DiO 細胞膜綠色螢光染色試劑盒(DiO Cell Membrane Green Fluorescence Staining Kit) | SERVICEBIO 貨號G1704
SERVICEBIO細胞螢光染色(Cell Fluorescence Staining)系列產品-DiO 細胞膜染劑
DiO 細胞膜綠色螢光染色試劑盒(DiO Cell Membrane Green Fluorescence Staining Kit) | SERVICEBIO 貨號G1704
產品介紹:
DiO，又稱DiOC18(3)，3-十八烷基-2-[3-(3-十八烷基-2(3H)-苯並噁唑-2-亞基)-1-丙烯-1 -基]苯並噁唑高氯酸鹽(3-octadecyl-2 -[3-(3-octadecyl-2 (3H) -benzooxazole-2-subunit) -1-propene-1-yl] benzooxazole perchlorate)，分子量881.72 ，是一種具有親脂性(lipophilic property)的長鏈二烷基碳花青染料螢光染料( dialkyl carbocyanine dye fluorescent dye)。它通常用於標記細胞膜和其他脂溶性生物結構。進入細胞膜後，DiO可以橫向擴散並逐漸染色整個(cell membrane)。 DiO螢光在進入細胞膜(cell membrane)之前非常弱，但與細胞結合後，螢光強度會大大增強。 DiO受激發後可發出綠色螢光，可用標準FITC濾光片檢測。 DiO的最大激發波長為484nm，最大發射波長為501nm。根據DiO的特性，它既可用於活細胞染色，也可用於固定細胞或組織的染色。此外，DiO探針一般不會影響細胞的活力，因此可以使用正向或反向標記的細胞或某些物質（外泌體）作為示蹤劑來檢測。
本產品DiO細胞膜(cell membrane)綠色螢光染色試劑盒含有DiO螢光探針及優化的染色緩衝液，可使細胞膜(cell membrane)染色更快，螢光更明亮穩定，效果更好。
| Component Number | Component | G1704 |
| G1704-1 | DiO cell membrane green fluorescent probe | 100μL |
| G1704-2 | Staining buffer | 100mL |
| Manuals | One copy | |
Assay Protocol
1. Preparation of DiO staining working solution:
1.1. DiO staining working solution (prepared when using) was prepared by mixing and diluting DiO cell membrane green fluorescent probe with staining buffer at a ratio of 1:1000; Note that the Dio probe dilution ratio can be adjusted from 1:500 to 1:2000 according to the specific situation to obtain the best staining effect.
2. Staining of suspension of live cell:
2.1. The suspended cells were collected and centrifuged at 500-1000 g at room temperature for 3-5 min to remove the cell supernatant;
2.2. Cells were resuspended with DiO staining working solution to make the cell density at 1-2×106 cells /mL;
2.3. Incubate at 37℃ for 5-20 min in the dark; It is recommended to adjust the staining time according to the specific cells to obtain the best staining effect.
2.4. At the end of incubation, centrifuge 500-1000 g at room temperature for 3-5 min to aspirate DiO staining working solution;
2.5. Resuspended cells with 37℃ preheated PBS or cell medium, centrifuged at 500-1000 g for 3-5 min, and removed supernatant;
2.6. Repeat Step 2.5 once;
2.7. The cells were detected by flow cytometry, or transferred to porous plates, cell culture dishes or slides, and observed under fluorescence microscopy. The maximum excitation wavelength of Dio is 484nm, and the maximum emission wavelength is 501nm.
3. Staining of adherent live cells (6-well plate as an example):
3.1. The cells were planted in 6-well plates at a certain density in advance;
3.2. The cell medium was removed by aspiration, and the cells were washed twice with PBS (G4202). 1 mL DiO staining working solution was added (well plates with other specifications were adjusted according to the situation to ensure dye coverage of cells);
3.3. Incubate at 37℃ for 5-20 min in the dark, and remove DiO staining working solution by suction. It is suggested to adjust the staining time according to the specific cells to obtain the best staining effect.
3.4. Wash the cells 1-2 times with 37℃ preheated PBS or cell medium;
3.5. Add PBS preheated at 37℃ or cell medium and observe under fluorescence microscope. The maximum excitation wavelength of DiO is 484nm, and the maximum emission wavelength is 501nm.
4. Staining of fixed adherent cells or sections:
4.1. Sample pretreatment:
For cells: Remove the cell medium and wash 1-2 times with PBS. 4% paraformaldehyde fixing solution (G1101) was added and fixed for 10 min at room temperature. Remove fixation solution by suction and wash with PBS 2-3 times.
For tissue sections: paraffin sections are dewaxed; Frozen sections were rewarmed and washed with water.
4.2. Cells or sections were treated by adding 0.1-0.5% Triton-100 (prepared with PBS) and permeated for 10 min at room temperature; The permeable liquid was removed and washed with PBS 2-3 times;
4.3. (Optional, immunofluorescent labeling) Incubation of antibodies or staining with other dyes according to immunofluorescent staining method. Note: In the process of antibody incubation, the blocking solution, antibody diluent, washing solution and so on should not contain scaling agent.
4.4. Add an appropriate volume of DiO staining working solution to cover cells or tissues, incubate at 37°C for 5-20 min in the dark, and remove DiO staining working solution. It is recommended to adjust the staining time according to the specific cell or tissue samples to obtain the best staining effect.
4.5. Cells or sections were washed with PBS for 2-3 times and then placed under a fluorescence microscope (cells should be covered with appropriate amount of PBS). The maximum excitation wavelength of DiO is 484nm, and the maximum emission wavelength is 501nm.
5. Fluorescent labeling of exosomes:
5.1. The extracted exosomes were precipitated and resuspended with an appropriate amount of DiO staining working solution;
5.2. Incubate the sample at 37℃ for 30 min in the dark;
5.3. Dilute the sample volume with 10 times the volume of PBS (optional);
5.4. Exosomes were extracted again according to the previous extraction protocol to remove excess dyes;
5.5. The collected exosomes were precipitated and resuspended in PBS to obtain DIO-labeled exosomes. It can be used for subsequent experiments, such as cellular uptake.
Fluorescence Microscopy to Observe The Procedure of Living Dead Cells：
1. All fluorescent dyes have quenching problems, please pay attention to avoid light to slow down fluorescence quenching.
2. Due to the lipophilic characteristics of the probe, please avoid using reagents containing glycerol or other organic matter during the experiment, otherwise the staining effect will be affected.
3. When fixing operation is required, the sample should be fixed in 4% paraformaldehyde, because other inappropriate fixing solutions will lead to high fluorescence background.
4. The optimum dilution ratio and incubation time of the probe should be adjusted according to the actual situation due to the different sensitivity between cells and experimental purposes.
5. For your health and safety, please wear laboratory coat and gloves when operating.
流式細胞儀偵測細胞週期程序：
1. DiO染色工作液的配製：
1.1. DiO染色工作液（使用時配製）將DiO細胞膜綠色螢光探針與染色緩衝液以1:1000的比例混合稀釋而成；注意，Dio探針稀釋比例可依具體情況在1:500至1:2000之間調整，以獲得最佳染色效果。
2.活細胞懸浮液染色：
2.1.收集懸浮細胞，室溫500-1000g離心3-5min，除去細胞上清；
2.2.以DiO染色工作液重懸細胞，使細胞密度為1-2×106個/mL；
2.3. 37℃避光孵育5-20分鐘；建議根據特定細胞調整染色時間，以獲得最佳染色效果。
2.4.孵育結束，室溫500-1000 g離心3-5 min，吸去DiO染色工作液；
2.5.以37℃預熱的PBS或細胞培養基重懸細胞，500-1000 g離心3-5 min，去除上清；
2.6。重複步驟2.5一次；
2.7.透過流式細胞儀檢測細胞，或轉移至多孔板、細胞培養皿或玻片上，並在螢光顯微鏡下觀察。 Dio的最大激發波長為484nm，最大發射波長為501nm。
3.貼壁活細胞染色（以6孔板為例）：
3.1.將細胞預先以一定密度種植於6孔板中；
3.2.透過抽吸除去細胞培養基，並以PBS (G4202)洗滌細胞兩次。加入1 mL DiO染色工作液（其他規格孔板依情況調整，以確保染料覆蓋細胞）；
3.3. 37℃避光孵育5-20 min，吸去DiO染色工作液。建議根據特定細胞調整染色時間，以獲得最佳染色效果。
3.4.以37℃預熱的PBS或細胞培養基洗滌細胞1-2次；
3.5.加入37℃預熱的PBS或細胞培養基，在螢光顯微鏡下觀察。 DiO的最大激發波長為484nm，最大發射波長為501nm。
4.固定貼壁細胞或切片的染色：
4.1.樣品預處理：
細胞：移除細胞培養基並以 PBS 洗滌 1-2 次。加入4%多聚甲醛固定液（G1101），室溫固定10分鐘。吸除固定液，用 PBS 清洗 2-3 次。
對於組織切片：石蠟切片脫蠟；將冰凍切片重新加熱並用水洗滌。
4.2.細胞或切片加入0.1-0.5% Triton-100（PBS配製）處理，室溫滲透10 min；除去滲透液，用PBS洗滌2-3次；
4.3. （可選，免疫螢光標記）根據免疫螢光染色方法孵育抗體或用其他染料染色。注意：抗體孵育過程中，封閉液、抗體稀釋液、洗滌液等不得含有結垢劑。
4.4.加入適量 DiO 染色工作液覆蓋細胞或組織，37℃避光孵育 5-20 分鐘，除去 DiO 染色工作液。建議根據特定細胞或組織樣本調整染色時間，以獲得最佳染色效果。
4.5.細胞或切片以PBS洗滌2-3次，然後置於螢光顯微鏡下（細胞應覆蓋適量的PBS）。 DiO的最大激發波長為484nm，最大發射波長為501nm。
5. 外泌體螢光標記：
5.1.萃取的外泌體以適量的DiO染色工作液沉澱重懸；
5.2. 37℃避光孵育樣品30分鐘；
5.3.以10倍體積的PBS（可選）稀釋樣品體積；
5.4.依照先前的萃取方案再次萃取外泌體，去除多餘的染料；
5.5.將收集的外泌體沉澱並重懸於PBS中以獲得DIO標記的外泌體。可用於後續實驗，例如細胞攝取。
螢光顯微鏡觀察活死細胞的過程：
1.所有螢光染料都有猝滅問題，請注意避光以減緩螢光猝滅。
2. 由於探針的親脂特性，實驗過程中請避免使用含有甘油或其他有機物的試劑，否則會影響染色效果。
3. 需要固定操作時，樣本應在4%多聚甲醛中固定，因為其他不合適的固定液會導致螢光背景過高。
4.由於細胞之間的靈敏度和實驗目的不同，探針的最佳稀釋比例和孵育時間應根據實際情況進行調整。
5.為了您的健康與安全，操作時請穿戴實驗服和手套。
|
Cat.No.
|
Product Name
|
Spec.
|
|---|
|
G1011-10ML
|
Hoechst 33258 Staining Solution (Ready-To-Use)
Hoechst 33258 染色液（即用型） |
10 mL
|
|
G1012-100ML
|
DAPI Staining Reagent (Ready to Use)
DAPI 染色試劑（即使用型） |
100 mL
|
|
G1012-10ML
|
DAPI Staining Reagent (Ready to Use)
DAPI 染色試劑（即使用型） |
10 mL
|
|
G1021-10ML
|
PI Stain
碘化丙啶(propidium iodide, PI) |
10 mL
|
|
G1127-1ML
|
Hoechst 33342 Staining Solution
Hoechst 33342 染色液 |
1 mL
|
|
G1515-100T
|
JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 粒線體膜電位檢測試劑盒 |
100 T
|
|
G1704
|
DiO Cell Membrane Green Fluorescence Staining Kit
DiO 細胞膜綠色螢光染色試劑盒 |
100-1000 T
|
|
G1705
|
Dil Cell Membrane Red Fluorescence Staining Kit
Dil 細胞膜紅色螢光染色試劑盒 |
100-1000 T
|
|
G1720-20UL
|
ER-Tracker Green
ER-追蹤器綠色(ER-Tracker Green) |
20 μL
|
|
G1721-20UL
|
ER-Tracker Red
ER-追蹤器紅色(ER-Tracker Red) |
20 μL
|
|
G1722-50UL
|
Lyso-Tracker Green
溶血追蹤器綠色(Lyso-Tracker Green) |
50 μL
|
|
G1723-50UG
|
MitoTracker Red CMXRos
MitoTracker 紅色 |
50 μg
|
|
G1905-100T
|
Lipid Droplets Fluorescence Assay Kit
脂滴螢光檢測試劑盒 |
100 T
|
Servicebio台灣品牌介紹-BRAND/AGENCY/品牌介紹- Servicebio
| Classfication | Cat No. | Name | Place of Origin | Spec. |
|
Amino Acids
|
GC304002 | L-Glutamine | 產品資料 | 100 g |
| GC304003 | L-Arginine hydrochloride | 產品資料 | 25 g | |
| GC304004-100g | L-Glutamine acid | 產品資料 | 100 g | |
| GC304005 | L-Isoleucine | 產品資料 | 25 g | |
| GC304006-25g | L-Leucine | 產品資料 | 25 g | |
| GC304007 | L-Lysine hydrochloride | 產品資料 | 25 g | |
| GC304009 | L-Methionine | 產品資料 | 25 g | |
| GC304010 | L-Phenylalanine | 產品資料 | 10 g | |
| GC304011-25g | L-Alanine | 產品資料 | 25 g | |
| GC304012 | L-Histidine | 產品資料 | 25 g | |
| GC304013 | L-Serine | 產品資料 | 25 g | |
| GC304014 | L-tryptophan | 產品資料 | 5 g | |
| GC304016 | L-Threonine | 產品資料 | 25 g | |
| GC304017 | L-Valine | 產品資料 | 10 g | |
| GC304019 | Glycine | 產品資料 | 500 g | |
| GC304021 | L-Histidine hydrochloride monohydrate | 產品資料 | 25 g | |
| GC304022 | Glycine cell culture grade 甘氨酸Glycine (細胞培養等級 |
產品資料 | 100 g | |
|
Protein Derivatives
|
GC305002-5g | Iodoacetamide | 產品資料 | 5 g |
| GC305003 | Trypsin 1:250 Source: Porcine Pancreas | 產品資料 | 10 g | |
| GC305004-5g | Pepsin 1:3000 | 產品資料 | 5 g | |
| GC305005 | Horseradish peroxidase | 產品資料 | 1 g | |
| GC305006 | Bovine Serum Albumin – import | 產品資料 | 100 g | |
| GC305009 | Human Albumin-FITC | 產品資料 | 0.5 mL | |
| GC305010 | Bovine Serum Albumin BSA | 產品資料 | 100 g | |
| GC305010-25G | Bovine Serum Albumin BSA | 產品資料 | 25 g | |
| GC305010-50G | Bovine Serum Albumin BSA | 產品資料 | 50 g | |
| GC305010-5G | Bovine Serum Albumin BSA | 產品資料 | 5 g | |
| GC305013-100mg | Collagenase I | 產品資料 | 100 mg | |
| GC305014-100mg | Collagenase II | 產品資料 | 100 mg | |
| GC305015-100mg | Collagenase IV 膠原酶IV (Collagenase IV) |
產品資料 | 100 mg | |
| GC305016-100g | Gelatine | 產品資料 | 100 g | |
|
Hormones
|
GC302002-25mg | Abscisic acid | 產品資料 | 25 mg |
| GC302003 | Angiotensin II | 產品資料 | 10 mg | |
|
Biological pigments
|
GC307002-25g | Crystal violet | 產品資料 | 25 g |
| GC307003-5g | Trypan Blue | 產品資料 | 5 g | |
| GC307005 | Alcian Blue8GX | 產品資料 | 1 g | |
| GC307006-5g | Bromphenol Blue | 產品資料 | 5 g | |
| GC307007 | Serva blue G-250 | 產品資料 | 10 g | |
| GC307008-5g | Congo Red | 產品資料 | 5 g | |
| GC307009-25g | Alizarin Red S | 產品資料 | 25 g | |
| GC307010-1g | Giemsa′s Stain | 產品資料 | 1 g | |
| GC307011 | Fuchsin basic | 產品資料 | 5 g | |
| GC307012-10g | Ponceau S | 產品資料 | 10 g | |
| GC307013 | Acid red 26 2R | 產品資料 | 5 g | |
| GC307014 | Direct Red 80 | 產品資料 | 5 g | |
| GC307015-5g | Sudan Black B | 產品資料 | 5 g | |
| GC307017 | Aniline blue | 產品資料 | 5 g | |
| GC307018 | Nuclear fast red | 產品資料 | 5 g | |
| GC307019 | Solvent Blue 38 | 產品資料 | 5 g | |
| GC307020 | Hematoxylin | 產品資料 | 5 g | |
| GC307021 | Solvent Red 43 | 產品資料 | 10 g | |
| GC307022 | Eosin Y, water soluble | 產品資料 | 10 g | |
| GC307023 | Safranin O | 產品資料 | 10 g | |
| GC307024 | Fast Green | 產品資料 | 10 g | |
| GC307026-5g | Pyronin G | 產品資料 | 5 g | |
| GC307027-1g | Janus Green B | 產品資料 | 1 g | |
| GC307030-10g | Brilliant Cresyl Blue | 產品資料 | 10 g | |
| GC307031 | Coomassie brilliant blue R-250 | 產品資料 | 5 g | |
| GC307034-1g | Thionine acetate | 產品資料 | 1 g | |
| GC307035-1g | Cresyl Fast Violet | 產品資料 | 1 g | |
| GC307036-1g | Orcein | 產品資料 | 1 g | |
| GC307037-1g | Carmine | 產品資料 | 1 g | |
| GC307039-10g | Mordant Blue 29 | 產品資料 | 10 g | |
| GC307042-5g | Oil Red O | 產品資料 | 5 g | |
| GC307045-25g | Rhodamine B | 產品資料 | 25 g | |
|
Vitamins
|
GC301015-25g | Vitamin C | 產品資料 | 25 g |
| GC303001 | Folic acid | 產品資料 | 5 g | |
| GC303004 | Ribofavin | 產品資料 | 25 g | |
| GC303005-5g | VitaminB6 | 產品資料 | 5 g | |
| GC303006-25g | VitaminB3 | 產品資料 | 25 g | |
| GC303008 | VitaminB12 | 產品資料 | 1 g | |
| GC303011-25mg | NADPH-Na4 | 產品資料 | 25 mg | |
| GC303013-250mg | β-nicotinamide adenine dinucleotide NAD | 產品資料 | 250 mg | |
| GC303014-250mg | NADH-Na2 | 產品資料 | 250 mg | |
|
Antibiotics
|
GC301002 | Streptozocin STZ | 產品資料 | 1 g |
| GC301002-100mg | Streptozocin STZ | 產品資料 | 100 mg | |
| GC301003 | Penicillin G Sodium salt | 產品資料 | 1 g | |
| GC301004 | Ampicillin | 產品資料 | 1 g | |
| GC301005 | Streptomycin sulfate | 產品資料 | 5 g | |
| GC301008 | Kanamycin | 產品資料 | 1 g | |
| GC301009 | Kanamycin sulfate | 產品資料 | 5 g | |
| GC301009-1g | Hygromycin B | 產品資料 | 1 g | |
| GC301011 | Geneticin G-418 | 產品資料 | 1 g | |
| GC301013 | Chloramphenicol | 產品資料 | 5 g | |
| GC301013-10mg | Pseudomonic acid | 產品資料 | 10 mg | |
| GC301014-5g | Norfloxacin | 產品資料 | 5 g | |
| GC301016 | Cisplatin | 產品資料 | 100 mg | |
| GC301017-100mg | Sirolimus | 產品資料 | 100 mg | |
|
Heterocyclic compounds
|
GC207001 | Imidazole | 產品資料 | 10 g |
| GC207002 | PMSF | 產品資料 | 5 g | |
| GC207003 | Freund’s Adjuvant Complete | 產品資料 | 10 mL | |
| GC207004 | Freund’s Adjuvant Incomplete | 產品資料 | 10 mL | |
|
Salt
|
GC201001 | Guanidine HCl | 產品資料 | 500 g |
| GC201002 | Guanidine thiocyanate | 產品資料 | 500 g | |
|
Sugar Alcohols
|
GC205001 | L(+)-Lactic acid | 產品資料 | 100 g |
| GC205002 | Pyruvic acid sodium salt | 產品資料 | 25 g | |
| GC205003 | D-Glucose, anhydrous | 產品資料 | 500 g | |
| GC205004-10g | D(+)-Trehalose dihydrate | 產品資料 | 10 g | |
| GC205005 | D(+)- Galactose | 產品資料 | 100 g | |
| GC205006-10g | D-(-)Arabinose | 產品資料 | 10 g | |
| GC205007-1g | DTE | 產品資料 | 1 g | |
| GC205008 | Glycerol | 產品資料 | 100 mL | |
| GC205009 | LPS | 產品資料 | 10 mg | |
| GC205010 | DTT | 產品資料 | 1 g | |
| GC205011 | IPTG | 產品資料 | 1 g | |
| GC205013 | Agarose | 產品資料 | 100 g | |
| GC205014 | Sucrose | 產品資料 | 500 g | |
| GC205015 | D-Sorbitol | 產品資料 | 500 g | |
|
Solvent
|
GC203001 | TEMED | 產品資料 | 100 mL |
| GC203002 | DMSO(Dimethyl sulfoxide) | 產品資料 | 100 mL | |
| GC203003 | Urea | 產品資料 | 500 g | |
| GC203005 | DMSO cellular level | 產品資料 | 10 mL | |
|
Complex
|
GC202001 | EDTA | 產品資料 | 500 g |
| GC202002 | EDTA -Na2 | 產品資料 | 500 g | |
|
Buffer salt
|
GC208001 | Tris (Hydroxymethyl) Aminomethane | 產品資料 | 500 g |
| GC208003 | RIS-HCL | 產品資料 | 500 g | |
| GC208004-100g | BIS-TRIS | 產品資料 | 100 g | |
| GC208006 | N-Tris[Hydroxymethyl]Methylglycine | 產品資料 | 100 g | |
| GC208007 | 4-propanesulfonyl morpholine | 產品資料 | 100 g | |
| GC208008-25g | HEPPS | 產品資料 | 25 g | |
| GC208009 | HEPES, Free acid | 產品資料 | 100 g | |
| GC208012-1g | TCEP·HCL | 產品資料 | 1 g | |
|
Surfactant
|
GC204001 | Tween-80 | 產品資料 | 100 mL |
| GC204002 | Tween-20 | 產品資料 | 100 mL | |
| GC204003 | Triton X-100 | 產品資料 | 100 mL | |
| GC204004 | Sodium dodecylsulfate | 產品資料 | 500 g | |
| GC204005 | Sodium dodecylsulfate | 產品資料 | 100 g | |
| GC204006 | Nonidet TM P40 Substitute NP-40 | 產品資料 | 100 g | |
| GC204007 | Deoxycholic acid， sodium salt | 產品資料 | 25 g | |
| GC204009-100ml | APES | 產品資料 | 100 mL | |
|
Amine organics
|
GC206002 | Acrylamide | 產品資料 | 500 g |
| GC206003 | N’-MBA | 產品資料 | 100 g | |
|
Polymer
|
GC209002-250g | PEG 8000 | 產品資料 | 250 g |
| GC209003-250g | PEG 6000 | 產品資料 | 250 g | |
| GC209004-100g | PEG 3350 | 產品資料 | 100 g | |
| GC209005-500g | Poly(ethylene glycol)2000 | 產品資料 | 500 g | |
| GC209006-250g | Poly(ethylene glycol) 20000 | 產品資料 | 250 g | |
|
Inorganic salt
|
GC106001 | Orthoboric acid | 產品資料 | 500 g |
| GC104004 | Sodium bicarbonate | 產品資料 | 500 g | |
| GC108003 | Citric acid monohydrate | 產品資料 | 100 g | |
| GC108004 | Sodium citrate dihydrate | 產品資料 | 100 g | |
| GC102001 | Calcium chloride | 產品資料 | 100 g | |
| GC102002 | Magnesium chloride hexahydrate | 產品資料 | 100 g | |
| GC102003 | Potassium chloride | 產品資料 | 500 g | |
| GC102004 | Sodium chloride | 產品資料 | 500 g | |
| GC101004 | Magnesium Sulfate | 產品資料 | 100 g | |
| GC101013 | Ammonium persulfate | 產品資料 | 25 g | |
| GC105001 | Sodium phosphate monobasic | 產品資料 | 500 g | |
| GC105002 | Sodium Phosphate, Dibasic | 產品資料 | 500 g | |
| GC105003 | Potassium Phosphate Monobasic | 產品資料 | 500 g | |
| GC107001 | Sodium hydroxide | 產品資料 | 100 g | |
| GC306001 | Lodixanol | 產品資料 | 5 g | |
| GC310001-100g | skimmed milk powder | 產品資料 | 100 g |