Cy5 NHS ester| CAS#146368-14-1 APExBIO貨號 A8108

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Cy5 NHS ester| CAS#146368-14-1 APExBIO貨號 A8108

Labeling of amino-groups in biomolecules.

產品介紹

Sulfo-Cy5 NHS ester 是一種磺化( sulfonated)、親水且水溶性極佳的染料。在水相系統中標記各種含氨基的分子時，無需使用有機助溶劑。該試劑特別適用於標記那些在有機助溶劑存在下易變性的蛋白質，以及溶解度較低的蛋白質。 Sulfo-Cy5 NHS ester 是最常使用的螢光團之一。在生命科學領域，磺酸基不僅賦予了 Cy 染料良好的水溶性，還能減少因染料分子間相互作用而導致的螢光猝滅。Sulfo-Cy5 NHS ester 與 LLP2A 進行了偶聯；在二甲基亞砜（DMSO）中，其激發/發射峰值分別為 646 nm 和 662 nm。 LLP2A 在細胞週邊呈現點狀染色，這與其標記 VLA-4 的特性相一致 [1]。

Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions. Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].

Storage	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
M.Wt	753.88
Cas No.	146368-14-1
Formula	C₃₇H₄₃N₃O₁₀S₂
Synonyms	CY5-SE; Sulfo-Cyanine5 Succinimidyl Ester
Solubility	insoluble in H2O; insoluble in EtOH; insoluble in DMSO
Chemical Name	2-((1E,3E)-5-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
Canonical SMILES	CC[N+]1=C(/C=C/C=C/C=C2N(CCCCCC(ON3C(CCC3=O)=O)=O)C(C=CC(S(O)(=O)=O)=C4)=C4C/2(C)C)C(C)(C)C5=C1C=CC(S([O-])(=O)=O)=C5
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

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