Human Type IV Collagen-Solution | 貨號 1250-01S (0.5 mg/mL) 1ml包裝
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Human Type IV Collagen-Solution | 貨號 1250-01S (0.5 mg/mL) 1ml包裝
細胞免疫專家極力推薦SouthernBiotech高品質膠原– Collagen
關於膠原（Collagen）：
膠原蛋白(Collagen)是動物結締組織重要的蛋白質,在哺乳動物中的含量尤為豐富，佔有機體蛋白質百分之25%-35%含量。膠原是細胞外最重要的水不溶性纖維蛋白, 是構成細胞外基質（extracellular matrix, ECM）的骨架。膠原在細胞外基質中形成半晶體的纖維,給細胞提供抗張力和彈性,並在細胞的遷移和發育中起作用。膠原蛋白的基本結構單位是原膠原(tropocollagen), 原膠原肽鏈的一級結構具有(Gly-x-y)n重複序列, 其中x常為脯氨酸(Pro), y常為羥脯氨酸(Hypro)或羥賴氨酸(Hylys)。Hylys殘基可發生糖基化修飾,其糖單位有的是一個半乳糖殘基(Gal), 但通常是二糖(Glu-Gal-),膠原上的糖所占的量約為膠原的10%。原膠原是由三條α-肽鏈組成的纖維狀蛋白質, 相互擰成三股螺旋狀構型, 長300nm, 直徑1.5nm。目前已發現29種不同組織中不同類型的膠原; 不同類型的膠原定位於體內的特定組織,也有2-3種不同的膠原存在於同一組織中。而機體內90%的膠原為I、II、III、IV型。
產品描述：膠原蛋白(Collagen)是細胞外空間中的主要結構蛋白，是ECM中最豐富的蛋白。膠原蛋白分為兩類-原纖維（I，II，III，V型）和非原纖維（IV，VI型）。 IV型膠原蛋白主要在基底膜(basal lamina)中表達，因此構成了基底膜(basement membrane)的一部分。 IV型膠原蛋白突變與Alport綜合徵相關，而α3（IV） 鏈被認為是Goodpasture綜合徵中的抗原。 IV型膠原由六個命名為α1（IV）至α6（IV）的異構鏈組成。SouthernBiotech提供一系列高品質膠原- Collagen , 其中Human Type IV Collagen-Solution (貨號1250-01S )來源為Placental villi, 純化方式經由控制和限制胃蛋白酶消化，然後進行選擇性鹽沉澱。純度> 90% by SDS-PAGE，貯存在500 mM acetic acid溶液中，濃度為0.5mg/ml, 體積1ml。
| Description | Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes – fibril (types I, II, III, V) and non-fibril (types IV, VI). Type IV collagen is primarily expressed in the basal lamina and therefore constitutes a portion of the basement membrane. Type IV collagen mutations are associated with Alport syndrome and the α3(IV) chain is thought to be the antigen implicated in Goodpasture’s syndrome. Type IV collagen is comprised of six isomeric chains designated α1(IV) to α6(IV). |
| Source | Placental villi |
| Purity | > 90% by SDS-PAGE |
| Purification Method | Controlled and limited pepsin digestion followed by selective salt precipitation |
| Buffer Formulation | 500 mM acetic acid |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | ELISA – Quality tested 2-4 SDS-PAGE – Quality tested 1 Coating Material for – Adhesion Studies – Reported in literature 5,6 Blood Disorder Studies – Reported in literature 2-4 Differentiation Studies – Reported in literature 7 ECM Interaction Studies – Reported in literature 8,9 |
ELISA plate was coated with serially diluted Mouse Type IV Collagen (SB Cat. No. 1250-04S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
膠原蛋白
| 產品名稱 | 編號（cat#） | 包裝形式 | 規格 |
| Type I Human Collagen | 1200-01S | 溶液 | 0.50mg |
| Type I Bovine Collagen | 1200-02/1200-02S | 凍乾粉/溶液 | 0.25mg/0.50mg |
| Type I Rat Collagen | 1200-03/1200-03S | 凍乾粉/溶液 | 0.25mg/0.50mg |
| Type II Rat Collagen | 1220-02/1220-02S | 凍乾粉/溶液 | 0.25mg/0.50mg |
| Type III Human Collagen | 1230-01S | 溶液 | 0.50mg |
| Type III Bovine Collagen | 1240-02S | 溶液 | 0.50mg |
| Type IV Human Collagen | 1250-01S | 溶液 | 0.50mg |
| Type IV Mouse Collagen | 1250-04S | 溶液 | 0.50mg |
| Type IV Bovine Collagen | 1260-02 | 凍乾粉 | 0.25mg |
| Type V Human Collagen | 1270-01/1270-01S | 凍乾粉/溶液 | 0.1mg/0.25mg |
| Type V Bovine Collagen | 1280-02S | 溶液 | 0.25mg |
| Type VI Human Collagen | 1290-01S | 溶液 | 0.25mg |
| Type VI Bovine Collagen | 1300-02/1300-02S | 凍乾粉/溶液 | 0.10mg/0.25mg |
膠原蛋白多株抗體
| 產品名稱 | 編號（cat#） | 包裝形式 | 規格 |
| Goat Anti-Type I Collagen | 1310-01/1310-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type II Collagen | 1320-01/1320-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type III Collagen | 1330-01/1330-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type IV Collagen | 1340-01/1340-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type V Collagen | 1350-01/1350-08 | 未標記/生物素化 | 0.2mg/0.2mg |
| Goat Anti-Type VI Collagen | 1360-01/1360-08 | 未標記/生物素化 | 0.2mg/0.2mg |
膠原蛋白單株抗體
| 產品名稱 | 編號（cat#） | 包裝形式 | 規格 |
| Mouse Anti-Human Type I Collagen | 1440(1)-01 | 未標記 | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-01 | 未標記 | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-02 | FITC | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-08 | BIOT | 0.1mg |
| Mouse Anti-Human Type IV Collagen | 1460-09 | R-PE | 0.1mg |
應用： ELISA、免疫螢光、免疫細胞化學、免疫組織化學（冰凍切片）
Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |
References
- Hou L, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia. Acta Biomater. 2016;44:188-99. (Microarray, Cell Culture)
- Slobodianuk TL, Kochelek C, Foeckler J, Kalloway S, Weiler H, Flood VH. Defective collagen binding and increased bleeding in a murine model of von Willebrand disease affecting collagen IV binding. J Thromb Haemost. 2019;17:63-71. (ELISA, Coating, Blood Disorder Studies, Adhesion Studies, Aggregation Studies)