蛋白羰基含量呈色測定試劑盒｜Protein carbonyls Assay貨號K830-100
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Simple assay to quantify protein carbonyls
產品介紹: Protein carbonyl groups蛋白質羰基是oxidative stress氧化應激的重要且直接的生物標誌物。 蛋白羰基化的DNPH標記一直是氧化應激最常見的措施之一。 由反應形成的DNP hydrazones可以在375nm測量。 BioVision的蛋白羰基含量測定試劑盒旨在提供一種簡單而準確的蛋白質樣品中羰基量化方法。 使用BSA作為實例，1mg（〜15nmol）樣品具有約0.15nmol羰基的檢測限，其中BSA通常含有約1-3nmol的羰基/ mg
Protein carbonyl groups are an important and immediate biomarker of oxidative stress. DNPH tagging of protein carbonyls has been one of the most common measures of oxidative stress. DNP hydrazones formed from the reaction are easily quantifiable at 375 nm. BioVision’s Protein Carbonyl Content Assay Kit is designed to provide a simple and accurate method of quantifying carbonyls in protein samples. Using BSA as an example, a 1 mg (~ 15 nmol) sample has a detection limit of about 0.15 nmol carbonyl, where BSA typically contains approximately 1-3 nmol carbonyl/mg.
| Size | 100 assays |
| SKU+Size | K830-100 |
| Detection Method | Absorbance ( 375 and 562 nm) |
| Species Reactivity | Mammalian |
| Applications | N/A |
| Features & Benefits | • Simple procedure; takes ~ 30-90 minutes |
| Kit Components | • DNPH Solution• 100% TCA Solution• 10% Streptozocin Solution• 6 M Guanidine Solution• 96-Well Clear Plate |
| Storage Conditions | 4°C |
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