IPKine™ HRP、山羊抗小鼠 IgG HCS| IPKine™ HRP, Goat Anti-Mouse IgG HCS Abbkine貨號A25112
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IPKine™ HRP、山羊抗小鼠 IgG HCS| IPKine™ HRP, Goat Anti-Mouse IgG HCS Abbkine貨號A25112 0.5 mg/ml
IPKine™ HRP, Goat Anti-Mouse IgG HCS
免疫沉澱(Immunoprecipitation)後蛋白質印跡(Western blots)上的 50kD 或 25kD 蛋白質檢測通常會受到重鏈(heavy chain )或輕鏈( light chain)印跡污染。 Abbkine 的 IPKine™ 產品通過減少背景噪音( less background noise)和提高準確性(enhanced accuracy)來解決這些問題。它們的輕鏈/Fc 片段特異性優化( light chain/Fc fragment specific optimization)將消除重鏈(heavy chain )或輕鏈( light chain)干擾，而吸收的血清蛋白(serum proteins absorbed)最大限度地減少與印跡上可能存在的其他物種免疫球蛋白(immunoglobulins)的交叉反應。IPKine™ HRP, Goat Anti-Mouse IgG HCS該抗體與小鼠 IgG 重鏈的 Fc 部分發生反應，但不與小鼠 IgG 的 Fab 部分發生反應。它與 human、bovine和馬血清蛋白(horse serum protein)的交叉反應已優化，但它可能與其他物種的免疫球蛋白發生交叉反應。它對非免疫球蛋白血清蛋白(non-immunoglobulin serum proteins)沒有反應性。
產品介紹: 山羊抗Mouse二級抗體，免疫原Mouse IgG Fc Fragment。可應用於ELISA, IHC-P, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting WB (1:1000-1:10000), IHC-P (1:50-1:500), ELISA (1:500-1:10000).。使用固相小鼠 IgG (H&L) Fc Fragment進行親和純化，最終基於 SDS-PAGE 的純度 > 95%。保存於Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
Specification
| Product name | IPKine™ HRP, Goat Anti-Mouse IgG HCS |
| Immunogen | Mouse IgG Fc Fragment |
| Host | Goat |
| Reactivity | Mouse |
| Applications | ELISA, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:10000), IHC-P (1:50-1:500), ELISA (1:500-1:10000). |
| Clonality | Polyclonal |
| Preparation method | Affinity purified using solid phase Mouse IgG(H+L) Fc Fragment with finally > 95% purity based on SDS-PAGE |
| Conjugate | HRP |
Product Properties
| Formulation | Liquid solution |
| Features & Benefits | 50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine’s IPKine™ products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots). |
| Storage buffer | Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. |
| Others | The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It’s cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins |
二抗(secondary antibody)是指用於靶向結合一抗的抗體。Abbkine 二抗可與酵素(HRP)、生物素(Biotin)或螢光團偶聯(DyLight)，用於各種基於抗體的應用，包括蛋白質印跡、免疫組織化學、免疫螢光、流式細胞術和 ELISA。，二抗( secondary antibody)和一抗聯合使用，檢測目的蛋白(target protein)，增強檢測信號(detection signal)。我們為您的每種應用提供來自山羊、兔和驢的高質量二抗。也可提供血清吸附二抗，推薦用於富含免疫球蛋白的樣品。Abbkine提供的二抗產品包括常用的酶標二抗（如HRP標記二抗）、DyLight螢光二抗、IFKine™二抗（取自驢，經其他物种血清吸附，具有較低的非-特異性結合和背景。特別適用於免疫學多標記實驗），IPKine™二抗（有效避免重鍊或輕鏈干擾，特別適用於IP-WB檢測）。
To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
要將 DyLight Fluors 與螢光成像儀一起使用，請為 DyLight 405 使用藍色激光二極管的光譜線，為 DyLight 488 使用青色 (488 nm) 激光，為 DyLight 550 和 594 使用綠色 (526 nm) 激光，以及紅色 ( 633 nm) 激光用於 DyLight 633 和 650。DyLight 680、755 和 800 螢光與基於激光和濾光片的紅外成像儀器兼容，這些儀器分別在 700 nm、750 nm 和 800 nm 光譜區域發射。
預染protein marker | 快速型蛋白質染色劑 | 活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色
Abbkine提供一系列高度特異性單克隆和多克隆抗體，tag antibody抗體系列商品如 His tag、GST tag、S tag、HA tag、DDDDK tag、Myc tag、MBP tag和 mCherry tag等單/多株Tag抗體。另具生物素(Biotin)、HRP、AbFluor™ 染料、瓊脂糖(agarose)和磁珠偶聯物(magnetic beads)標誌。
| Cat.No | Name | Size1 |
| KTD104-EN | Universal IP/Co-IP Toolkit (Magnetic Beads) | 20T |
| KTD105-EN | Universal IP/Co-IP Toolkit (Agarose) | 20T |
| A25012 | IPKine™ HRP, Goat Anti-Mouse IgG LCS | 100 μL |
| A25022 | IPKine™ HRP, Mouse Anti-Rabbit IgG LCS | 100 μL |
| A25112 | IPKine™ HRP, Goat Anti-Mouse IgG HCS | 100 μL |
| A25122 | IPKine™ HRP, Mouse Anti-Rabbit IgG HCS | 100 μL |
| A25222 | IPKine™ HRP, Goat Anti-Rabbit IgG HCS | 100 μL |
| KTI2014 | IPKine™ Anti-DDDDK Magnetic IP Kit | 20T |
| KTI2024 | IPKine™ Anti-GFP Magnetic IP Kit | 20T |
| KTI2034 | IPKine™ Anti-GST Magnetic IP Kit | 20T |
| KTI2044 | IPKine™ Anti-HA Magnetic IP Kit | 20T |
| KTI2064 | IPKine™ Anti-Myc Magnetic IP Kit | 20T |
| ABT2013 | Agarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2014 | Magnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2024 | Magnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2023 | Agarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2043 | Agarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2044 | Magnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2053 | Agarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2054 | Magnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2063 | Agarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2064 | Magnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2173 | Agarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2174 | Magnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2010 | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2011 | Anti-DDDDK Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2015 | HRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2020 | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2025 | HRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2021 | Anti-GFP Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2030 | Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2035 | HRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2040 | Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2041 | Anti-HA Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2045 | HRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2050 | Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2051 | Anti-His Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2055 | HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2060 | Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2061 | Anti-Myc Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2065 | HRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2170 | Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 50 μL |
| ABT2070 | Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) | 50 μL |
| ABT2080 | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | 50 μL |
| ABT2090 | Anti-E2 Tag Mouse Monoclonal Antibody (12T4) | 50 μL |
| ABT2100 | Anti-HSV Tag Mouse Monoclonal Antibody (16T2) | 50 μL |
| ABT2110 | Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) | 50 μL |
| ABT2120 | Anti-RFP Tag Mouse Monoclonal Antibody (9D1) | 50 μL |
| ABT2130 | Anti-S Tag Mouse Monoclonal Antibody (9T10) | 50 μL |
| ABT2150 | Anti-T7 Tag Mouse Monoclonal Antibody (6D4) | 50 μL |
| ABT2160 | Anti-Trx Tag Mouse Monoclonal Antibody (14D4) | 50 μL |
| ABT2180 | Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) | 50 μL |
| ABT2190 | Anti-CBP Tag Monoclonal Antibody (12H5) | 50 μL |
| ABT2200 | Anti-TAP Tag Monoclonal Antibody (4H2) | 50 μL |
| ABT2210 | Anti-Avi-Tag Monoclonal Antibody (5G11) | 50 μL |
| ABT2220 | Anti-SRT-Tag Monoclonal Antibody (11G3) | 50 μL |
| ABT2230 | Anti-Strep-Tag II Monoclonal Antibody (8C12) | 50 μL |
| ABT2240 | Anti-mStrawberry Monoclonal Antibody (4C9) | 50 μL |
| ABT2250 | Anti-EYFP-Tag Monoclonal Antibody (10T3) | 50 μL |
| ABT2260 | Anti-mOrange Monoclonal Antibody (9A10) | 50 μL |
| ABT2270 | Anti-AmCyan Monoclonal Antibody (8T2) | 50 μL |
| ABT2280 | Anti-ECFP-Tag Monoclonal Antibody (6B11) | 50 μL |
| ABT2290 | Anti-EBFP Monoclonal Antibody (8B5) | 50 μL |
| ABT2300 | Anti-Nano-Tag9 Monoclonal Antibody (11T3) | 50 μL |