山羊抗Rabbit二級抗體| DyLight 649, Goat Anti-Mouse IgG Abbkine貨號A23620
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山羊抗Rabbit二級抗體| DyLight 647, Goat Anti-Mouse IgG Abbkine貨號A23620 0.5 mg/ml
Dylight 649, Goat Anti-Rabbit IgG
二抗(secondary antibody)是指用於靶向結合一抗的抗體。Abbkine 二抗可與酵素(HRP)、生物素(Biotin)或螢光團偶聯(DyLight)，用於各種基於抗體的應用，包括蛋白質印跡、免疫組織化學、免疫螢光、流式細胞術和 ELISA。，二抗( secondary antibody)和一抗聯合使用，檢測目的蛋白(target protein)，增強檢測信號(detection signal)。我們為您的每種應用提供來自山羊、兔和驢的高質量二抗。也可提供血清吸附二抗，推薦用於富含免疫球蛋白的樣品。Abbkine提供的二抗產品包括常用的酶標二抗（如HRP標記二抗）、DyLight螢光二抗、IFKine™二抗（取自驢，經其他物种血清吸附，具有較低的非-特異性結合和背景。特別適用於免疫學多標記實驗），IPKine™二抗（有效避免重鍊或輕鏈干擾，特別適用於IP-WB檢測）。
產品介紹: 山羊抗Mouse二級抗體，免疫原Mouse IgG whole molecule。可應用於FCM, ICC, IF實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting 1:50-1:1000 dilutions for most fluorescent applications。使用固相小鼠 IgG (H&L) 進行親和純化，最終基於 SDS-PAGE 的純度 > 95%。保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.圖。1。 DyLight 螢光染料是具有更高亮度的新型染料。 DyLight 488 抗體偶聯物比 Cy2 和 FITC 偶聯物更亮，亮度與 Alexa Fluor 488 偶聯物相似。 DyLight 549 抗體偶聯物顯示出比 TRITC 偶聯物更亮的螢光。此外，DyLight 594 抗體偶聯物明顯比 Alexa 594 偶聯物更亮，並且比德克薩斯紅偶聯物更亮且更易溶於水。
Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
圖 2。 DyLight 螢光染料也顯示出改進的光穩定性。 DyLight 488 偶聯物在封片劑中的褪色程度低於 FITC 和 Cy2 偶聯物，這表明 DyLight 488 分子在落射熒光顯微鏡中本身俱有更高的光穩定性。 DyLight 549 偶聯物與 Alexa 555 偶聯物的光穩定性差不多，比 Cy3 偶聯物的光穩定性略高。
Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
圖 3。要將 DyLight Fluors 與螢光成像儀一起使用，請為 DyLight 405 使用藍色激光二極管的光譜線，為 DyLight 488 使用青色 (488 nm) 激光，為 DyLight 550 和 594 使用綠色 (526 nm) 激光，以及紅色 ( 633 nm) 激光用於 DyLight 633 和 650。DyLight 680、755 和 800 螢光與基於激光和濾光片的紅外成像儀器兼容，這些儀器分別在 700 nm、750 nm 和 800 nm 光譜區域發射。
| Product name | DyLight 488, Goat Anti-Mouse IgG |
| Immunogen | Mouse IgG whole molecule |
| Host | Goat |
| Reactivity | Mouse |
| Applications | FCM, ICC, IF |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications. |
| Clonality | Polyclonal |
| Preparation method | Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE |
| Conjugate | Dylight 488 |
| Formulation | Liquid solution |
| Features & Benefits | DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9). |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
預染protein marker | 快速型蛋白質染色劑 | 活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒（β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色
Abbkine提供一系列高度特異性單克隆和多克隆抗體，tag antibody抗體系列商品如 His tag、GST tag、S tag、HA tag、DDDDK tag、Myc tag、MBP tag和 mCherry tag等單/多株Tag抗體。另具生物素(Biotin)、HRP、AbFluor™ 染料、瓊脂糖(agarose)和磁珠偶聯物(magnetic beads)標誌。
| Cat.No | Name | Size1 |
| KTD104-EN | Universal IP/Co-IP Toolkit (Magnetic Beads) | 20T |
| KTD105-EN | Universal IP/Co-IP Toolkit (Agarose) | 20T |
| A25012 | IPKine™ HRP, Goat Anti-Mouse IgG LCS | 100 μL |
| A25022 | IPKine™ HRP, Mouse Anti-Rabbit IgG LCS | 100 μL |
| A25112 | IPKine™ HRP, Goat Anti-Mouse IgG HCS | 100 μL |
| A25122 | IPKine™ HRP, Mouse Anti-Rabbit IgG HCS | 100 μL |
| A25222 | IPKine™ HRP, Goat Anti-Rabbit IgG HCS | 100 μL |
| KTI2014 | IPKine™ Anti-DDDDK Magnetic IP Kit | 20T |
| KTI2024 | IPKine™ Anti-GFP Magnetic IP Kit | 20T |
| KTI2034 | IPKine™ Anti-GST Magnetic IP Kit | 20T |
| KTI2044 | IPKine™ Anti-HA Magnetic IP Kit | 20T |
| KTI2064 | IPKine™ Anti-Myc Magnetic IP Kit | 20T |
| ABT2013 | Agarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2014 | Magnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 1 mL |
| ABT2024 | Magnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2023 | Agarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 1 mL |
| ABT2043 | Agarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2044 | Magnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 1 mL |
| ABT2053 | Agarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2054 | Magnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 1 mL |
| ABT2063 | Agarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2064 | Magnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 1 mL |
| ABT2173 | Agarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2174 | Magnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 1 mL |
| ABT2010 | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2011 | Anti-DDDDK Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2015 | HRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) | 50 μL |
| ABT2020 | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2025 | HRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3) | 50 μL |
| ABT2021 | Anti-GFP Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2030 | Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2035 | HRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8) | 50 μL |
| ABT2040 | Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2041 | Anti-HA Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2045 | HRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6) | 50 μL |
| ABT2050 | Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2051 | Anti-His Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2055 | HRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3) | 50 μL |
| ABT2060 | Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2061 | Anti-Myc Tag Rabbit Polyclonal Antibody | 50 μL |
| ABT2065 | HRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5) | 50 μL |
| ABT2170 | Anti-V5 Tag Mouse Monoclonal Antibody (11D5) | 50 μL |
| ABT2070 | Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) | 50 μL |
| ABT2080 | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) | 50 μL |
| ABT2090 | Anti-E2 Tag Mouse Monoclonal Antibody (12T4) | 50 μL |
| ABT2100 | Anti-HSV Tag Mouse Monoclonal Antibody (16T2) | 50 μL |
| ABT2110 | Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) | 50 μL |
| ABT2120 | Anti-RFP Tag Mouse Monoclonal Antibody (9D1) | 50 μL |
| ABT2130 | Anti-S Tag Mouse Monoclonal Antibody (9T10) | 50 μL |
| ABT2150 | Anti-T7 Tag Mouse Monoclonal Antibody (6D4) | 50 μL |
| ABT2160 | Anti-Trx Tag Mouse Monoclonal Antibody (14D4) | 50 μL |
| ABT2180 | Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) | 50 μL |
| ABT2190 | Anti-CBP Tag Monoclonal Antibody (12H5) | 50 μL |
| ABT2200 | Anti-TAP Tag Monoclonal Antibody (4H2) | 50 μL |
| ABT2210 | Anti-Avi-Tag Monoclonal Antibody (5G11) | 50 μL |
| ABT2220 | Anti-SRT-Tag Monoclonal Antibody (11G3) | 50 μL |
| ABT2230 | Anti-Strep-Tag II Monoclonal Antibody (8C12) | 50 μL |
| ABT2240 | Anti-mStrawberry Monoclonal Antibody (4C9) | 50 μL |
| ABT2250 | Anti-EYFP-Tag Monoclonal Antibody (10T3) | 50 μL |
| ABT2260 | Anti-mOrange Monoclonal Antibody (9A10) | 50 μL |
| ABT2270 | Anti-AmCyan Monoclonal Antibody (8T2) | 50 μL |
| ABT2280 | Anti-ECFP-Tag Monoclonal Antibody (6B11) | 50 μL |
| ABT2290 | Anti-EBFP Monoclonal Antibody (8B5) | 50 μL |
| ABT2300 | Anti-Nano-Tag9 Monoclonal Antibody (11T3) | 50 μL |