生物素標記試劑盒 | LinKine™ Biotin Labeling Kit Abbkine貨號KTL0120
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生物素標記試劑盒 | LinKine™ Biotin Labeling Kit Abbkine貨號KTL0120
LinKine™ Biotin Labeling Kit
生物素(Biotin)是一種水溶性 B 族維生素(water-soluble B-vitamin)，分子量為 244 Da。生物素(Biotin)與親和素(Avidin)或鏈黴親(Streptavidin)和素具有高親和力，因此生物素(Biotin)-(Streptavidin)系統作為多級信號放大系統廣泛應用於免疫檢測，靈敏度高、特異性強。 LinKine™ Biotin Labeling Kit is designed for preparing Biotin directly from proteins, peptides, and other ligands that contain free amino groups. LinKine™ 生物素標記試劑盒(LinKine™ Biotin Labeling Kit, 貨號KTL0120) 設計用於直接從蛋白質(proteins)、肽(peptides)和其他含有游離氨基( contain free amino groups)的配體製備生物素
Biotin is a water-soluble B-vitamin, with a molecular weight of 244 Da. It is composed of a ureido ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin has high affinity with Avidin or Streptavidin, so the Biotin-Avidin-System is widely used in immune detection as a multistage signal amplification system, with high sensitivity and strong specificity.
圖 1：生物素結構式 LinKine™ 生物素標記試劑盒設計用於直接從蛋白質、肽和其他含有活性氨基的配體製備生物素綴合物。我們試劑盒中提供的生物素已預先激活，可直接用於綴合。 LinKine™ 生物素標記試劑盒包含用於製備生物素標記分子的即用型組件。應用示例：直接標記的一抗具有優勢，因為它們消除了免疫測定程序中對二級試劑的需求，從而消除了 ELISA 和蛋白質印蹟等應用中繁瑣的額外孵育和洗滌步驟。
Materials Supplied and Storage Conditions
List of components (or storage buffer) requirements of the sample to be labeled:
Q1：濃縮後樣本濃度仍低於2 mg/mL？
A1：如果濃縮後樣品濃度仍小於2mg/mL，可以調整標記系統的體積適當，但終濃度應大於1 mg/mL。步驟(1)中生物素標記溶液的體積應為標籤系統體積的 10%。步驟(2)中可以不加入去離子水，其體積為活化的生物素溶液保持不變。您也可以根據自己的實驗進行適當的調整。
Q2：針對不同分子量的待標記樣品，如何選擇適合的純化管柱？
A2：純化管柱適用於分子量為100KD至200KD的樣品。如果樣品帶有分子量高於200KD或低於100KD時，最好再配備更合適的純化尺寸柱子。
Q3：待標記樣品的分子量與生物素相似嗎？
A3：如果樣品的分子量與生物素相似，則完成本說明對應的標記步驟後，加入 30 µL 0.5 µM NH4Cl 並在 37℃ 孵育 10 分鐘淬滅遊離生物素，無需純化步驟。
Q4：不同分子量的分子有不同的偶合效果嗎？
A4：對於分子量小於5KD的分子，偶聯效果較差。您可以增加分子數量適當地。
預染protein marker | 快速型蛋白質染色劑 | 活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色
Abbkine提供一系列高度特異性單克隆和多克隆抗體，tag antibody抗體系列商品如 His tag、GST tag、S tag、HA tag、DDDDK tag、Myc tag、MBP tag和 mCherry tag等單/多株Tag抗體。另具生物素(Biotin)、HRP、AbFluor™ 染料、瓊脂糖(agarose)和磁珠偶聯物(magnetic beads)標誌。
| Cat.No | Name | Size1 |
| KTD104-EN | Universal IP/Co-IP Toolkit (Magnetic Beads) | 20T |
| KTD105-EN | Universal IP/Co-IP Toolkit (Agarose) | 20T |
| A25012 | IPKine™ HRP, Goat Anti-Mouse IgG LCS | 100 μL |
| A25022 | IPKine™ HRP, Mouse Anti-Rabbit IgG LCS | 100 μL |
| A25112 | IPKine™ HRP, Goat Anti-Mouse IgG HCS | 100 μL |
| A25122 | IPKine™ HRP, Mouse Anti-Rabbit IgG HCS | 100 μL |
| A25222 | IPKine™ HRP, Goat Anti-Rabbit IgG HCS | 100 μL |
| KTI2014 | IPKine™ Anti-DDDDK Magnetic IP Kit | 20T |
| KTI2024 | IPKine™ Anti-GFP Magnetic IP Kit | 20T |
| KTI2034 | IPKine™ Anti-GST Magnetic IP Kit | 20T |
| KTI2044 | IPKine™ Anti-HA Magnetic IP Kit | 20T |
| KTI2064 | IPKine™ Anti-Myc Magnetic IP Kit | 20T |
| ABT2013 | Agarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2014 | Magnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2024 | Magnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2023 | Agarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2043 | Agarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2044 | Magnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2053 | Agarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2054 | Magnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2063 | Agarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2064 | Magnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2173 | Agarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2174 | Magnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2010 | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2011 | Anti-DDDDK Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2015 | HRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2020 | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2025 | HRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2021 | Anti-GFP Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2030 | Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2035 | HRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2040 | Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2041 | Anti-HA Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2045 | HRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2050 | Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2051 | Anti-His Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2055 | HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2060 | Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2061 | Anti-Myc Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2065 | HRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2170 | Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 50 μL |
| ABT2070 | Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) | 50 μL |
| ABT2080 | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | 50 μL |
| ABT2090 | Anti-E2 Tag Mouse Monoclonal Antibody (12T4) | 50 μL |
| ABT2100 | Anti-HSV Tag Mouse Monoclonal Antibody (16T2) | 50 μL |
| ABT2110 | Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) | 50 μL |
| ABT2120 | Anti-RFP Tag Mouse Monoclonal Antibody (9D1) | 50 μL |
| ABT2130 | Anti-S Tag Mouse Monoclonal Antibody (9T10) | 50 μL |
| ABT2150 | Anti-T7 Tag Mouse Monoclonal Antibody (6D4) | 50 μL |
| ABT2160 | Anti-Trx Tag Mouse Monoclonal Antibody (14D4) | 50 μL |
| ABT2180 | Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) | 50 μL |
| ABT2190 | Anti-CBP Tag Monoclonal Antibody (12H5) | 50 μL |
| ABT2200 | Anti-TAP Tag Monoclonal Antibody (4H2) | 50 μL |
| ABT2210 | Anti-Avi-Tag Monoclonal Antibody (5G11) | 50 μL |
| ABT2220 | Anti-SRT-Tag Monoclonal Antibody (11G3) | 50 μL |
| ABT2230 | Anti-Strep-Tag II Monoclonal Antibody (8C12) | 50 μL |
| ABT2240 | Anti-mStrawberry Monoclonal Antibody (4C9) | 50 μL |
| ABT2250 | Anti-EYFP-Tag Monoclonal Antibody (10T3) | 50 μL |
| ABT2260 | Anti-mOrange Monoclonal Antibody (9A10) | 50 μL |
| ABT2270 | Anti-AmCyan Monoclonal Antibody (8T2) | 50 μL |
| ABT2280 | Anti-ECFP-Tag Monoclonal Antibody (6B11) | 50 μL |
| ABT2290 | Anti-EBFP Monoclonal Antibody (8B5) | 50 μL |
| ABT2300 | Anti-Nano-Tag9 Monoclonal Antibody (11T3) | 50 μL |