孔雀石綠磷酸鹽測定試劑盒| Malachite Green Phosphate Assay Kit APExBIO貨號K2245

Malachite Green Phosphate Assay Kit

A kit for the detection of phosphate concentration and the ATPase activity

孔雀石綠磷酸鹽測定試劑盒(Malachite Green Phosphate Assay Kit, K2245) 是用來檢測磷酸鹽濃度的試劑盒。其原理是在酸性條件下，孔雀石綠(malachite green)、鉬酸鹽(molybdate)和游離無機磷酸鹽(free inorganic )或三磷酸鹽(green phosphomolybdate complex)可形成綠色磷鉬酸鹽絡合物(green phosphomolybdate complex)，這種綠色磷鉬酸鹽絡合物在650 nm處的吸光度與溶液中游離無機磷酸鹽的濃度成正比。此試劑盒可快速、方便、靈敏地測定溶液中游離無機磷酸鹽(free inorganic phosphate)，因此常用於溶液中磷酸鹽濃度和ATP酶活性(ATPase activity)的測定。但該試劑盒僅適用於溶液中游離磷酸鹽的測定。對於結合的磷酸鹽，例如與脂質或蛋白質結合，在檢測之前必須使用某些方法來釋放。試劑盒檢測時在0-10 nmol範圍內有良好的線性關係。

The Malachite Green Phosphate Assay Kit is a kit for the detection of phosphate concentration. The principle is that under acidic conditions, malachite green, molybdate and free inorganic or thophosphate can form a green phosphomolybdate complex, and the absorbance of this green phosphomolybdate complex at 650 nm is proportional to the concentration of free inorganic phosphate in solution.This kit can quickly, conveniently and sensitively determine free inorganic phosphate in solution, so it is often used for the determination of phosphate concentration in solution and the ATPase activity. However, this kit is only suitable for the determination of free phosphate in solution. For the bound phosphate, such as binding to lipids or proteins, certain methods must be used for release before detection. The kit has a good linear relationship in the range of 0-10 nmol during detection.

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產品貨號	產品品項	反應數	產品介紹連結
K2249	MTT細胞增殖檢測試劑盒	500T/2500T	操作手冊
K2262	中性紅血球增殖檢測試劑盒	500T	操作手冊
K2265	氫過氧化脂質 (LPO) 檢測試劑盒	100T	操作手冊
K2245	孔雀石綠磷酸鹽測定試劑盒	2500T	操作手冊