免疫沉澱套組 | Universal IP/Co-IP Toolkit (Magnetic Beads) 貨號 KTD104-EN
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免疫沉澱套組 | Universal IP/Co-IP Toolkit (Magnetic Beads) 貨號 KTD104-EN
Universal IP/Co-IP Toolkit (Magnetic Beads)
免疫沉澱（Immunoprecipitation，IP）是一種小型親和純化方法(affinity purification method)，使用固定在磁珠(magnetic beads)或瓊脂糖(agarose)等固相支持物(solid-phase supports)上的特異性抗體。免疫沉澱 (Immunoprecipitation, IP)作為許多蛋白質組學相關研究的重要組成部分，可用於檢測蛋白質(proteins)的存在、相對豐度(abundance)、蛋白質的上下表達、蛋白質的穩定性和相互作用等。Abbkine開發了兩款Universal IP/Co-IP Toolkit，磁珠(magnetic beads)或瓊脂糖(agarose)。 Universal IP/Co-IP Toolkit（Agarose）貨號 KTD105-EN；該工具包經過設計和研究，包含：優化的天然和變性裂解物(optimized natural and denatured lysate)、蛋白A/G瓊脂糖(protein A/G Agarose)、IP陰性對照(IP negative control)、IPkineTM二抗(second antibody)，可滿足大多數用戶的IP/Co-IP需求。通用 IP/Co-IP 工具包磁珠(magnetic beads)KTD104-EN；經過設計和研究，工具包包含：優化的天然(optimized natural)和變性裂解液( denatured lysate)、Protein A/G磁珠(protein A/G magnetic beads)、IP陰性對照(IP negative control)、IPkineTM二抗，可以滿足大多數用戶的IP/Co-IP需求。
Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.
高效(Efficient)：高效的抗體結合能力和低蛋白非特異性吸附率，節省抗體消耗。
- 方便通用(Convenient and universal-optimized natural and denatured lysate)：含優化的天然和變性裂解液，可同時滿足樣品IP/Co-IP 或WB 的要求。
- Protein A/G瓊脂糖具有更寬的結合範圍、更高效的抗體結合能力和更低的蛋白質非特異性吸附率，使操作更加省時、高效，節省抗體消耗。
- 可靠穩定(Reliable and stable)：含有即用型IP陰性對照(IP negative control)，可消除IgG自身與靶蛋白或其他特異性生物分子的非特異性結合，保證IP抗體的特異性，
- 並含有獨特的IPkine™二抗完美消除重鏈(heavy chain interference)干擾。
Specification
| Product name | Universal IP/Co-IP Toolkit (Magnetic Beads) |
| Applications notes | Based on the pain points of the traditional IP/Co-IP experiment, Abbkine developed a Universal IP/Co-IP Toolkit (Magnetic Beads); After designed and researched, the toolkit contains: optimized natural and denatured lysate, protein A/G magnetic beads, IP negative control, IPkineTM second antibody, it can meet the IP/Co-IP needs of most users |
Product Properties
| Kit components | • Non-Denaturing Lysis Buffer-25 mL • Denaturing Lysis Buffer-25 mL • 20×Wash Buffer-20 mL • Protein A/G Magnetic Beads-0.45 mL • Elution Buffer-2 mL • Neutralization Buffer-0.2 mL • 100×Proteinase Inhibitor Cocktail-0.2 mL • Mouse IgG (1 mg/mL)-30 μL • Rabbit IgG (1 mg/mL)-30 μL • IPKine™ HRP, Goat Anti-Mouse IgG LCS-30 μL • IPKine™ HRP, Mouse Anti-Rabbit IgG LCS-30 μL |
| Features & Benefits | • Efficient: Efficient antibody binding capacity and low protein non-specific adsorption rate, saving antibody consumption. • Convenient and universal: Contain all the necessary buffers needed for IP/Co-IP and WB experiments, it can meet the requirements of sample IP/Co-IP or WB at the same time. • Reliable and stable: It contains ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules to ensure the specificity of IP antibodies, and contains unique IPkine™ secondary antibodies to perfectly eliminate heavy chain interference. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Immunoprecipitation (Immunoprecipitation, IP) is a small affinity purification method using specific antibodies fixed on solid-phase supports such as magnetic beads or agarose. As an important part of many proteomics-related studies, IP can be used to detect the existence of proteins, relative abundance, up-and-down expression of proteins, stability and interaction of proteins and so on. |
Image & description
Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.
| Cat.No | Name | Size |
| KTD104-EN | Universal IP/Co-IP Toolkit (Magnetic Beads) | 20T |
| KTD105-EN | Universal IP/Co-IP Toolkit (Agarose) | 20T |
| KTI2014 | IPKine™ Anti-DDDDK Magnetic IP Kit | 20T/100T |
| KTI2024 | IPKine™ Anti-GFP Magnetic IP Kit | 20T/100T |
| KTI2034 | IPKine™ Anti-GST Magnetic IP Kit | 20T/100T |
| KTI2044 | IPKine™ Anti-HA Magnetic IP Kit | 20T/100T |
| KTI2064 | IPKine™ Anti-Myc Magnetic IP Kit | 20T/100T |
| ABT2010 | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL/200 μL/200 μL×5 |
| ABT2013 | Agarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL/5 mL |
| ABT2014 | Magnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL/5 mL |
| ABT2011 | Anti-DDDDK Tag Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABT2015 | HRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL/200 μL/200 μL×5 |
| ABT2020 | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL/200 μL/200 μL×5 |
| ABT2025 | HRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL/200 μL/200 μL×5 |
| ABT2021 | Anti-GFP Tag Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABT2024 | Magnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL/5 mL |
| ABT2023 | Agarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL/5 mL |
| ABT2030 | Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL/200 μL/200 μL×5 |
| ABT2035 | HRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL/200 μL/200 μL×5 |
| ABT2040 | Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL/200 μL/200 μL×5 |
| ABT2043 | Agarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL/5 mL |
| ABT2044 | Magnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL/5 mL |
| ABT2041 | Anti-HA Tag Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABT2045 | HRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL/200 μL/200 μL×5 |
| ABT2050 | Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL/200 μL/200 μL×5 |
| ABT2053 | Agarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL/5 mL |
| ABT2054 | Magnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL/5 mL |
| ABT2051 | Anti-His Tag Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABT2055 | HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL/200 μL/200 μL×5 |
| ABT2060 | Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL/200 μL/200 μL×5 |
| ABT2063 | Agarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL/5 mL |
| ABT2064 | Magnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL/5 mL |
| ABT2061 | Anti-Myc Tag Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABT2065 | HRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL/200 μL/200 μL×5 |
| ABT2170 | Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 50 μL/200 μL/200 μL×5 |
| ABT2173 | Agarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL/5 mL |
| ABT2174 | Magnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL/5 mL |
| ABT2070 | Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) | 50 μL/200 μL/200 μL×5 |
| ABT2080 | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | 50 μL/200 μL/200 μL×5 |
| ABT2090 | Anti-E2 Tag Mouse Monoclonal Antibody (12T4) | 50 μL/200 μL/200 μL×5 |
| ABT2100 | Anti-HSV Tag Mouse Monoclonal Antibody (16T2) | 50 μL/200 μL/200 μL×5 |
| ABT2110 | Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) | 50 μL/200 μL/200 μL×5 |
| ABT2120 | Anti-RFP Tag Mouse Monoclonal Antibody (9D1) | 50 μL/200 μL/200 μL×5 |
| ABT2130 | Anti-S Tag Mouse Monoclonal Antibody (9T10) | 50 μL/200 μL/200 μL×5 |
| ABT2150 | Anti-T7 Tag Mouse Monoclonal Antibody (6D4) | 50 μL/200 μL/200 μL×5 |
| ABT2160 | Anti-Trx Tag Mouse Monoclonal Antibody (14D4) | 50 μL/200 μL/200 μL×5 |
| ABT2180 | Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) | 50 μL/200 μL/200 μL×5 |
| ABT2190 | Anti-CBP Tag Monoclonal Antibody (12H5) | 50 μL/200 μL/200 μL×5 |
| ABT2200 | Anti-TAP Tag Monoclonal Antibody (4H2) | 50 μL/200 μL/200 μL×5 |
| ABT2210 | Anti-Avi-Tag Monoclonal Antibody (5G11) | 50 μL/200 μL/200 μL×5 |
| ABT2220 | Anti-SRT-Tag Monoclonal Antibody (11G3) | 50 μL/200 μL/200 μL×5 |
| ABT2240 | Anti-mStrawberry Monoclonal Antibody (4C9) | 50 μL/200 μL/200 μL×5 |
| ABT2250 | Anti-EYFP-Tag Monoclonal Antibody (10T3) | 50 μL/200 μL/200 μL×5 |
| ABT2260 | Anti-mOrange Monoclonal Antibody (9A10) | 50 μL/200 μL/200 μL×5 |
| ABT2270 | Anti-AmCyan Monoclonal Antibody (8T2) | 50 μL/200 μL/200 μL×5 |
| ABT2280 | Anti-ECFP-Tag Monoclonal Antibody (6B11) | 50 μL/200 μL/200 μL×5 |
| ABT2290 | Anti-EBFP Monoclonal Antibody (8B5) | 50 μL/200 μL/200 μL×5 |
| ABT2300 | Anti-Nano-Tag9 Monoclonal Antibody (11T3) | 50 μL/200 μL/200 μL×5 |
| A25012 | IPKine™ HRP, Goat Anti-Mouse IgG LCS | 100 μL/500 μL |
| A25022 | IPKine™ HRP, Mouse Anti-Rabbit IgG LCS | 100 μL/500 μL |
| A25112 | IPKine™ HRP, Goat Anti-Mouse IgG HCS | 100 μL/500 μL |
| A25122 | IPKine™ HRP, Mouse Anti-Rabbit IgG HCS | 100 μL/500 μL |
| A25222 | IPKine™ HRP, Goat Anti-Rabbit IgG HCS | 100 μL/500 μL |
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