【植物肌動蛋白單株抗體 (3T3)】 Anti-Plant Actin Mouse Monoclonal Antibody 貨號ABL1050
抗植物肌動蛋白小鼠單株抗體 (3T3) | Anti-Plant Actin Mouse Monoclonal Antibody (3T3) Abbkine貨號ABL1050
Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

近日,《Nature》在線發表了華中農業大學李國田教授團隊聯合多家單位完成的題為“Genome Editing of a Rice CDP-DAG Synthase grants multipathogen”的論文。該團隊克隆了廣譜抗病斑點突變基因RBL1,並透過基因編輯創造了新基因RBL1δ12,以增強作物的廣譜抗病性和穩產能力。該基因在作物中高度符合,與傳統抗病基因相比,可以打破物種界限,具有更大的普適性,在抗病育種方面具有巨大的應用潛力。研究成果將使「水稻癌」等疾病的抵抗力提高三個水平,新基因可使水稻產量損失減少近40%!
肌動蛋白(Actin)是一種高度保守的蛋白質,是細胞骨架(cell cytoskeleton)的重要組成部分,在細胞質流動(cytoplasmic streaming)、細胞形狀決定(cell shape determination)、細胞分裂(cell division)、細胞器運動(organelle movement )和延伸生長(extension growth)中發揮重要作用。優先在年輕和擴展的組織、花器官原基( floral organ primordia)、發育中的種子(developing seeds )和新出現的花序中表達。擬南芥(Arabidopsis)有一個複雜的肌動蛋白基因家族,編碼六種蛋白質亞類。其中一類以蛋白質 ACT2 和 ACT8 為代表,它們是相似的蛋白質,但具有不同的側翼序列、內含子和密碼子中的沉默核苷酸位置。 ACT8 在根( root)、莖(stems)、葉(flowers)、花(flowers)、花粉(pollen)和長角果(siliques)中表現。
產品介紹: 抗植物肌動蛋白小鼠單株抗體 (3T3),免疫原Immunogen Recombinant Protein。製備方法 使用特異性免疫原,透過親和層析從小鼠腹水中親和純化抗體。反應物種: Arabidopsis, Brassica napus, Corn。可應用於WB實驗。最佳工作稀釋度應由研究者通過實驗確定。建議的起始稀釋度如下:Suggested starting dilutions are as follows: WB (1:5000-1:10000)。

| Product name | Anti-Plant Actin Mouse Monoclonal Antibody (3T3) |
| Immunogen | Recombinant Protein |
| Host | Mouse |
| Reactivity | Arabidopsis, Brassica napus, Corn |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | AT3G12110; actin-11 |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques. |
| Gene ID | 820385 |
| Alternative | AT3G12110; actin-11 |
| Accession | P53496 |
| Observed Band(KD) | 43 |

https://www.sciencedirect.com/science/article/pii/S2090123222000339

https://www.sciencedirect.com/science/article/pii/S0147651323006711?via%3Dihub

預染protein marker | 快速型蛋白質染色劑 | 活細胞和死細胞雙染色套組 | CCK-8細胞增殖和細胞毒性試劑盒 | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 | Abbkine 細胞膜染色 | 活細胞Lysosome染色
| Cat No. | Product Name | Size |
| ABL1020 | Anti-GAPDH Mouse Monoclonal Antibody (2B5) | 50 μL/200 μL/200 μL×5 |
| ABL1021 | Anti-GAPDH Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABL1030 | Anti-β-Tubulin Mouse Monoclonal Antibody (3G6) | 50 μL/200 μL/200 μL×5 |
| ABL1035 | HRP Conjugated Anti-β-Tubulin Mouse Monoclonal Antibody (3G6) | 50 μL/200 μL/200 μL×5 |
| ABL1010 | Anti-β-Actin Mouse Monoclonal Antibody (1C7) | 50 μL/200 μL/200 μL×5 |
| ABL1011 | Anti-β-Actin Rabbit Polyclonal Antibody | 50 μL/200 μL/200 μL×5 |
| ABL1015 | HRP Conjugated Anti-β-Actin Mouse Monoclonal Antibody (1C7) | 50 μL/200 μL/200 μL×5 |
| ABL1050 | Anti-Plant Actin Mouse Monoclonal Antibody (3T3) | 50 μL/200 μL/200 μL×5 |