TP53 sgRNA CRISPR慢病毒| TP53 sgRNA CRISPR All-in-One Lentivirus set貨號K0005315
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TP53 sgRNA CRISPR慢病毒| 貨號K0005315 3 x 300 µl
TP53 sgRNA CRISPR All-in-One Lentivirus set (Human)
產品介紹: TP53 sgRNA CRISPR All-in-One Lentivirus set (Human)慢病毒, 力價>1×10⁷IU/ml。TP53 (Accession Number NM_000546 ; NM_001276760) 架構於
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro (Human)。Cas9 Promoter啟動子: Cas9核酸酶受SFFV啟動子的控制，除了ES細胞或iPS細胞外，它對絕大多數細胞都有效。 另有EF1a啟動子替換SFFV啟動子作為客製化服務。可直接以非病毒質粒進行轉染用於即時表達。慢病毒包裝系列產品包括Lentivirus慢病毒低速純化(純化) / Lentivirus 慢病毒高濃度純化(純化) 並以 Lentivirus病毒RT-QPCR定量(定量) 純化後之病毒力價。
TP53 sgRNA CRISPR All-in-One Lentivirus set (Human)
| Accession Number | NM_000546 ; NM_001276760 |
| Version | NM_000546 ; NM_001276760 |
| Procedure | |
| Vector | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro |
| Cas9 Promoter | Cas9 Nuclease is under the control of the SFFV promoter which should work for a vast majority of cells, except ES cells or iPS cells. If necessary, the SFFV promoter can be replaced with EF1a promoter as a custom cloning service. Please inquire for pricing. |
| Target Sequence | 23 CCATTGTTCAATATCGTCCG 111 ACCAGCAGCTCCTACACCGG 195 GACGGAAACCGTAGCTGCCC |
| Format | Virus |
| Titer | >1×10⁷IU/ml |
| Appearance | Liquid |
| Storage | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Shelf Life | 1 year (when at -80°C or below in a non-frost free freezer) |
| Shipping | Shipped with dry ice |
| Additional Materials | – CRISPR Genomic Cleavage Detection Kit (G932) – sgRNA CRISPR/Cas9 All-in-One Lentivirus Control (K011) |
| Quality Control | Restriction Enzyme Digest and Sequencing |
慢病毒技術之領導者abm，開發了一套全面的人類、小鼠和大鼠基因文庫，Clone到慢病毒載體或製成即用型慢病毒，可用於操縱感興趣基因於宿主細胞表達。為了促進我們的慢病毒載體的應用和多功能性，ABM慢病毒表達系統具有許多組成型啟動子，標籤序列和報導基因之組合。