3T3-L1分化試劑盒 |3T3-L1 Differentiation Kit貨號K579 (ABCAM ab287843)

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特點

心血管疾病相關研究-動脈粥樣硬化

心血管疾病（CVD）是全球發病和死亡的主要原因。 CVD的基礎包括對最終阻礙血液流動的心臟和血管的損害。這種廣泛的疾病包括冠狀動脈疾病（例如心肌梗塞或心臟病發作，心絞痛等），心律失常，中風，先天性心臟病和腎/血管疾病。 CVD的多重危險因素包括年齡，飲食，生活習慣變化，遺傳傾向和高血壓家族史，糖尿病和/或肥胖。 BioVision提供全面的產品組合，包括測定試劑盒，重組蛋白，抗體和生物化學品，以促進心血管生物學，藥物開發和生物標誌物發現研究。

3T3-L1分化試劑盒 |貨號K579 (ABCAM ab287843)

3T3-L1 Differentiation Kit

原理介紹: 3T3-L1細胞來自小鼠3T3細胞，提供廣泛使用的基礎模型，用於脂肪生理學和代謝疾病的研究。它們在分化前呈現成纖維細胞樣形態，但在分化開始後幾天變成圓形和積累脂滴。可以通過光學顯微鏡觀察累積的脂滴。體外分化的3T3-L1脂肪細胞產生與組織來源的脂肪細胞相似的特徵，並且通常用於研究脂肪形成，脂肪分解和代謝功能障礙。 BioVision的3T3-L1分化試劑盒提供足夠的補充劑，以製備100 ml分化培養基和600 ml維生素，這是12 100 mm培養皿的足夠材料。分化雞尾酒在分化培養基中提供1.5μg/ ml胰島素，1μM dexamethasone，500μMIBMX和1μM rosiglitazone的終濃度。

Cell Culture − Culture 3T3-L1 (ATCC® CL-173) in preadipocyte medium consisting of DMEM media with 10% bovine calf serum, 100 units/ml penicillin and 100 µg/ml streptomycin in a humidified incubator at 37°C with 5% CO2.

Δ Notes:

a) Important: Do not allow cultures to become confluent until initiation of differentiation to avoid overgrown culture. Change medium every 2-3 days and routinely split prior to initiating differentiation.

b) It is important to subculture preadipocytes in a medium with 10% bovine calf serum.

Assay Protocol Differentiation Induction:

1. To initiate differentiation, culture cells until ~80% confluent.

2. Replace medium with fresh preadipocyte medium and incubate an additional 48 hrs.

3. Add 1 µl of Differentiation Cocktail to 1 ml of DMEM/F12 (1:1) with 10% FBS. Make enough differentiation medium as needed. Sterilize with a 0.22 µM syringe filter. Replace preadipocyte medium with differentiation medium.

4. Incubate for 3 days in a humidified incubator at 37°C with 5% CO2. Δ Notes: a) It may be necessary to screen several lots of FBS, as some may be better at differentiation than others. b) Primary preadipocytes may differentiate better at 10% CO2.

Maintenance:

1. Prepare maintenance medium by adding 1 µl of Insulin to 1 ml of DMEM/F12 (1:1) with 10% FBS. Filter sterilize with 0.22 µM syringe filter.

2. Remove differentiation medium and replace with maintenance medium.

3. Replace medium every 2-3 days. − Lipid droplet accumulation will be visible by light microscopy 7-10 days after the addition of differentiation medium.

Cat # +Size	K579-100 (ABCAM ab287843)
Size	For 100 ml differentiation medium
Applications	Differentiation of 3T3-L1 preadipocytes to adipocytes Study of obesity, adipogenesis, lipolysis and lipid metabolism
Features & Benefits	• Simple, rapid & convenient method to differentiate 3T3-L1 preadipocytes to adipocytes
Kit Components	• Insulin (1.5 mg/ml) • Differentiation Cocktail 1000x (Lyophilized) • DMSO (anhydrous)

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