蛋白羰基含量呈色測定試劑盒｜Protein carbonyls Assay貨號K830-100 (ABCAM ab126287)

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原廠連結：	http://www.biovision.com
相關下載：	蛋白羰基含量測定試劑盒操作手冊

特點

商品: 蛋白羰基含量測定試劑盒｜貨號K830-100 (ABCAM ab126287)

Simple assay to quantify protein carbonyls

產品介紹: Protein carbonyl groups蛋白質羰基是oxidative stress氧化應激的重要且直接的生物標誌物。蛋白羰基化的DNPH標記一直是氧化應激最常見的措施之一。由反應形成的DNP hydrazones可以在375nm測量。 BioVision的蛋白羰基含量測定試劑盒旨在提供一種簡單而準確的蛋白質樣品中羰基量化方法。使用BSA作為實例，1mg（〜15nmol）樣品具有約0.15nmol羰基的檢測限，其中BSA通常含有約1-3nmol的羰基/ mg

Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375 nm. BioVision’s Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1 mg (~ 15 nmol) sample has a detection limit of about 0.15 nmol carbonyl, where BSA typically contains approximately 1-3 nmol carbonyl/mg.

Size	100 assays
SKU+Size	K830-100 (ABCAM ab126287)
Detection Method	Absorbance ( 375 and 562 nm)
Species Reactivity	Mammalian
Applications	N/A
Features & Benefits	• Simple procedure; takes ~ 30-90 minutes
Kit Components	• DNPH Solution• 100% TCA Solution• 10% Streptozocin Solution• 6 M Guanidine Solution• 96-Well Clear Plate
Storage Conditions	4°C

引用文獻

Somanah, J. et al., (2017) Extracts of Mauritian Carica papaya (var solo) protect SW872 and HepG2 cells against hydrogen peroxide induced oxidative stress, Journal of Food Science and Technology, Apr.2017, 54(7), 1917-1927

Gaspard & McMaster. The Mitochondrial Quality Control Protein Yme1 Is Necessary to Prevent Defective Mitophagy in a Yeast Model of Barth Syndrome. J. Biol. Chem., Apr 2015; 290: 9284 – 9298.

Wilson et al., GCN2 is required to increase fibroblast growth factor 21 and maintain hepatic triglyceride homeostasis during asparaginase treatment.Am J Physiol Endocrinol Metab, Feb 2015; 308: E283 – E293.

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