料 | 25 g | |
| GC208009 | HEPES, Free acid | 產品資料 | 100 g | |
| GC208012-1g | TCEP·HCL | 產品資料 | 1 g | |
|
Surfactant
|
GC204001 | Tween-80 | 產品資料 | 100 mL |
| GC204002 | Tween-20 | 產品資料 | 100 mL | |
| GC204003 | Triton X-100 | 產品資料 | 100 mL | |
| GC204004 | Sodium dodecylsulfate | 產品資料 | 500 g | |
| GC204005 | Sodium dodecylsulfate | 產品資料 | 100 g | |
| GC204006 | Nonidet TM P40 Substitute NP-40 | 產品資料 | 100 g | |
| GC204007 | Deoxycholic acid， sodium salt | 產品資料 | 25 g | |
| GC204009-100ml | APES | 產品資料 | 100 mL | |
|
Amine organics
|
GC206002 | Acrylamide | 產品資料 | 500 g |
| GC206003 | N’-MBA | 產品資料 | 100 g | |
|
Polymer
|
GC209002-250g | PEG 8000 | 產品資料 | 250 g |
| GC209003-250g | PEG 6000 | 產品資料 | 250 g | |
| GC209004-100g | PEG 3350 | 產品資料 | 100 g | |
| GC209005-500g | Poly(ethylene glycol)2000 | 產品資料 | 500 g | |
| GC209006-250g | Poly(ethylene glycol) 20000 | 產品資料 | 250 g | |
|
Inorganic salt
|
GC106001 | Orthoboric acid | 產品資料 | 500 g |
| GC104004 | Sodium bicarbonate | 產品資料 | 500 g | |
| GC108003 | Citric acid monohydrate | 產品資料 | 100 g | |
| GC108004 | Sodium citrate dihydrate | 產品資料 | 100 g | |
| GC102001 | Calcium chloride | 產品資料 | 100 g | |
| GC102002 | Magnesium chloride hexahydrate | 產品資料 | 100 g | |
| GC102003 | Potassium chloride | 產品資料 | 500 g | |
| GC102004 | Sodium chloride | 產品資料 | 500 g | |
| GC101004 | Magnesium Sulfate | 產品資料 | 100 g | |
| GC101013 | Ammonium persulfate | 產品資料 | 25 g | |
| GC105001 | Sodium phosphate monobasic | 產品資料 | 500 g | |
| GC105002 | Sodium Phosphate, Dibasic | 產品資料 | 500 g | |
| GC105003 | Potassium Phosphate Monobasic | 產品資料 | 500 g | |
| GC107001 | Sodium hydroxide | 產品資料 | 100 g | |
| GC306001 | Lodixanol | 產品資料 | 5 g | |
| GC310001-100g | skimmed milk powder | 產品資料 | 100 g |