NAD+/NADH 比色測定試劑盒 | Abcam 貨號(ab282929)
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Sirtuin 活性檢測試劑盒 | SIRT1 活性檢測試劑盒 | NMN | NAD+/NADH 比色測定試劑盒 | 細胞衰老檢測試劑盒 | Metformin | 雷帕黴菌（Rapamycin）
NAD+/NADH 比色測定試劑盒 | Abcam 貨號(ab282929)
NAD+/NADH Colorimetric Assay Kit (ab282929)
NAD+/NADH 比色測定試劑盒 (ab282929) 為靈敏檢測 NADH、NAD 及其比率提供了便利的工具。無需從樣品中純化 NADH 或 NAD。 NADH 選擇性顯色劑在與 NADH 反應時顯色，可在 OD 450 nm 處檢測。透過與提供的 NADH 標準品進行比較，可以輕鬆量化總 NAD。此方法定量、快速、簡單、靈敏，專為高通量格式而設計。此試劑盒可在 384 孔檢測板中檢測低至 0.04 pmol 的 NADH。此反應特異性檢測 NADH 和 NAD，但不檢測 NADP 或 NADPH。菸鹼醯胺腺嘌呤二核苷酸（Nicotinamide Adenine Dinucleotide, NAD）是多種催化蛋白活性不可或缺的輔酶。 NAD+ 和 NADH 及其還原形式參與許多生物氧化還原反應。 NAD+ 主要與分解代謝反應和能量產生所需的氧化還原酶有關。
將樣本離心並收集上清液並透過 10 kDa 旋轉柱脫蛋白。濾液中加入 10 pmol NADH 標準品，並依照試劑盒的方案進行測定。所有標準品和樣品的吸光度讀數均在室溫下循環反應 60 分鐘後取得。
新鮮處理的柱漿和內核組織中NADH的定量。試劑盒的方案製備組織勻漿，並以10 kDa旋轉柱（12000 xg，10分鐘，4°C）進行脫蛋白。試劑盒的方案對5 µl濾液進行測定。(c) 人體血清和體重中NADH的定量。
NADH、NAD和加熱NADH(Ht-NADH)和NAD(Ht-NAD)的曲線；兩者均在60°C下加熱30分鐘。
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Product name
NAD+/NADH Colorimetric Assay Kit
See all NAD/NADH kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals -
Product overview
This NAD+/NADH Colorimetric Assay Kit (ab282929) provides a convenient tool for a sensitive detection of NADH, NAD and their ratio. There is no requirement to purify NADH or NAD from samples. The NADH selective developer develops color while reacting with NADH and can be detected at OD 450 nm. The total NAD can be easily quantified by comparing with the provided NADH standard. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit can detect as low as 0.04 pmol of NADH in a 384 well assay plate. The reaction specifically detects NADH and NAD, but not NADP nor NADPH.
Sample types: Animal tissues: liver, kidney etc, Cell culture: adherent or suspension cells and Biological Fluids: Serum and Urine
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K958 EZScreen™ NAD+/NADH Colorimetric Assay Kit (384-well). K958-400 is the same size as the 400 test size of ab282929.
Nicotinamide Adenine Dinucleotide (NAD) is a co-enzyme indispensible for the activity of multiple catalytic proteins. NAD+ and NADH, its reduced form, participate in many biological redox reactions. NAD+ is mostly associated with redox enzymes required for catabolic reaction and energy production.
Storage instructions
| Components | Identifier | 400 tests |
|---|---|---|
| NAD Cycling Buffer | NM | 1 x 15ml |
| NAD Cycling Enzyme Mix | Green | 1 vial |
| NADH Developer | Purple | 1 vial |
| NADH Standard | Yellow | 1 vial |
| NADH/NAD Extraction Buffer | NM | 1 x 50ml |
| Stop Solution | Red | 1 x 1.2ml |
NAD（煙醯胺腺嘌呤二核苷酸）(Nicotinamide adenine dinucleotide, NAD) 是代謝氧化還原反應(metabolic redox reactions)中的輔酶、多種細胞訊號分子(cell signaling molecules)的前體以及蛋白質翻譯後修飾(protein posttranslational modifications)的底物。NAD (Nicotinamide adenine dinucleotide, NAD)是一種二核苷酸，由透過磷酸基連接的兩個核苷酸組成：一個核苷酸含有腺苷環，另一個含有菸鹼醯胺(nicotinamide)。在新陳代謝中，NAD (Nicotinamide adenine dinucleotide, NAD)參與氧化還原反應，將電子從一個反應傳遞到另一個反應。因此，輔酶在細胞中以兩種形式存在：NAD (Nicotinamide adenine dinucleotide, NAD)是一種氧化劑 – 它接受來自其他分子的電子並被還原，形成 NADH，然後可用作還原劑來提供電子。這些電子轉移反應是NAD (Nicotinamide adenine dinucleotide, NAD)的主要功能。然而，它也用於其他細胞過程，最值得注意的是在翻譯後修飾中添加或去除蛋白質化學基團的酶底物。
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