中性紅血球增殖檢測試劑盒| Neutral Red Cell Proliferation Assay Kit APExBIO貨號 K2262

A kit to detect cell proliferation by neutral red

產品介紹

中性紅血球增殖檢測試劑盒(Neutral Red Cell Proliferation Assay Kit )是一種可以快速檢測細胞增殖(cell proliferation )或毒性(toxicity)的試劑盒。細胞對中性紅(neutral red )的吸收取決於細胞維持 pH 梯度的能力，而 pH 梯度又取決於 ATP 的產生(ATP production)。在生理pH條件下( physiological pH conditions)，中性紅(neutral red )可藉由被動擴散(passive diffusion )進入細胞膜並在溶小體(lysosomes)中累積。當細胞數量增加時，中性紅(neutral red )的攝取量也會增加。相反，當細胞受損時，它們攝取中性紅(neutral red )的能力就會減弱。透過測量細胞攝取的中性紅(neutral red )的量，可以評估細胞的增殖或毒性。此外，中性紅(neutral red )色也可用作pH指示劑，其顏色會隨pH值而變化。中性紅(neutral red )在酸性環境中呈現紅色，當pH值從6.8上升至8.0時，顏色逐漸由紅色變成黃色。

1. Ready-to-use lysis buffer preparation:

Add 50 mL of absolute ethanol to the bottle of Neutral Red Lysis Buffer and shake to make the ready-to-use lysis buffer.

2. Cell culture:

For 96-well plates, seed cells at a density of 5-100×103 cells/well in 100 μL culture medium.Treat cells with the interested drug for a desired period. Prepare parallel wells as the background control (onlycontaining medium) and negative control (containing medium, cells and the same volume of solvent for theinterested drug)

3. Neutral red intake:

Prepare the neutral red staining solution referring to the table below. Remove the medium, add the freshly prepared neutral red staining solution, and incubate in the incubator for 2 h. The
incubation time can be adjusted according to the specific experiment and can be extended to 3-4 h.

4. Neutral red lysis:

Remove medium and wash 1-2 times with PBS. Add 200 μL of ready-to-use lysis buffer and lyse on a shaker at room temperature for 10 minutes.

5. Detection:

Measure the absorbance (A) at 540 nm with a microplate reader. A wavelength of 690 nm can be selected as the reference wavelength.

6. Analysis: Calculate cell viability with the following equation
Cell viability (%) =˷˄A Treatment sample – A Background control˅/˄A Negative control – A Background control˅˹x 100

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產品貨號	產品品項	反應數	產品介紹連結
K2249	MTT細胞增殖檢測試劑盒	500T/2500T	操作手冊
K2262	中性紅血球增殖檢測試劑盒	500T	操作手冊
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