【Tag 標籤單多株抗體】Abbkine促銷優惠活動DM&產品資料
【細胞增殖&毒理&衰老】| DNA Damege損傷/細胞Stress | 細胞凋亡&細胞染色 | 發炎反應ELISA分析 | 膽固醇代謝/代謝試驗 | 生化檢測試劑盒 | 抗體蛋白純化&其它 | Tag 標籤單多株抗體
His Tag單株抗體 (5C3) | Abbkine貨號ABT2050
Anti-His Tag Mouse Monoclonal Antibody (5C3)
多組氨酸標籤(polyhistidine-tag)是蛋白質中的氨基酸基序，由至少五個組氨酸 (histidine ,His) 殘基組成，通常位於蛋白質的 N 端或 C 端(C-terminus)。它也被稱為六組氨酸標籤(hexa histidine-tag)、6xHis 標籤(6xHis-tag)和商標名稱 His-tag。多組氨酸標籤(polyhistidine-tagged)通常用於親和純化在大腸桿菌(Escherichia coli)和其他原核表達系統(prokaryotic expression systems)中表達的多組氨酸標籤重組蛋白(polyhistidine-tagged recombinant proteins)。
His Tag單株抗體，免疫原Synthetic Peptide。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於IF, IP, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:5000), IF (1:1500), IP (1:300)。濃度1mg/ml，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.
Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).
Fig.3. IP (1:300) – WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).
| Product name | Anti-His Tag Mouse Monoclonal Antibody (5C3) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Applications | IF, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1500), IP (1:300). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
HA Tag單株抗體 (4F6) | Abbkine貨號ABT2040
Anti-HA Tag Mouse Monoclonal Antibody (4F6)
HA Tag單株抗體，免疫原Synthetic Peptide。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於IF, IP, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:10000), IF (1:2000), IP (1:400)。濃度1mg/ml，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Fig.1. Immunofluorescence staining (1:2000) of HA fusion protein in 293 cells with red and counterstained with DAPI.
Fig.2.IP (1:400)-WB (1:10000) analysis of HA fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with HA-tag protein (lane B); IP untransfected 293 cell lysate with Anti HA tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D), or with Anti HA tag mAb (lane E), and IP transfected 293 without both normal Mouse IgG and HA tag mAb (lane F).
| Product name | Anti-HA Tag Mouse Monoclonal Antibody (4F6) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Applications | IF, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:2000), IP (1:400). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | HA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Background | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
| Alternative | HA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain |
GFP Tag單株抗體 (3D3) | Abbkine貨號ABT2020
Anti-GFP Tag Mouse Monoclonal Antibody (3D3)
綠色螢光蛋白 (green fluorescent protein , GFP) 是一種由 238 個氨基酸殘基 (26.9kD) 組成的蛋白質，當暴露在藍色至紫外線範圍內的光線下時，會呈現出明亮的綠色螢光(green fluorescence)。儘管許多其他海洋生物也有類似的綠色螢光蛋白(green fluorescent proteins)，但 GFP 傳統上指的是首先從水母中分離出來的蛋白質。 GFP 在 395 nm 波長處有一個主要激發峰，在 475 nm 處有一個次要激發峰。其發射峰位於 509 nm，位於可見光譜的較低綠色部分。
GFP Tag單株抗體，免疫原Recombinant Protein。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於IP, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:5000)。濃度1mg/ml，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
| Product name | Anti-GFP Tag Mouse Monoclonal Antibody (3D3) |
| Immunogen | Recombinant Protein |
| Host | Mouse |
| Applications | IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | GFP; Green fluorescent protein |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
mCherry Tag單株抗體 (9D3) | Abbkine貨號ABT2080
Anti-mCherry Tag Mouse Monoclonal Antibody (9D3)
mCherry 是一種螢光團（fluorophore, 一種螢光蛋白），在生物技術中用作跟踪流體流動的示踪劑，在標記分子和細胞成分時作為標記。 mCherry 來源於從 Discosoma sp. (Discosoma sp.)中分離的蛋白質，是一種 28.8kD 單體螢光構建體，在 587 nm 和 610 nm 處具有峰值吸收/發射。 mCherry 有時比其他螢光團更受歡迎，因為它的顏色以及與其他單體螢光團相比的光穩定性。
產品介紹: mCherry Tag單株抗體 (9D3)，免疫原Recombinant Protein。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於IP, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:5000), IF (1:200).。濃度1mg/ml，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Fig.1. Immunofluorescence staining (1:200) of mCherry fusion protein in 293 cells with red.
Fig.2. Western blot analysis of 1ug mCherry fusion protein with Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.
| Product name | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) |
| Immunogen | Recombinant Protein |
| Host | Mouse |
| Applications | IF, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:200). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | mCherry; mCherry tag |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
Myc Tag多株抗體 | Abbkine貨號ABT2061
Anti-Myc Tag Rabbit Polyclonal Antibody
Myc 標籤是一種源自 c-myc 基因產物的多肽蛋白標籤(polypeptide protein tag)，可以使用重組 DNA 技術(recombinant DNA technology)添加到蛋白質中。它可用於親和層析(affinity chromatography)，然後用於將重組的過表達蛋白與宿主生物表達的野生型蛋白分離。它還可用於分離具有多個亞基的蛋白質複合物。
產品介紹: Myc Tag多株抗體，免疫原Synthetic Peptide。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:5000)。濃度50ul，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Fig. Western blot analysis of 1ug Myc fusion protein with Anti-Myc rabbit polyclonal antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.
| Product name | Anti-Myc Tag Rabbit Polyclonal Antibody |
| Immunogen | Synthetic Peptide |
| Host | Rabbit |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen |
| Alternative | Myc tag; Myc Epitope; c-Myc; bHLHe39; myc proto-oncogene protein; oncogene c-Myc |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
S Tag單株抗體(9T10) | Abbkine貨號ABT2130
Anti-S Tag Mouse Monoclonal Antibody (9T10)
S Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins. S-tag 是源自胰腺核糖核酸酶 A (pancreatic ribonuclease A , RNase A) 的寡肽的名稱。原始 RNase A 的 N 端，也稱為 S 肽，由 20 個氨基酸 KETAAAKFERQHMDS 組成。 S Tag 抗體可識別 C 端、內部和 N 端 S 標記蛋白。
產品介紹: S Tag單株抗體(9T10)，免疫原Synthetic Peptide。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:5000)。濃度50ul，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
| Product name | Anti-S Tag Mouse Monoclonal Antibody (9T10) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | S tag; KETAAAKFERQHMDS tag; S peptide; S peptide epitope |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
DDDDK Tag單株抗體(1B10) | Abbkine貨號ABT2010
Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)
DYKDDDDK 肽（Flag-tag）是一種多肽蛋白質標籤(polypeptide protein tag)，可以使用重組 DNA 技術添加到蛋白質中。它可用於親和層析，然後用於將重組的過表達蛋白與宿主生物表達的野生型蛋白分離。它還可用於分離具有多個亞基的蛋白質複合物。
產品介紹: DDDDK Tag單株抗體(1B10)，免疫原Synthetic Peptide。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於IF, IP, WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).。濃度1 mg/ml，保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.
Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).
| Product name | Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Applications | IF, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
GST Tag單株抗體(2A8) | Abbkine貨號ABT2030
Anti-GST Tag Mouse Monoclonal Antibody (2A8)
穀胱甘肽 S-轉移酶 (glutathione S-transferase , GST，以前稱為配體) 酶家族由許多細胞溶質(cytosolic)、線粒體(mitochondrial)和微粒體蛋白(microsomal proteins)組成。 GST 存在於真核生物(eukaryotes)和原核生物(prokaryotes)中，它們催化各種反應並接受內源性(endogenous)和外源性底物(xenobiotic substrates)。 GST 標籤抗體有助於在純化過程中檢測融合蛋白以及從目標蛋白中切割 GST。 GST 標籤抗體具有廣泛的應用，包括您對 GST 蛋白或 GST 融合重組蛋白的研究。
產品介紹: GST Tag單株抗體(2A8)，免疫原Recombinant Protein。通過使用特異性免疫原的親和層析從兔抗血清中親和純化抗體。可應用於WB, IP實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下：Suggested starting dilutions are as follows: WB (1:5000), IP(1:400).。保存於Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Fig. Western blot analysis of GST fusion protein with Anti-GST Mouse Monoclonal Antibody (2A8) in 1:5000 dilutions (lane A) and 1:10000 dilutions (lane A) with 0.5ug GST fusion protein, separately.
| Product name | Anti-GST Tag Mouse Monoclonal Antibody (2A8) |
| Immunogen | Recombinant Protein |
| Host | Mouse |
| Applications | IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IP(1:400). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | GST; Glutathione S Transferase; Glutathione S-transferase class-mu 26 kDa isozyme; Sj26 antigen; sjGST |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| 產品貨號 | 產品品項 | 反應 |
| 貨號ABT2050 | His Tag單株抗體 (5C3) | 50ul |
| 貨號ABT2040 | HA Tag單株抗體 (4F6) | 50ul |
| 貨號 ABT2020 | GFP Tag 單株抗體 (3D3) | 50ul |
| 貨號ABT2080 | mCherry Tag單株抗體(9D3) | 50ul |
| 貨號ABT2061 | Myc Tag多株抗體 | 50ul |
| 貨號ABT2130 | S Tag單株抗體 (9T10) | 50ul |
| 貨號ABT2010 | Anti-DDDDK Tag單株抗體 (1B10) | 50ul |
| 貨號ABT2030 | GST Tag單株抗體 (2A8) | 50ul |
