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長鏈游離脂肪酸呈色螢光分析套組 | Free Fatty Acid Quantification Kit貨號K612-100 (Abcam ab65341)

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原廠連結:
http://www.biovision.com
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游離脂肪酸呈色螢光分析套組操作手冊

特點

長鏈游離脂肪酸呈色螢光分析套組 Free Fatty Acid Quantification Colorimetric/Fluorometric Kit 

最低靈敏度2 µM

長鏈游離脂肪酸呈色螢光分析套組貨號K612-100  | 100個分析 (Abcam ab65341)

產品介紹:脂肪酸在正常代謝和許多疾病發展中起著非常重要的作用。 它們是諸如前列腺素,白三烯等一些生物活性類化合物的前體,並且涉及不同的功能,如自閉症,免疫系統和炎症反應。 BioVision的游離脂肪酸定量試劑盒為檢測各種生物樣品(如血清,血漿和其他體液,食物,生長介質等)中的長鏈游離脂肪酸提供了一種方便靈敏的基於酶的方法。 脂肪酸被轉化為它們的CoA衍生物,隨後伴隨著顏色或螢光的產生而被氧化。 然後可以通過比色法(在λ= 570nm處的分光光度法)或螢光法(在Ex / Em = 535 / 587nm處)方法容易地定量C-8(辛酸酯)和較長的脂肪酸,檢測限為2μM游離脂肪酸樣本。產品描述: Biovision提供一系列脂質分析套組;其中:長鏈游離脂肪酸呈色螢光分析套組,一組產品可選擇呈色Absorbance (570 nm)或螢光Fluorescence (Ex/Em 535/587 nm)兩種型態偵測Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm);可用於細胞培養液、body fluids、 food、growth media尿液、血漿、或液體型式的來源。操作時間大約40分鐘。測量最低靈敏度2 µM free fatty acid。

Free Fatty Acid Quantification Colorimetric/Fluorometric Kit | BioVision Inc.

功能研究 – 游離脂肪酸檢測試劑盒 – 定量 (ab65341)

通過使用 ab65341 測量釋放到 siCreg1 或 si-Scramble 3T3-L1 細胞培養基中的游離脂肪酸來評估脂解作用,這些細胞用 BMS-345541(5 µmol/L)、一種 NF-κB 通路抑製劑或載體處理( DMSO) 在脂肪分解評估前 48 小時。ns,無意義;並且 ***P<0.001。

ab65341 游離脂肪酸定量試劑盒

生物製品中測量的游離脂肪酸顯示濃度 (µM)。

游離脂肪酸呈色螢光分析套組 | K612-100內容物(Abcam ab65336)

  • Fatty Acid Assay Buffer
    • Fatty Acid Probe in DMSO
    • ACS Reagent
    • Enzyme Mix
    • Enhancer
    • Palmitic Acid Standard (1nmol/µl)
引用文獻
Marcel Nel, et al., (2018) Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines, Drug Design, Development and Therapy, Jun. 2018, 29983544
Kei Miyakawa, et al., (2018) Development of a cell-based assay to identify hepatitis B virus entry inhibitors targeting the sodium taurocholate cotransporting polypeptide., Oncotarget, May 2018, 29805766
Wenliang Zhang, et al., (2018) Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury, Scientific Reports, Feb. 2018, 29323242
Zhang W., et al., (2018) , Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury, Sci. Rep., 2018, 8:408
Cheng, C. F. et al., (2017) Monascus‐fermented red mold dioscorea protects mice against alcohol‐induced liver injury, while its metabolites ankaflavin and monascin regulates ethanol induced PPAR‐γ and SREBP‐1 expression in HepG2 cells, Journal of the Science of Food and Agriculture, Sep.2017,

游離脂肪酸呈色螢光分析套組

 Catalog #  Product Name+  Size
 K579-100  3T3-L1 Differentiation Kit  For 100 ml differentiation medium
 K658-100  Acetate Colorimetric Assay Kit  100 assays
 K650-100  Acetoacetate Colorimetric Assay Kit  100 assays
 K598-100  Acid Sphingomyelinase Activity Colorimetric Assay Kit  100 assays
 K610-100  Adipogenesis Colorimetric/Fluorometric Assay Kit  100 assays
 K4901-100  Adiponectin (human) Elisa Assay Kit  96 assays
 K4902-100  Adiponectin (mouse) Elisa Assay Kit  96 assays
 K4903-100  Adiponectin/gACRP30 (rat) Elisa Assay Kit  96 assays
 K731-100  Aldehyde Dehydrogenase Activity Colorimetric Assay Kit  100 assays
 K4696-100  Apolipoprotein E (human) ELISA Kit  100 assays
 K601-100  CETP Activity Fluorometric Assay Kit  100 assays
 K595-100  CETP Activity Fluorometric Assay Kit II  100 assays
 K602-100  CETP Inhibitor Drug Screening Kit (Fluorometric)  100 assays
 K594-100  CETP Inhibitor Screening Kit (Fluorometric) II  100 assays
 K582-100  Cholesterol Efflux Fluorometric Assay Kit (cell-based)  100 assays
 K7430-100  Cortisol (human/mouse/rat) ELISA Kit  100 assays
 K7434-100  Cortisol (Saliva) Human ELISA Kit  100 assays
 K612-100  Free Fatty Acid Quantification Colorimetric/Fluorometric Kit  100 assays
 K634-100  Free Glycerol Colorimetric Assay Kit II  100 assays
 K630-100  Free Glycerol Colorimetric/Fluorometric Assay Kit  100 assays
 K4921-100  FTO (human intracellular) ELISA Kit  100 assays
 K4922-100  FTO (mouse intracellular) ELISA Kit  100 assays
 K641-100  Glycerol-3-Phosphate (G3P) Colorimetric Assay Kit  100 assays
 K640-100  Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit  100 assays
 K613-100  HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit  100 assays
 K586-100  HDL Uptake Assay Kit (Fluorometric)  100 assays
 K584-100  Hepatic Steatosis Assay Kit  100 assays
 K588-100  HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric)  100 assays
 K632-100  β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit  100 assays
 K4761-100  Irisin Competitive ELISA Kit  100 assays
 K585-100  LDL Uptake Assay Kit (Fluorometric)  100 assays
 K4777-100  Leptin (human) ELISA Kit  100 assays
 K722-100  Lipase Activity Colorimetric Assay Kit  100 assays
 K723-100  Lipase Activity Colorimetric Assay Kit II  100 assays
 K724-100  Lipase Activity Fluorometric Assay Kit III  100 assays
 K580-24  Lipid (Oil Red O) staining Kit  24 ml
 K577-100  Lipolysis (3T3-L1) Colorimetric Assay Kit  100 assays
 K581-5  Lipolysis (Adipocyte) Colorimetric/Fluorometric Assay Kit  For 5 g Tissue
 K721-100  Lipoprotein Lipase Activity Fluorometric Assay Kit  100 assays
 K4909-100  Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit  100 assays
 K4908-100  Nampt (Visfatin/PBEF) (mouse/rat) Serum ELISA Kit  100 assays
 K766-100  PAF Acetylhydrolase (PAF-AH) Inhibitor Screening Kit (Colorimetric)  100 assays
 K765-100  PAF Acetylhydrolase Activity Assay Kit (Colorimetric)  100 assays
 K7265-100  PCSK9 (human) ELISA Kit  100 assays
 K7266-100  PCSK9 (mouse) ELISA Kit  100 assays
 K576-100  Phosphatidylcholine Colorimetric/Fluorometric Assay Kit  100 assays
 K725-100  Phospholipase D (PLD) Activity Colorimetric Assay Kit  100 assays
 K741-100  PicoProbe™ Aldehyde Dehydrogenase Activity Fluorometric Assay Kit  100 assays
 K673-100  PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit  100 assays
 K643-100  PicoProbe™ Free Glycerol Fluorometric Assay Kit  100 assays
 K651-100  PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit  100 assays
 K578-100  PicoProbe™ Lipolysis (3T3-L1) Fluorometric Assay Kit  100 assays
 K614-100  PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit  100 assays
 K593-100  PLTP Activity Fluorometric Assay Kit II  100 assays
 K605-100  PLTP Inhibitor Drug Screening Kit (Fluorometric)  100 assays
 K583-5  Preadipocyte Isolation Kit  For 5 g Tissue
 K4911-100  RBP4 (human) Competitive ELISA Kit  100 assays
 K4912-100  RBP4 (human) ELISA Kit  100 assays
 K4913-100  RBP4 (mouse/rat) ELISA Kit  100 assays
 K4767-100  Resistin (human/mouse/rat) EIA Kit  100 assays
 K600-100  Sphingomyelin Quantification Colorimetric Assay Kit  100 assays
 K599-100  Sphingomyelinase Activity Colorimetric Assay Kit  100 assays
 K574-100  Sphingomyelinase Activity Fluorometric Assay Kit  100 assays
 K623-100  Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II  100 assays
 K603-100  Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit  100 assays
 K622-100  Triglyceride Quantification Colorimetric/Fluorometric Kit  100 assays
 K4907-100  Visfatin (human) Elisa Assay Kit  100 assays

 

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