PicoProbe™15-PGDH活性測定試劑盒（螢光法） | 15-PGDH Activity Assay Kit貨號K562

PicoProbe™ 15-PGDH Activity Assay Kit (Fluorometric)

產品介紹:. 15-羥基前列腺素脫氫酶（15-Hydroxy prostaglandin dehydrogenase , 15-PGDH; EC：1.1.1.141）是前列腺素分解代謝(prostaglandin catabolism.)的限速酶(rate-limiting enzyme)。它將前列腺素（prostaglandins , PG）氧化為15-酮代謝物(15-keto metabolites)。通常與PG相比，15-酮代謝物(15-keto metabolites) 的生物活性(biological activity)降低。 PGs是由花生四烯酸(arachidonic acid)通過環氧合酶（cyclooxygenase , COX）生成的，在炎症反應(inflammatory responses)中起重要作用。最近的研究發現15-PGDH可以作為PG的降解酶(degrading enzyme)，並可以抑制腫瘤(tumor suppressor)的發展。因此，對15-PGDH活性的研究可以闡明和闡明與癌症發展和治療有關的未知方面。在BioVision的15-PGDH檢測試劑盒中，15-PGDH氧化形成底物的中間體和NADH。 NADH的氧化減少了探針在Ex / Em = 535/587 nm處產生螢光。 15-PGDH的活性與螢光信號成正比。 BioVision的15-PGDH活性測定試劑盒提供了一種快速，簡單，靈敏和可靠的測試方法，適用於檢測低至1 µU的15-PGDH活性。

Cat # +Size	K562-100
Size	100 assays
Kit Summary	• Detection method- Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Application- The 15-PGDH fluorometric assay kit is a highly sensitive assay and can detect < 5 µU 12-PGDH activity (based on our unit definition) in a variety of samples.
Detection Method	Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Eukaryotes
Applications	• Measurement of 15-PGDH activity in various tissues/cells. • Analysis of prostaglandin catabolism in various cell types.
Features & Benefits	• Simple procedure • Fast and convenient • Sensitive assay for measuring 15-PGDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit Components	• 15-PGDH Assay Buffer • PicoProbeTM (in DMSO) • 15-PGDH Developer • ALDH Substrate (in DMSO) • NADH Standard • 15-PGDH Positive Control
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.

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