丙酮酸比色/螢光檢測試劑盒 | 貨號K609-100

Pyruvate Colorimetric/Fluorometric Assay Kit

產品描述：丙酮酸(Pyruvate)是代謝中的中心分子，糖通過該分子進入檸檬酸循環(citric acid cycle)。 丙酮酸(Pyruvate)可在糖異生過程中轉化(converted)為碳水化合物(carbohydrates)，或通過乙酰輔酶A轉化(acetyl CoA)為脂肪酸(fatty acids)。 高水平的丙酮酸(pyruvate)與肝臟疾病(liver disease)和遺傳性疾病(genetic disorders)有關。 丙酮酸也被用於刺激代謝導致體重減輕。 BioVision提供簡單，直接和自動化(automation-ready)的程序，用於測量各種生物樣品中的丙酮酸濃度(pyruvate concentration)，如血液(blood)，細胞(cells)，培養物(culture)和發酵培養基(fermentation media)等。在該測定中，丙酮酸被丙酮酸氧化酶(pyruvate oxidase)通過酶反應氧化產生顏色（λ = 570nm）和螢光（Ex / Em = 535 / 587nm）。 由於顏色螢光強度與丙酮酸(Pyruvate)含量成比例，因此可以精確地測量丙酮酸(Pyruvate)濃度。 該試劑盒檢測1μM至10 mM丙酮酸(Pyruvate)。

商品需用儀器測量  酵素免疫分析儀請按此 (標配405、450、492、630 nm, 可擴增至7個濾片)

 

 

 

 

 

 

 

穀胱甘肽過氧化物酶活性測定 |  FRAP檢測 | 總膠原蛋白分析 | Dopamine多巴胺檢測 | 三酸甘油酯分析 | Cholesterol Efflux膽固醇排出分析 | 脂質過氧化（MDA）測定 | 支鏈氨基酸 BCAA檢測

Cat # +Size	K609-100
Size	100 assays
Detection Method	Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity	Mammalian
Applications	The kit detects 1 µM to 10 mM pyruvate.
Features & Benefits	• Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples
Kit Components	• Pyruvate Assay Buffer • Pyruvate Probe (in DMSO) • Pyruvate Enzyme Mix • Pyruvate Standard (100 nmol/µl)
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.

引用文獻

Qi Wu, et al., ( 2018) Tumour-originated exosomal miR-155 triggers cancer-associated cachexia to promote tumour progression, Molecular Cancer, Oct. 2018, 30359265

Ana I. Duarte, et al., (2018) Dual Therapy with Liraglutide and Ghrelin Promotes Brain and Peripheral Energy Metabolism in the R6/2 Mouse Model of Huntington’s Disease, Scientific Reports, Jun. 2018, 29895889

Chem et al., Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome. J. Biol. Chem., Sep 2015; 290: 23240 – 23253.

Kim et al., Mice expressing reduced levels of hepatic glucose-6-phosphatase-α activity do not develop age-related insulin resistance or obesity.  Hum.  Mol. Genet., Sep 2015; 24: 5115 – 5125.

Chen et al., Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci. Rep., Jun 2015; 35: e00214.

乾浴加熱水浴槽 |  Finnpipette F3 微量分注器 |  試管振盪器 Vortex Mixer  |  Genereach迷你型微量離心機  |   ELISA儀器