Leptin ELISA套組 | Leptin (human) ELISA Kit 貨號K4777 -100

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原廠連結：	http://www.biovision.com
相關下載：	Leptin ELISA套組操作手冊

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商品: Leptin ELISA套組

產品規格: Leptin (human) ELISA Kit 貨號K4777 | 100個分析

產品描述: BioVision的Leptin ELISA（酶聯免疫吸附測定）檢測試劑盒，用於體外定量測定人Leptin之酶聯免疫吸附測定法。該測定方法使用在96孔板上塗覆Leptin之特異性抗體。將標本和樣品移液到孔中，樣品中存在的Leptin通過固定化抗體與孔結合。洗滌孔並加入anti-Leptin-Biotin生物素化的抗Leptin抗體。洗去未結合的anti-Leptin-Biotin生物素化抗體後，將HRP-Steptavidin綴合的鏈黴抗生物素蛋白吸移到孔中。再次洗滌孔，將a ™B substrate solution基質溶液加入到孔中，並且與結合的Leptin的量成比例地顯色。 Stop Solution將顏色從藍色變為黃色，並且在450nm處測量顏色的強度。該ELISA試劑盒顯示與任何測試的細胞因子沒有交叉反應性，例如人血管生成素，BDNF，BLC，ENA-78，FGF-4，IL-1α，IL-1β，IL-2，IL-3，IL-4 ，IL-5，IL-7，IL-8，IL-9，IL-10，IL-11，IL-12 p70，IL-12p40，IL-13，IL-15，IL-309，IP-10 ，G-CSF，GM-CSF，IFN-γ，MCP-1，MCP-2，MCP-3，MDC，MIP-1alpha，MIP-1β，MIP-1gamma，PARC，PDGF，RANTES，SCF，TARC， TGF-β，TIMP-1，TIMP-2，TNF-α，TNF-β，TPO，VEGF。Leptin的最小可檢測劑量通常小於2pg / ml。檢測範圍：2 pg / ml – 400 pg / ml。分析內重現性為CV <10％，分析間CV <12％。

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