凋亡細胞分離試劑盒| Apoptotic Cell Isolation Kit貨號K258

產品描述: 凋亡細胞分離試劑盒(Apoptotic Cell Isolation Kit, K258)提供了一種簡單有效的方法，可使用膜聯蛋白V /磁珠(annexin V/magnetic beads)（MagBeads）從細胞培養物或組織製劑中分離凋亡細胞(apoptotic cells)或去除死細胞(dead cells)。膜聯蛋白V(Annexin V)是一種對Ca 2+依賴性的磷脂結合蛋白(Ca²ᶧ-dependent phospholipid binding protein)，對磷脂酰絲氨酸（phosphatidylserine , PS）具有高親和力，它從內質膜小葉重新分佈到凋亡或死細胞中。一旦進入細胞表面，PS就可用於結合膜聯蛋白V及其任何結合物。 Annexin V-生物素與凋亡細胞的結合，然後生物素與鏈黴親和素-MagBeads(streptavidin-MagBeads)的結合，使凋亡細胞(apoptotic)與活細胞分離。與MagBeads結合的凋亡細胞粘附在磁體上，而非凋亡細胞則保持懸浮狀態。然後可以將分離的凋亡細胞和健康細胞用於各種測定中，以研究凋亡機制和途徑。該試劑盒也已成功用於從健康細胞培養物中去除死細胞。

穀胱甘肽過氧化物酶活性測定 |  FRAP檢測 | 總膠原蛋白分析 | Dopamine多巴胺檢測 | 三酸甘油酯分析 | Cholesterol Efflux膽固醇排出螢光分析 | 脂質過氧化（MDA）測定 | 支鏈氨基酸 BCAA檢測

Cat # +Size	K258-30
Size	30 isolations
Detection Method	Magnetic bead bound apoptotic cells can be separated from normal cells
Species Reactivity	Mammalian
Applications	Kit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads (MagBeads). The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.
Features & Benefits	• Simple procedure; takes only ~1 hours • Fast and convenient
Kit Components	• Annexin V-Biotin • 1X Binding Buffer • Streptavidin-MagBeads • Apoptotic Cell Releasing Buffer
Storage Conditions	4°C
Shipping Conditions	Gel Pack

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