2-NBDG葡萄糖攝取檢測試劑盒| 2-NBDG Glucose Uptake Assay Kit APExBIO貨號 K2212
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2-NBDG葡萄糖攝取檢測試劑盒| 2-NBDG Glucose Uptake Assay Kit APExBIO貨號 K2212
2-NBDG Glucose Uptake Assay Kit
A kit for the detection of glucose uptake by the fluorescent probe 2-NBDG
葡萄糖代謝(Glucose metabolism)是將攝取的葡萄糖(glucose)轉化為能量的過程，也是生物體能量供應(energy supply)的主要來源。研究表明，葡萄糖代謝(glucose metabolism)與癌症、糖尿病、肥胖等多種疾病密切相關，因此監測葡萄糖攝取量非常重要。2-NBDG葡萄糖攝取檢測試劑盒是透過螢光探針2-NBDG檢測葡萄糖攝取的試劑盒。 2-NBDG 是一種螢光標記的葡萄糖類似物，它像葡萄糖一樣透過葡萄糖轉運蛋白 (GLUT) 運送到細胞內。 2-NBDG 一旦被細胞吸收，就會在 C-6 位磷酸化，產生 2-NBDG-6-磷酸，磷酸鹽可以很好地保留在細胞內。與其他葡萄糖追蹤器（2-DG 或 FDG）相比，2-NBDG 可以在單細胞層級原位檢測葡萄糖的吸收。該試劑盒提供了一種靈敏、快速、非放射性的檢測葡萄糖吸收的方法。同時，試劑盒提供了能夠顯著抑制葡萄糖攝取的GLUT1抑制劑phloretin作為陽性對照。對於96孔板，每孔使用100 μL工作液，該試劑盒至少可使用500次。
Glucose metabolism is a process that converts ingested glucose into energy, which is the main source of energy supply in living organisms. Studies have shown that glucose metabolism is closely related to many diseases such as cancer, diabetes and obesity, so it is important to monitor glucose uptake. The 2-NBDG Glucose Uptake Assay Kit is a kit for the detection of glucose uptake by the fluorescent probe 2-NBDG. 2-NBDG is a fluorescently labeled glucose analogue that is transported into cells by glucose transporter (GLUT) like glucose. Once 2-NBDG is uptaken in cells, it is phosphorylated at the C-6 position to produce 2-NBDG-6-phosphate, which is well retained in cells. Compared to other glucose trackers (2-DG or FDG), 2-NBDG allows in situ detection of glucose uptake at the single-cell level. This kit provides a sensitive, rapid and non-radioactive assay to detect glucose uptake. At the same time, the kit provides phloretin, a GLUT1 inhibitor that can significantly inhibit glucose uptake, as a positive control. For 96-well plates, using 100 μL of working solution per well, the kit can be used at least 500 times.
- 1. Lin Wu, Fei Huang, et al. “Interplay of FXN expression and lipolysis in white adipocytes plays a critical role in insulin sensitivity in Friedreich’s ataxia mouse model.” Sci Rep. 2024 Aug 27;14(1):19876 PMID: 39191875
- 2. Gang Zhao, et al. “The m6A modification-mediated positive feedback between glycolytic lncRNA SLC2A1-DT and c-Myc promotes tumorigenesis of hepatocellular carcinoma.” Int J Biol Sci. 2024 Feb 25;20(5):1744-1762. PMID: 38481809
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