脂肪肝測定試劑盒 |TG三酸甘油酯定量+脂滴染色試劑, 貨號 K597-100
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特點
Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O.
肝脂肪變性也稱為脂肪肝，其特徵在於肝臟內脂滴積累過多。 這可能是由多種因素引起的，包括酒精攝入過多，肥胖等脂肪代謝異常的疾病。 肝臟脂肪變性通常用作確定候選藥物的肝毒性的標記物。 BioVision的肝臟脂肪變性試劑盒提供用於測量細胞內甘油三酯積累和染色試劑的試劑，以顯示肝細胞中的脂滴形成。 該試劑盒可用於篩選和評估候選藥物的脂肪變性風險，並研究脂肪變性發展的機制。套組內包括氯喹作為肝細胞誘導脂肪變性的陽性對照，如HepG2。 該試劑盒為三酸甘油酯定量和染色試劑提供足夠的試劑，足夠用於兩個96孔板，兩個6孔板或四個100毫米培養皿。是市場上使用雙重方法來研究肝臟脂肪變性之套組：以（比色/螢光）量化甘油三酯，並通過用油紅O染色來顯現lipid droplets脂滴。
| Cat # +Size | K584-100 |
| Size | 100 assays |
| Detection Method | Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm) and Microscopy |
| Species Reactivity | Mammalian |
| Applications | Evaluate hepatic steatosis, quantify triglycerides, and visualize lipid droplet accumulation Screen drug candidates for steatotic risk |
| Features & Benefits | • Only Kit on the market to use a dual method to study Hepatic Steatosis: Quantifies Triglycerides (colorimetric/fluorometric) and visualizes lipid droplets by staining with Oil Red O. • The kit provides enough reagents for 100 assays for triglyceride quantitation and staining reagents enough for two 96-well plates, two 6-well plates, or four 100 mm culture dishes. • Easy and simple protocol. This kit can be used to screen and evaluate steatosis risk of drug candidates and to study the mechanism of steatosis development. • Chloroquine is included as a positive control for inducing steatosis in liver cells, such as HepG2. |
| Kit Components | • Triglyceride Assay Buffer • Triglyceride Probe (in DMSO) • Lipase • Triglyceride Enzyme Mix • Triglyceride Standard (1 mM) • Chloroquine • PBS • Formalin (10%) • Oil Red O • Methyl Green |
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