MitoTracker 紅色 CMXRos(MitoTracker Red CMXRos) | SERVICEBIO 貨號G1723-50UG
SERVICEBIO細胞螢光染色(Cell Fluorescence Staining)系列產品- CMXRos (MitoTracker Red CMXRos)
MitoTracker 紅色 CMXRos(MitoTracker Red CMXRos) | SERVICEBIO 貨號G1723-50UG
產品介紹:
MitoTracker Red CMXRos（粒線體紅色螢光探針）是一種 X-玫瑰胺衍生物(X-rosamine derivative)，分子量為 531.52，激發波長為 578 ± 3 nm，發射波長為 599 ± 4 nm。該染料可以直接進入活細胞並定位在粒線體(mitochondria)上，並能夠與粒線體(mitochondria)共價結合。粒線體特異性標記的原理是帶正電荷的探針骨架(skeleton)可以定位在帶負電荷( positive charge )的粒線體上，探針上的氯甲基( chloromethyl group)可以與粒線體中游離的巰基( free sulfhydryl group)共價結合，起到螢光標記的作用;只需將細胞線粒體與活細胞一起孵育一定時間即可進行標記，標記線粒體(mitochondria)後，可透過固定、穿孔對線粒體進行進一步處理，可用於後續的多重標記實驗。
| Component | G1723-50UG |
| Mito Tracker Red CMXRos | 50 μg |
| Manual | 1 pc |
Assay Protocol
1. Assay working solution preparation:
1.1. This product is in the form of a dry powder, which should be centrifuged briefly at high speed before use to ensure that it settles to the bottom of the tube. Provide your own cell grade DMSO.
1.2. Preparation of storage solution: Add 94 μL DMSO (cell level) into the tube, blow and mix well to fully dissolve the dye, that is to say, the mitochondrial fluorescent dye storage solution with a concentration of 1 mM (molecular weight information on the product label) is obtained, and then centrifuge briefly at a high speed to avoid the loss of reagents hanging on the wall. Store the storage solution at -20°C. It is recommended to store it in smaller sizes and avoid repeated freezing and thawing.
1.3. Preparation of assay working solution: If the assay is performed directly after staining, dilute the storage solution into mitochondrial assay working solution at a final concentration of 0.1-0.25 μM with biological buffer (Hanks, PBS) or serum-free basal medium; If subsequent operations such as fixation and perforation are required after staining, dilute the storage solution into mitochondrial assay working solution at a final concentration of 0.4-0.6 μM with biological buffer (Hanks, PBS) or serum-free basal medium; if the assay is performed after staining, dilute the storage solution into mitochondrial assay working solution at a final concentration of 0.4-0.6 μM with biological buffer (Hanks, PBS) or serum-free basal medium.
2. Cell staining (the following protocol is for adherent cells; centrifugation is required for suspension cell exchange)
2.1. The cells are planted on cell culture plates or cell climbing plates until they converge to a certain extent;
2.2. The cell culture medium is removed and washed 1-2 times with preheated buffer for 3-5 min each time;
2.3. Add the preheated working solution for mitochondrial detection and incubate at 37°C for 30 min in a cell culture incubator ( due to different cell types and states, the incubation time can be adjusted to some extent);
2.4. Remove the incubation solution and wash with buffer solution 2-3 times for 3-5 min each time;
2.5. Directly or with anti-fluorescence quenching sealing solution (recommended G1401) the films are sealed and observed under a fluorescence microscope (Ex=579 nm, Em=599 nm);
3. Cell fixation and perforation and other manipulations (optional):
Note: After staining, this product can be fixed and perforated, but the fluorescent signal will be attenuated after fixation.
3.1. Cell fixation
3.1.1. Cells treated with mitochondrial fluorescent dye labeling staining and washing are added to fixative (recommended G1101) and fixed for 10 min at room temperature;
3.1.2. Remove the fixative and wash with buffer 2-3 times for 3-5 min each time;
3.2. Cell perforation
3.2.1. The fixed and washed cells are added with 0.2-0.5% Triton X-100 and perforated for 10 min at room temperature;
3.2.2. The perforating fluid is removed and washed with buffer solution 2-3 times for 3-5 min each time;
3.3. The cells are stained and then fixed and perforated for processing, and can be used for the next experiments such as multilabeling.
Fluorescence Microscopy to Observe The Procedure of Living Dead Cells：
1. Due to the different types and states of the cells, the working concentration of the dye and the incubation time can be adjusted appropriately.
2. The dye needs to be used for mitochondrial localization in the state of living cells, and the cells cannot be fixed first, otherwise it will lead to false positive results of inaccurate localization.Fixation and subsequent operations can be performed after staining of living cells.
3. When staining and washing live cells, the assay working solution and washing buffer need to be pre-warmed at 37°C. Staining and washing are accomplished by incubation in an incubator so as not to affect the morphology of the cells by temperature difference stimulation.
4. In order to prevent fluorescence quenching, the entire staining process needs to be operated away from light.
5. Probe master mixes should be stored in separate containers to avoid repeated freezing and thawing.To avoid waste, the staining solution is prepared as needed.
6. For your health and safety, please wear lab coat and gloves during operation.
流式細胞儀偵測細胞週期程序：
1. 測定工作液的配製：
1.1.本品為乾粉狀，使用前應高速短暫離心，以確保其沉澱至管底。提供您自己的細胞級 DMSO。
1.2.儲存液的配製：在管中加入94 μL DMSO（細胞水平），吹勻，使染料充分溶解，即得到濃度為1 mM的粒線體螢光染料儲存液（分子量資訊在管中）以獲得產品標籤），然後高速短暫離心，以避免掛在牆上的試劑損失。將儲存溶液儲存於-20°C。建議較小尺寸保存，避免反覆凍融。
1.3.測定工作液的配製：若染色後直接進行測定，則以生物緩衝液（Hanks、PBS）或無血清基礎培養基將儲存液稀釋成粒線體測定工作液，終濃度為0.1-0.25 μM；染色後如需進行固定、穿孔等後續操作，可用生物緩衝液（Hanks、PBS）或無血清基礎培養基將保存液稀釋成粒線體測定工作液，終濃度為0.4-0.6 μM；若染色後進行測定，則以生物緩衝液（Hanks、PBS）或無血清基礎培養基將儲存液稀釋成粒線體測定工作液，終濃度為0.4-0.6 μM。
2. 細胞染色（以下方案適用於貼壁細胞；懸浮細胞交換需離心）
2.1.將細胞種植在細胞培養板或細胞攀爬板上，直到聚集到一定程度；
2.2.移除細胞培養基，以預熱緩衝液洗滌1-2次，每小時3-5分鐘；
2.3.加入預熱的粒線體檢測工作液，在細胞培養箱中37℃孵育30 min（因細胞型和狀態不同，孵育時間可調整）；
2.4.除去孵育液，用緩衝液洗滌2-3次，每次3-5分鐘；
2.5.直接或以抗螢光淬滅封膜液（建議G1401）封膜，在螢光顯微鏡下觀察（Ex=579 nm，Em=599 nm）；
3. 細胞固定和穿孔等操作（可選）：
注意：本品染色後可固定打孔，但固定後螢光訊號會減弱。
3.1.細胞固定
3.1.1.經粒線體螢光染料標記染色、洗滌後的細胞加入固定液（建議G1101），室溫固定10min；
3.1.2.除去固定液，用緩衝液洗滌2-3次，每次3-5分鐘；
3.2.細胞穿孔
3.2.1.固定、洗滌的細胞加入0.2-0.5% Triton X-100，室溫穿孔10 min；
3.2.2.除去射孔液，用緩衝液沖洗2-3次，每小時3-5分鐘；
3.3.將細胞染色，然後固定並穿孔進行處理，可用於下一步的實驗，例如多重標記。
螢光顯微鏡觀察活死細胞的過程：
1. 由於細胞類型和狀態不同，染料的工作濃度和培養時間可適當調整。
2. 染料需要在活細胞狀態下進行粒線體定位，且無法先固定細胞，否則會導致定位不準確的假陽性結果，活細胞染色後即可進行固定及後續操作。
3. 活細胞染色和洗滌時，檢測工作液和洗滌緩衝液需預熱至37°C。染色和洗滌是透過在培養箱中孵育來完成，以免溫差刺激影響細胞的形態。
4.為了防止螢光猝滅，整個染色過程需要避光操作。
5. 探針預混液應單獨存放，避免反覆凍融，染色液按需配製，避免浪費。
6.為了您的健康與安全，操作時請穿戴實驗服並戴上手套。
|
Cat.No.
|
Product Name
|
Spec.
|
|---|
|
G1011-10ML
|
Hoechst 33258 Staining Solution (Ready-To-Use)
Hoechst 33258 染色液（即用型） |
10 mL
|
|
G1012-100ML
|
DAPI Staining Reagent (Ready to Use)
DAPI 染色試劑（即使用型） |
100 mL
|
|
G1012-10ML
|
DAPI Staining Reagent (Ready to Use)
DAPI 染色試劑（即使用型） |
10 mL
|
|
G1021-10ML
|
PI Stain
碘化丙啶(propidium iodide, PI) |
10 mL
|
|
G1127-1ML
|
Hoechst 33342 Staining Solution
Hoechst 33342 染色液 |
1 mL
|
|
G1515-100T
|
JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 粒線體膜電位檢測試劑盒 |
100 T
|
|
G1704
|
DiO Cell Membrane Green Fluorescence Staining Kit
DiO 細胞膜綠色螢光染色試劑盒 |
100-1000 T
|
|
G1705
|
Dil Cell Membrane Red Fluorescence Staining Kit
Dil 細胞膜紅色螢光染色試劑盒 |
100-1000 T
|
|
G1720-20UL
|
ER-Tracker Green
ER-追蹤器綠色(ER-Tracker Green) |
20 μL
|
|
G1721-20UL
|
ER-Tracker Red
ER-追蹤器紅色(ER-Tracker Red) |
20 μL
|
|
G1722-50UL
|
Lyso-Tracker Green
溶血追蹤器綠色(Lyso-Tracker Green) |
50 μL
|
|
G1723-50UG
|
MitoTracker Red CMXRos
MitoTracker 紅色 |
50 μg
|
|
G1905-100T
|
Lipid Droplets Fluorescence Assay Kit
脂滴螢光檢測試劑盒 |
100 T
|
Servicebio台灣品牌介紹-BRAND/AGENCY/品牌介紹- Servicebio
| Classfication | Cat No. | Name | Place of Origin | Spec. |
|
Amino Acids
|
GC304002 | L-Glutamine | 產品資料 | 100 g |
| GC304003 | L-Arginine hydrochloride | 產品資料 | 25 g | |
| GC304004-100g | L-Glutamine acid | 產品資料 | 100 g | |
| GC304005 | L-Isoleucine | 產品資料 | 25 g | |
| GC304006-25g | L-Leucine | 產品資料 | 25 g | |
| GC304007 | L-Lysine hydrochloride | 產品資料 | 25 g | |
| GC304009 | L-Methionine | 產品資料 | 25 g | |
| GC304010 | L-Phenylalanine | 產品資料 | 10 g | |
| GC304011-25g | L-Alanine | 產品資料 | 25 g | |
| GC304012 | L-Histidine | 產品資料 | 25 g | |
| GC304013 | L-Serine | 產品資料 | 25 g | |
| GC304014 | L-tryptophan | 產品資料 | 5 g | |
| GC304016 | L-Threonine | 產品資料 | 25 g | |
| GC304017 | L-Valine | 產品資料 | 10 g | |
| GC304019 | Glycine | 產品資料 | 500 g | |
| GC304021 | L-Histidine hydrochloride monohydrate | 產品資料 | 25 g | |
| GC304022 | Glycine cell culture grade 甘氨酸Glycine (細胞培養等級 |
產品資料 | 100 g | |
|
Protein Derivatives
|
GC305002-5g | Iodoacetamide | 產品資料 | 5 g |
| GC305003 | Trypsin 1:250 Source: Porcine Pancreas | 產品資料 | 10 g | |
| GC305004-5g | Pepsin 1:3000 | 產品資料 | 5 g | |
| GC305005 | Horseradish peroxidase | 產品資料 | 1 g | |
| GC305006 | Bovine Serum Albumin – import | 產品資料 | 100 g | |
| GC305009 | Human Albumin-FITC | 產品資料 | 0.5 mL | |
| GC305010 | Bovine Serum Albumin BSA | 產品資料 | 100 g | |
| GC305010-25G | Bovine Serum Albumin BSA | 產品資料 | 25 g | |
| GC305010-50G | Bovine Serum Albumin BSA | 產品資料 | 50 g | |
| GC305010-5G | Bovine Serum Albumin BSA | 產品資料 | 5 g | |
| GC305013-100mg | Collagenase I | 產品資料 | 100 mg | |
| GC305014-100mg | Collagenase II | 產品資料 | 100 mg | |
| GC305015-100mg | Collagenase IV 膠原酶IV (Collagenase IV) |
產品資料 | 100 mg | |
| GC305016-100g | Gelatine | 產品資料 | 100 g | |
|
Hormones
|
GC302002-25mg | Abscisic acid | 產品資料 | 25 mg |
| GC302003 | Angiotensin II | 產品資料 | 10 mg | |
|
Biological pigments
|
GC307002-25g | Crystal violet | 產品資料 | 25 g |
| GC307003-5g | Trypan Blue | 產品資料 | 5 g | |
| GC307005 | Alcian Blue8GX | 產品資料 | 1 g | |
| GC307006-5g | Bromphenol Blue | 產品資料 | 5 g | |
| GC307007 | Serva blue G-250 | 產品資料 | 10 g | |
| GC307008-5g | Congo Red | 產品資料 | 5 g | |
| GC307009-25g | Alizarin Red S | 產品資料 | 25 g | |
| GC307010-1g | Giemsa′s Stain | 產品資料 | 1 g | |
| GC307011 | Fuchsin basic | 產品資料 | 5 g | |
| GC307012-10g | Ponceau S | 產品資料 | 10 g | |
| GC307013 | Acid red 26 2R | 產品資料 | 5 g | |
| GC307014 | Direct Red 80 | 產品資料 | 5 g | |
| GC307015-5g | Sudan Black B | 產品資料 | 5 g | |
| GC307017 | Aniline blue | 產品資料 | 5 g | |
| GC307018 | Nuclear fast red | 產品資料 | 5 g | |
| GC307019 | Solvent Blue 38 | 產品資料 | 5 g | |
| GC307020 | Hematoxylin | 產品資料 | 5 g | |
| GC307021 | Solvent Red 43 | 產品資料 | 10 g | |
| GC307022 | Eosin Y, water soluble | 產品資料 | 10 g | |
| GC307023 | Safranin O | 產品資料 | 10 g | |
| GC307024 | Fast Green | 產品資料 | 10 g | |
| GC307026-5g | Pyronin G | 產品資料 | 5 g | |
| GC307027-1g | Janus Green B | 產品資料 | 1 g | |
| GC307030-10g | Brilliant Cresyl Blue | 產品資料 | 10 g | |
| GC307031 | Coomassie brilliant blue R-250 | 產品資料 | 5 g | |
| GC307034-1g | Thionine acetate | 產品資料 | 1 g | |
| GC307035-1g | Cresyl Fast Violet | 產品資料 | 1 g | |
| GC307036-1g | Orcein | 產品資料 | 1 g | |
| GC307037-1g | Carmine | 產品資料 | 1 g | |
| GC307039-10g | Mordant Blue 29 | 產品資料 | 10 g | |
| GC307042-5g | Oil Red O | 產品資料 | 5 g | |
| GC307045-25g | Rhodamine B | 產品資料 | 25 g | |
|
Vitamins
|
GC301015-25g | Vitamin C | 產品資料 | 25 g |
| GC303001 | Folic acid | 產品資料 | 5 g | |
| GC303004 | Ribofavin | 產品資料 | 25 g | |
| GC303005-5g | VitaminB6 | 產品資料 | 5 g | |
| GC303006-25g | VitaminB3 | 產品資料 | 25 g | |
| GC303008 | VitaminB12 | 產品資料 | 1 g | |
| GC303011-25mg | NADPH-Na4 | 產品資料 | 25 mg | |
| GC303013-250mg | β-nicotinamide adenine dinucleotide NAD | 產品資料 | 250 mg | |
| GC303014-250mg | NADH-Na2 | 產品資料 | 250 mg | |
|
Antibiotics
|
GC301002 | Streptozocin STZ | 產品資料 | 1 g |
| GC301002-100mg | Streptozocin STZ | 產品資料 | 100 mg | |
| GC301003 | Penicillin G Sodium salt | 產品資料 | 1 g | |
| GC301004 | Ampicillin | 產品資料 | 1 g | |
| GC301005 | Streptomycin sulfate | 產品資料 | 5 g | |
| GC301008 | Kanamycin | 產品資料 | 1 g | |
| GC301009 | Kanamycin sulfate | 產品資料 | 5 g | |
| GC301009-1g | Hygromycin B | 產品資料 | 1 g | |
| GC301011 | Geneticin G-418 | 產品資料 | 1 g | |
| GC301013 | Chloramphenicol | 產品資料 | 5 g | |
| GC301013-10mg | Pseudomonic acid | 產品資料 | 10 mg | |
| GC301014-5g | Norfloxacin | 產品資料 | 5 g | |
| GC301016 | Cisplatin | 產品資料 | 100 mg | |
| GC301017-100mg | Sirolimus | 產品資料 | 100 mg | |
|
Heterocyclic compounds
|
GC207001 | Imidazole | 產品資料 | 10 g |
| GC207002 | PMSF | 產品資料 | 5 g | |
| GC207003 | Freund’s Adjuvant Complete | 產品資料 | 10 mL | |
| GC207004 | Freund’s Adjuvant Incomplete | 產品資料 | 10 mL | |
|
Salt
|
GC201001 | Guanidine HCl | 產品資料 | 500 g |
| GC201002 | Guanidine thiocyanate | 產品資料 | 500 g | |
|
Sugar Alcohols
|
GC205001 | L(+)-Lactic acid | 產品資料 | 100 g |
| GC205002 | Pyruvic acid sodium salt | 產品資料 | 25 g | |
| GC205003 | D-Glucose, anhydrous | 產品資料 | 500 g | |
| GC205004-10g | D(+)-Trehalose dihydrate | 產品資料 | 10 g | |
| GC205005 | D(+)- Galactose | 產品資料 | 100 g | |
| GC205006-10g | D-(-)Arabinose | 產品資料 | 10 g | |
| GC205007-1g | DTE | 產品資料 | 1 g | |
| GC205008 | Glycerol | 產品資料 | 100 mL | |
| GC205009 | LPS | 產品資料 | 10 mg | |
| GC205010 | DTT | 產品資料 | 1 g | |
| GC205011 | IPTG | 產品資料 | 1 g | |
| GC205013 | Agarose | 產品資料 | 100 g | |
| GC205014 | Sucrose | 產品資料 | 500 g | |
| GC205015 | D-Sorbitol | 產品資料 | 500 g | |
|
Solvent
|
GC203001 | TEMED | 產品資料 | 100 mL |
| GC203002 | DMSO(Dimethyl sulfoxide) | 產品資料 | 100 mL | |
| GC203003 | Urea | 產品資料 | 500 g | |
| GC203005 | DMSO cellular level | 產品資料 | 10 mL | |
|
Complex
|
GC202001 | EDTA | 產品資料 | 500 g |
| GC202002 | EDTA -Na2 | 產品資料 | 500 g | |
|
Buffer salt
|
GC208001 | Tris (Hydroxymethyl) Aminomethane | 產品資料 | 500 g |
| GC208003 | RIS-HCL | 產品資料 | 500 g | |
| GC208004-100g | BIS-TRIS | 產品資料 | 100 g | |
| GC208006 | N-Tris[Hydroxymethyl]Methylglycine | 產品資料 | 100 g | |
| GC208007 | 4-propanesulfonyl morpholine | 產品資料 | 100 g | |
| GC208008-25g | HEPPS | 產品資料 | 25 g | |
| GC208009 | HEPES, Free acid | 產品資料 | 100 g | |
| GC208012-1g | TCEP·HCL | 產品資料 | 1 g | |
|
Surfactant
|
GC204001 | Tween-80 | 產品資料 | 100 mL |
| GC204002 | Tween-20 | 產品資料 | 100 mL | |
| GC204003 | Triton X-100 | 產品資料 | 100 mL | |
| GC204004 | Sodium dodecylsulfate | 產品資料 | 500 g | |
| GC204005 | Sodium dodecylsulfate | 產品資料 | 100 g | |
| GC204006 | Nonidet TM P40 Substitute NP-40 | 產品資料 | 100 g | |
| GC204007 | Deoxycholic acid， sodium salt | 產品資料 | 25 g | |
| GC204009-100ml | APES | 產品資料 | 100 mL | |
|
Amine organics
|
GC206002 | Acrylamide | 產品資料 | 500 g |
| GC206003 | N’-MBA | 產品資料 | 100 g | |
|
Polymer
|
GC209002-250g | PEG 8000 | 產品資料 | 250 g |
| GC209003-250g | PEG 6000 | 產品資料 | 250 g | |
| GC209004-100g | PEG 3350 | 產品資料 | 100 g | |
| GC209005-500g | Poly(ethylene glycol)2000 | 產品資料 | 500 g | |
| GC209006-250g | Poly(ethylene glycol) 20000 | 產品資料 | 250 g | |
|
Inorganic salt
|
GC106001 | Orthoboric acid | 產品資料 | 500 g |
| GC104004 | Sodium bicarbonate | 產品資料 | 500 g | |
| GC108003 | Citric acid monohydrate | 產品資料 | 100 g | |
| GC108004 | Sodium citrate dihydrate | 產品資料 | 100 g | |
| GC102001 | Calcium chloride | 產品資料 | 100 g | |
| GC102002 | Magnesium chloride hexahydrate | 產品資料 | 100 g | |
| GC102003 | Potassium chloride | 產品資料 | 500 g | |
| GC102004 | Sodium chloride | 產品資料 | 500 g | |
| GC101004 | Magnesium Sulfate | 產品資料 | 100 g | |
| GC101013 | Ammonium persulfate | 產品資料 | 25 g | |
| GC105001 | Sodium phosphate monobasic | 產品資料 | 500 g | |
| GC105002 | Sodium Phosphate, Dibasic | 產品資料 | 500 g | |
| GC105003 | Potassium Phosphate Monobasic | 產品資料 | 500 g | |
| GC107001 | Sodium hydroxide | 產品資料 | 100 g | |
| GC306001 | Lodixanol | 產品資料 | 5 g | |
| GC310001-100g | skimmed milk powder | 產品資料 | 100 g |