SARS-CoV-2 IgG ELISA檢測試劑 | 貨號E4901

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原廠連結：	https://www.biovision.com
相關下載：	SARS-CoV-2 IgG ELISA檢測試劑操作手冊

特點

SARS-CoV-2冠狀病毒介紹

冠狀病毒(Coronaviruses)是大型的脂質包裹(lipid-enveloped)的單鏈RNA病毒(single-stranded RNA viruses)，已知會感染多種宿主，包括人(humans)，蝙蝠(bats)，小鼠(mice)和其他哺乳動物(mammals)。在七種已知的人類冠狀病毒中，MERS-CoV，SARS-CoV-1和SARS-CoV-2是最致病的，會引起嚴重的，可能致命的呼吸道(respiratory)和心血管(cardiovascular)症狀。新型SARS-CoV-2病毒的出現是造成2019年冠狀病毒疾病（COVID-19）大流行的原因，已被證明是21世紀最大的公共衛生危機。 SARS-CoV-2具有〜30 kb的基因組，可編碼29種蛋白質，包括用於病毒體裝配的4種結構蛋白質，即包膜（Envelope , E），膜（Membrane , M），核仁（Nucleocapsid , N）和穗（Spike , S）蛋白質。跨膜刺蛋白(transmembrane Spike protein)是負責宿主細胞結合和病毒進入的糖蛋白(glycoprotein)。它是具有兩個獨特亞基的異三聚體複合物(heterotrimeric complex)。 S1亞基包含受體結合域（Receptor Binding Domain , RBD），它與血管緊張素轉化酶2（Angiotensin-Converting Enzyme 2 , ACE2）作為宿主細胞受體相互作用，而S2亞基(S2 subunit)介導病毒膜融合。 S蛋白是高度免疫原性的，通常在症狀發作後5-21天之間檢測到感染個體的血清轉化。

檢測產品介紹: SARS-CoV-2ELISA試劑盒 (貨號E4909) ，用於鑑定對SARS-CoV-2感染的免疫反應. BioVision的SARS-CoV-2 IgG ELISA試劑盒是一種酶聯免疫吸附測定，旨在鑑定對SARS-CoV-2感染的免疫反應。該測定法使用刺突蛋白S1亞基(spike protein S1 subunit)作為誘餌來捕獲稀釋的血漿或血清樣品中的抗SARS-CoV-2抗體。隨後與酶標記的抗人IgG綴合物(anti-human-IgG conjugate)和比色底物反應，產生顏色，該顏色與抗S1抗體的數量成比例。通過比較樣品的OD450 nm讀數與陰性對照血清的OD450 nm讀數來確定抗SARS-CoV-2 S1抗體（IgG）的存在。陽性反應表明先前的SARS-CoV-2感染始於≥14天前。試劑盒中包含含有重組人單克隆抗SARS-CoV-2 S1抗體的陽性對照血清，用於驗證。該測定法是一種指示最近或之前的SARS-CoV-2感染的快速，簡便和準確的方法，不需要收集或處理傳染性病毒。該試劑盒是研究COVID-19流行病學，患病率和發病率的寶貴工具。

Cat # +Size	E4901-100
Size	100 assays
Kit Summary	BioVision’s SARS-CoV-2 IgG ELISA Kit is an enzyme-linked immunosorbent assay intended for identifying the immune response to SARS-CoV-2 infection. The assay uses the spike protein S1 subunit as bait to capture anti-SARS-CoV-2 antibodies in diluted plasma or serum samples. Subsequent reaction with an enzyme-labeled anti-human-IgG conjugate and a colorimetric substrate generates color, which is proportional to the amount of anti-S1 antibodies. The presence of anti-SARS-CoV-2 S1 antibodies (IgG) is determined by comparing the OD450 nm reading of the sample to the OD450 nm reading of the negative control serum. A positive reaction suggests prior SARS-CoV-2 infection that began ≥14 days ago. Positive control serum containing a recombinant human monoclonal anti-SARS-CoV-2 S1 antibody is included in the kit for validation. The assay is a quick, easy and accurate method of indicating recent or prior SARS-CoV-2 infection that does not require collection or handling of infectious virus. This kit is a valuable tool to study the COVID-19 epidemiology, prevalence and morbidity.
Detection Method	Absorbance at 450 nm
Species Reactivity	Human
Applications	• Study of epidemiology, prevalence and morbidity of COVID-19 • Screening of SARS-CoV-2 infection rate in a given population • Determination of prior SARS-CoV-2 infection
Features & Benefits	• A quick, easy and accurate method of indicating recent or prior SARS-CoV-2 infection • A valuable tool to study the COVID-19 epidemiology, prevalence and morbidity • The kit does not require collection or handling of infectious virus
Kit Components	• Spike Protein-Coated ELISA Plate • Wash Buffer (10X) • Sample Diluent • Anti-IgG-HRP Conjugate (1000X) • Positive Control Serum • Negative Control Serum • TMB Substrate • Stop Solution • Plate Sealing Film
Storage Conditions	-20°C
Shipping Conditions	Gel Pack

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