ApoScreen®7-AAD細胞活力螢光指示劑  | 貨號10042-01 ( CAS# 7240-37-1 )

Synonym: 7-Aminoactinomycin D Ready Made Solution, 7-Aminodactinomycin Ready Made Solution

 

產品介紹: 7-氨基 – 放線菌素D (ApoScreen®7-AAD) 用於活細胞的流式細胞術分析， 7-ADD是細胞活力螢光指示劑 (Fluorescent DNA stain)；Su, X., et al. 該溶液可以與藻紅蛋白（PE）和螢光素（FITC）綴合的抗體一起用於雙色分析。在細胞表面標記物染色後，將細胞在PBS中在冰上用7-AAD進一步染色30分鐘。 PBS洗滌兩次後，將細胞固定在補充有50μg/ ml  actinomycin D 放線菌素D的1％paraformaldehyde多聚甲醛中。非凋亡細胞為7-AAD陰性。 7-AAD與其母體分子Actinomycin D一樣，是具有生長抑制特性的DNA嵌入劑。7-AAD，PE和FITC螢光發射之間的光譜重疊最小， 激發/發射 – 488/647 nm。產品Ready made solution即用型型式 (10 uL/10⁶ cells, final volume of 100 uL )。7-AAD是DNA染料。 它可以用來代替碘化丙啶（PI）來區分死細胞。 這種染料非常適用於長期實驗中的死細胞鑑別，並具有高DNA結合常數。

Description	ApoScreen® 7-AAD is a ready-to-use cell viability dye for the exclusion of nonviable cells in flow cytometry analysis. This solution can be used in conjunction with phycoerythrin (PE) and fluorescein (FITC) conjugated antibodies in 2-color analysis due to minimal spectral overlap between 7-AAD, PE, and FITC fluorescence emissions. Excitation/emission – 488/647 nm
Buffer Formulation	Supplied in PBS/NaN3 and a stabilizing agent
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific SDS
Applications	Flow Cytometry 1-9
Recommended Dilutions	Please refer to product specific Technical Bulletin

References

1. Sjeklocha LM, Park C, Wong PY, Roney MJ, Belcher JD, Kaufman DS, et al. Erythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells. PLoS One. 2011;6(12):e29110. (FC)
2. Ateh DD, Leinster VH, Lambert SR, Shah A, Khan A, Walklin HJ, et al. The intracellular uptake of CD95 modified paclitaxel-loaded poly(lactic-co-glycolic acid) microparticles. Biomaterials. 2011;32:8538-47. (FC)
3. Domm WG 4th. Optimizing antigen delivery approaches for HIV immunization [dissertation]. Rochester (USA): University of Rochester; 2013. (FC)
4. Jenks SA, Palmer E, Marin E, Hartson L, Chida AS, Richardson C, et al. 9G4+ autoantibodies are an important source of apoptotic cell reactivity associated with high levels of disease activity in systemic lupus erythematosus. Arthritis Rheum. 2013;65:3165-75. (FC)
5. Sato EI, Machado LS, Machado AC. The effect of ramipril on endothelial function and endothelial progenitor cells in patients with systemic lupus erythematosus [abstract]. Arthritis Rheum. 2013;65 Suppl 10:584, (FC)
6. Jones MC, Kobie JJ, DeLouise LA. Characterization of cell seeding and specific capture of B cells in microbubble well arrays. Biomed Microdevices. 2013;15:453-63. (FC)
7. De Bruycker M. Invloed van metronomische chemotherapie op myeloid-derived suppressor cellen [thesis]. Ghent (Belgium): Ghent University; 2014 (FC)
8. Dewhurst S, Sullivan MA, inventors; University of Rochester, assignee. Antigenic mimics of discontinuous epitopes of pathogen recognized by broadly neutralizing antibodies. United States patent publication US 8,961,977 B2. 2015 Feb 24. (FC)
9. Andrade AA, da Gama AN, Teixeira GS, Atella GC, de Castro SL, Coutinho CC, et al. Activity of marine sponge-derived extracts against Trypanosoma cruzi is sssociated with apoptosis-like death. Int J Res Stud Biosci. 2015;3(7):92-101. (FC)

ApoScreen® is a registered trademark of SouthernBiotechnology Associates, Inc.

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I