【血清 Blocking serum】donkey serum/Rabbit serum/Mouse serum/Goat serum

血清(Blocking serum)

封閉血清(Blocking serum)廣泛應用於免疫反應過程中，阻斷(blocking)組織和細胞非特異性抗體結合，而降低非特異性結合所造成的背景值。封閉血清(Blocking serum) 阻斷了樣品中Fc受體(Fc receptors)與實驗中使用的一抗和二抗的結合，並且阻斷了抗體與樣品的非特異性結合(non-specific binding)。通常，用於封閉的血清來自與產生第一抗體的物種不同的物種。封閉性血清通常來自產生第二抗體的物種。可應用於western blot、 ELISA、IHC、Flow分析，降低非特異性抗體結合。血清可直接用於blocking，或作為blocking buffer的成分。常見的封閉血清有正常驢血清(Normal donkey serum)、正常兔血清、(Normal Rabbit serum) 、正常老鼠血清(Normal Mouse serum)、正常山羊血清(Normal Goat serum)。

Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI

Frozen newborn mouse cartilage sections were blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Collagen Type II-UNLB (right) followed by Rabbit F(ab’)2 Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6125-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G® Anti-Fade (SB Cat. No. 0100-35).

血清(Blocking Serum)處理過程以減少溶血情況，並以0.2μm filter過濾。貯存於2-8°C。長期保存-20°C

Cat. No.	Product Description	Source	Size
0030-01	正常驢血清 (Normal Donkey Serum)	Adult donkeys	10 mL
0040-01	正常兔血清 (Normal Rabbit Serum)	Adult New Zealand rabbits	5 mL
0050-01	正常老鼠血清 (Normal Mouse Serum)	Adult non-Swiss albino (NSA) mice	5 mL
0060-01	正常山羊血清 (Normal Goat Serum)	Adult mixed breed goats	10 mL