商品: PARP Antibody (Clone 10H)單株抗體 | 貨號3000- All species

Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP)

產品介紹: PARP，116kDa核聚（ADP-核糖）聚合酶，是參與DNA修復和細胞凋亡反應的高度保守的核酶。 這種蛋白質可以被許多胱天蛋白酶在體外切割，並且是caspase-3在體內的主要切割靶標之一。 切割發生在ASP214和Gly 215之間，其將PARP的N末端DNA結合結構域（24kDa）與其C末端催化結構域（89kDa）分離。 已經表明，PARP的切割促進細胞解聚和抑制PARP切割減弱體外凋亡。

Size	100 µg
SKU+Size	3000-100
Antibody Target	PARP
Alternate Name	PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
Host	Mouse
Antibody Type	Monoclonal
Isotype	Mouse IgG3κ
Accession #	NP_001609
Gene ID	142
Appearance	Colorless liquid
Concentration	1.0 mg/ml
Formulation	1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.
Purification	Chromatography
Species Reactivity	All species
Application	Western blot, Immunoprecipitation, Immunocytochemistry & ELISA
Positive Control	Tested by ICC in rat liver tissues
Application & Usage	Immunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods; <2 μg/ml for ECL); Immunoprecipitation and ELISA
Handling	The antibody solution should be gently mixed before use.

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