永生化人類心臟纖維母細胞 | ABMGOOD貨號T0446
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Abmgood不朽系列商品 (immortalized cell)-永生化人類心臟纖維母細胞
Immortalized Human Cardiac Fibroblasts
永生化人類心臟纖維母細胞特性
細胞型態: Adherent, multipolar。 來源Heart。解凍後，細胞需要4-5天才能恢復培養。 細胞培養條件: 使用PriCoat™T25培養瓶 (貨號G299)，以最佳化初代和不朽細胞的生長、增殖和擴增。或塗層細胞外基質(Applied Cell Extracellular Matrix) 增強細胞貼附 (貨號G422)。Grow cells in ECM-coated culture vessels with the following conditions. 培養液PriGrow IV (貨號TM004)。+ 10% FBS + 1 mM NEAA (TM068) + 2 mM L-glutamine (G275)+ 5 µg/ml insulin (TM053) + 1% Penicillin/Streptomycin Solution (G255) 二氧化碳（CO2）：5％，溫度：37.0℃。
| Cat. No. | T0446 |
| Name | Immortalized Human Cardiac Fibroblasts |
| Description | Human Cardiac Fibroblast is an important heart component that provides structural support for cardiomyoctes. They synthesize extracellular matrix, growth factors and cytokines during growth. In addition, they also interact with myocardial responses to injury and pathophysiological conditions such as scar formation at sites of myocardial infarction, cardiac fibrosis and cardiac hypertrophy. These cells are ideal models for study in cardiac matrix response modeled by physiological and pathological stressors. Real Time PCR was used to quantify the SV-40 transgene expression. |
| Organism | Human (H. sapiens) |
| Tissue | Heart |
| Donor History | Not disclosed, Ventricle |
| Growth Properties | Adherent, multipolar |
| Cell Type | Immortalized Cells |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions | PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1 mM NEAA (TM068) + 2 mM L-glutamine (G275)+ 5 µg/ml insulin (TM053) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. |
| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
| Thawing Protocol |
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. 5. Incubate the cells at the recommended conditions. |
| Subculture Protocol |
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent. 1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment. 3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type. 5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. 6. Incubate the cells at the recommended conditions. |
| Cryopreservation | Cryopreservation Medium (TM024), or complete growth media with 10% DMSO. |
| Seeding Density (cells/cm2) | 40,000 – 50,000 |
| Population Doubling Time (h) | 48 – 58 |
| Immortalization Method | Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen |
| Expression | vWF and caveolin-1 |
HOT!! 黴漿菌PCR偵測 | DAPI染核 | 細胞內黴漿菌去除劑 | 水浴槽抑菌劑 | 噴霧式去黴劑 等系列商品
Abmgood不朽化系列商品 (immortalized reagenet) Ready-to-use
Abmgood公司於人類與老鼠不朽之細胞株上，具有幾十年的實驗經驗；提供一系列不朽 (immortalized)化之細胞株、及不朽化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus，可直接加於細胞中進行感染表現；病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液；亦提供一系列病毒力價濃縮與定量試劑商品。
細胞不朽化的工具中，SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因（p53、Rb等）的不活化相關，會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞，SV40 T anbtigen會誘導端粒酶（Telomerase）的活性。與端粒（telomere）長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中，hTERT是處於不活化態，必要時會進行活化保持telomere的長度，避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現（over-expression）對於誘發細胞不朽化是無效的（如初代上皮細胞），反而會造成細胞死亡；同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。
Abmgood不朽化系列商品 (immortalized cell)
根據細胞類型，Primary cells初代細胞在體外具有小於10-50次傳代的有限壽命。 不朽化細胞株能夠延長增殖，並且具有與親代組織相似或相同的基因型和表現型。
哺乳動物細胞的不朽化Immortalized策略
存在用於在培養物中不朽化哺乳動物細胞的幾種方法。 Abmgood多年研發經驗，開發出了一系列Immortalized商品；包括即用型逆轉錄病毒，慢病毒和腺病毒載體。
| Gene Overexpressed | Viruses Offered | Application |
| hTERT | Adenovirus, Lentivirus, Retrovirus |
可靠和常用。 可能在一些細胞類型（例如上皮細胞）中引起細胞死亡 |
| SV40 T Antigen | Adenovirus, Lentivirus, Retrovirus |
可靠和常用。 通常用於不朽化上皮細胞。 |
| EBV genes | Epstein-Barr Virus | 不朽化B淋巴細胞，有時是T淋巴細胞。 |
| HPV E6-E7 | Lentivirus | 不朽化角化細胞。 |
| HOXB8, HOXA9, HOXA10 | Lentivirus | 不朽化造血細胞，包括巨噬細胞，造血祖細胞和骨髓祖細胞。 |
| Adenoviral E1A-E1B | Lentivirus | 不朽化大範圍的初級囓齒動物細胞，包括羊水細胞，原發性上皮細胞，成纖維細胞和原代腎細胞。 |
| p53, Rb siRNA | Lentivirus | 與hTERT共表達以更好地使上皮細胞不朽化。 |
| Bmi1 | Lentivirus | 不朽化上皮細胞。 |
| CDK4 | Lentivirus | 與hTERT共同表達更真實的細胞模型。 |
| Myc T58A (c-Myc), Ras V12 (H-Ras) | Lentivirus | 不朽化各種細胞，包括神經幹細胞，初代腎細胞，成紅細胞和各種上皮細胞（原發性前列腺，乳腺，氣道等）。 |
Decomplement加熱非活化FBS：
1.讓FBS瓶在4°C下完全解凍過夜。
2.將FBS瓶置於56°C的水浴中30分鐘。
Decomplemented FBS可以在-20°C下長期保存。避免頻繁的凍融循環。每2-3天更換一次完整的媒體。不要讓介質顏色變為黃色。
Thawing細胞解凍方法：(每種細胞建議條件可能有許不同，依照原廠建議操作，以下為通用方式) 操作方式下載
1.在37℃水浴中解凍小瓶，直到只有一小塊冰塊。(DMSO對細胞有毒性，over-thaw過度解凍下可能造Stress)
2.將細胞轉移到含有5ml pre-warmed complete media 之15ml管中。
3.將細胞以290xg離心5分鐘。
4.小心丟棄上清液，不要打擾細胞沉澱，輕輕地上下移液，將細胞重新懸浮在1ml完全培養基中。
5.將細胞培養在50,000 – 100,000 cells/cm2。 cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5
DMSO在細胞凍存中的應用
二甲基亞碸是一種重要的滲透型細胞保護劑。在深低溫（零下200度）保存細胞時凍存過程中，為防止細胞內液冰晶形成、滲透壓改變、細胞結構紊亂等導致的損傷，有必要使用含有DMSO冷凍保護劑。DMSO能夠快速穿透細胞膜進入細胞中，降低冰點、延緩凍存過程，同時提高細胞內離子濃度，減少細胞內冰晶的形成，從而減少細胞損傷。深低溫時二甲基亞碸的細胞毒性受到抑制。復蘇時動作要快，儘快洗掉二甲基亞碸，否則會造成對細胞嚴重的毒性。二甲基亞碸（DMSO）是目前最好的細胞凍存保護劑，但也是一種以細胞毒性很大的化學試驗劑。研究結果表明，培養液中DMSO濃度為10%時，細胞生長抑制率近100%；1‰濃度時抑制率為35%，即使是0.04‰的濃度，DMSO對細胞的生長也有不利的影響。
Subculture細胞繼代方法: (每種細胞建議條件可能有許不同，依照原廠建議操作，以下為通用方式) 操作方式下載
1.當細胞70-90％ confluency時，建議繼代培養。
2. Aspirate old media。懸浮液中的一些細胞仍然是活細胞。或者，也可以收集含有懸浮細胞的上清液並離心（Skip to Step 6 in protocol）。
3.加入1：1比例的1X無菌PBS和0.25％胰蛋白酶-EDTA（TM051）。
4.在室溫下孵育細胞3-5分鐘並攪拌培養容器直至90％的細胞脫落。
5. 中和胰蛋白酶:添加等量之trypsin + PBS。
6.收集細胞並以290xg離心5分鐘。
7.丟棄上清液，輕輕地上下移液，將細胞重新懸浮在完全培養基中。
8.建議split ratio比不超過1：3。
Freezing細胞凍結：(每種細胞建議條件可能有許不同，依照原廠建議操作，以下為通用方式) 操作方式下載
Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.
儲存溫度：液氮氣相。重要提示：此細胞係對FBS批次非常敏感。我們強烈建議最終用戶使用從PAN biotech或Nucleus Biologics 之FBS。
慢病毒包裝系列產品Lentivirus慢病毒低速純化(純化) | Lentivirus 慢病毒高濃度純化(純化) | Lentivirus病毒RT-QPCR定量(定量)
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| T0697 | Immortalized Human Cranial Suture Progenitor Cells (iSuPs) |
| T0723 | Immortalized Human Foreskin Keratinocytes (HFK-16E6E7) |
| T0727 | Immortalized Human Prostate Stromal Cells (PM151T) – hTERT |
| T0752 | Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) |
| T0764 | Immortalized Human Endometriotic Cell Line (12Z) |
| T0841 | Immortalized Human Dental Pulp Stem Cells (TP-023) |
| T0842 | Immortalized Human Dental Pulp Stem Cells (TP-037) |
| T1013 | Immortalized Human Diploid Fibroblasts – hTERT |
| T1015 | Immortalized Human Lung Fibroblast – hTERT |
| T9435 | Immortalized Human Cancer Associated Fibroblast Cells – SV40 |
| T3000 | Acan CRISPR Knockout RCS Cell Line (Rat) |
| T3002 | Aquaporin-4 Stable 293 Cell Line |
| T3005 | MuSK Stable HEK293 Cell Line |
| T3006 | Luciferase Stable PC3 Cell Line |
| T3008 | Luciferase (Luc2) Stable TRAMP Cell Line |
| T3009 | Luciferase Stable U87MG/ΔEGFR Cell Line |
| T3013 | Luciferase Stable WM-266-4 Cell Line |
| T3015 | NF-kB Luciferase Stable RAW264.7 Cell Line |
| T3016 | HEK293-CymR-Puro Stable Cell Line |
| T3017 | A2058-CymR Stable Cell Line |
| T3018 | SKMEL5-CymR Stable Cell Line |
| T3021 | GRPR-Luciferase Stable PC3 Cell Line |
| T3024 | Bax Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3025 | Bak Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3026 | Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3027 | Atg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3028 | Atg7 Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3030 | Nav2.1/β1/Contactin Stable Chinese Hamster Lung Cell Line |
| T3031 | PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line |
| T3032 | Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line |
| T3033 | Dual Notch1(Variant)-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1G4<sup>esn</sup>) Cell Line |
| T3034 | TLR3 Knockout Immortalized Mouse Dendritic Cell Line |
| T3035 | TLR9 Knockout Immortalized Mouse Dendritic Cell Line |
| T3036 | Type I IFN Receptor Knockout Immortalized Mouse Dendritic Cell Line |
| T3037 | Ryanodine Receptor 1 Stable HEK293 Cell Line |
| T3038 | KCC2 Stable HEK293 Cell Line |
| T3039 | Stable HEK293 Expressing Mutant UQCRB-MT1 Cell Line |
| T3040 | c-Kit/D816V Stable Ba/F3 Cell Line |
| T3041 | c-Kit/WT Stable Ba/F3 (with GNNK- isoform) Cell Line |
| T3042 | c-Kit/WT Stable Ba/F3 (with GNNK+ isoform) Cell Line |
| T3043 | c-Kit/Y568F Stable Ba/F3 Cell Line |
| T3044 | c-Kit/Y570F Stable Ba/F3 Cell Line |
| T3045 | c-Kit Y568F/Y570F Stable Ba/F3 Cell Line |
| T3046 | c-Kit/Y703F Stable Ba/F3 Cell Line |
| T3047 | c-Kit/Y936F Stable Ba/F3 Cell Line |
| T3048 | c-Kit Y703F/Y936F Stable Ba/F3 Cell Line |
| T3049 | c-Kit/N705AStable Ba/F3 Cell Line |
| T3050 | c-Kit/N938A Stable Ba/F3 Cell Line |
| T3051 | c-Kit N705A/N938A Stable Ba/F3 Cell Line |
| T3052 | c-Kit/D816H Stable Ba/F3 Cell Line |
| T3053 | c-Kit/D816F Stable Ba/F3 Cell Line |
| T3054 | c-Kit/D816YStable Ba/F3 Cell Line |
| T3055 | c-Kit/V560D Stable Ba/F3 Cell Line |
| T3056 | c-Kit/K642E Stable Ba/F3 Cell Line |
| T3057 | c-Kit/Y721F Stable Ba/F3 Cell Line |
| T3058 | c-Kit/Y823F Stable Ba/F3 Cell Line |
| T3059 | TLR2 Stable HEK293 Cell Line |
| T3060 | PGC-1α/ERRα Stable 293T Cell Line |
| T3061 | TNK1 Stable Ba/F3 Cell Line |
| T3062 | ALK Stable Ba/F3 Cell Line |
| T3063 | LTK Stable Ba/F3 Cell Line |
| T3064 | AXL Stable Ba/F3 Cell Line |
| T3065 | MERTK Stable Ba/F3 Cell Line |
| T3066 | TYRO3 Stable Ba/F3 Cell Line |
| T3067 | EPHA1 Stable Ba/F3 Cell Line |
| T3068 | EPHA5 Stable Ba/F3 Cell Line |
| T3069 | EPHA7 Stable Ba/F3 Cell Line |
| T3070 | EPHA8 Stable Ba/F3 Cell Line |
| T3071 | FGFR2 Stable Ba/F3 Cell Line |
| T3072 | FGFR3 Stable Ba/F3 Cell Line |
| T3073 | IGF1R Stable Ba/F3 Cell Line |
| T3074 | INSR Stable Ba/F3 Cell Line |
| T3075 | JAK1 Stable Ba/F3 Cell Line |
| T3076 | JAK2 Stable Ba/F3 Cell Line |
| T3077 | JAK3 Stable Ba/F3 Cell Line |
| T3078 | MET Stable Ba/F3 Cell Line |
| T3079 | MST1R Stable Ba/F3 Cell Line |
| T3080 | FLT3 Stable Ba/F3 Cell Line |
| T3081 | KIT Stable Ba/F3 Cell Line |
| T3082 | PDGFRA Stable Ba/F3 Cell Line |
| T3083 | PDGFRB Stable Ba/F3 Cell Line |
| T3084 | RET Stable Ba/F3 Cell Line |
| T3085 | ROS1 Stable Ba/F3 Cell Line |
| T3086 | BLK Stable Ba/F3 Cell Line |
| T3087 | FGR Stable Ba/F3 Cell Line |
| T3088 | FYN Stable Ba/F3 Cell Line |
| T3089 | HCK Stable Ba/F3 Cell Line |
| T3090 | LCK Stable Ba/F3 Cell Line |
| T3091 | SRC Stable Ba/F3 Cell Line |
| T3092 | YES1 Stable Ba/F3 Cell Line |
| T3093 | SYK Stable Ba/F3 Cell Line |
| T3094 | NTRK1 Stable Ba/F3 Cell Line |
| T3095 | NTRK2 Stable Ba/F3 Cell Line |
| T3096 | NTRK3 Stable Ba/F3 Cell Line |
| T3097 | FLT4 Stable Ba/F3 Cell Line |
| T3098 | ERBB2 Stable Ba/F3 Cell Line |
| T3099 | EPHA4 Stable Ba/F3 Cell Line |
| T3100 | ABL1 Stable Ba/F3 Cell Line |
| T3101 | ABL2 Stable Ba/F3 Cell Line |
| T3102 | AZ-AHR Stable HepG2 Luciferase Reporter Cell Line |
| T3103 | AZ-GR Stable HeLa Luciferase Reporter Cell Line |
| T3104 | AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line |
| T3105 | BMP Responsive Immortalized Reporter (BRITER) Cell Line |
| T3106 | EGFR Stable Ba/F3 Cell Line |
| T3107 | ERBB4 Stable Ba/F3 Cell Line |
| T3108 | EPHA2 Stable Ba/F3 Cell Line |
| T3109 | EPHB1 Stable Ba/F3 Cell Line |
| T3110 | EPHB2 Stable Ba/F3 Cell Line |
| T3111 | TYK2 Stable Ba/F3 Cell Line |
| T3112 | EPHB4 Stable Ba/F3 Cell Line |
| T3113 | FER Stable Ba/F3 Cell Line |
| T3115 | LYN Stable Ba/F3 Cell Line |
| T3116 | ZAP70 Stable Ba/F3 Cell Line |
| T3117 | TIE1 Stable Ba/F3 Cell Line |
| T3118 | KDR Stable Ba/F3 Cell Line (IL-3 Independent) |
| T3119 | TNK2 Stable Ba/F3 Cell Line |
| T3120 | CSK Stable Ba/F3 Cell Line |
| T3121 | MATK Stable Ba/F3 Cell Line |
| T3122 | DDR1 Stable Ba/F3 Cell Line |
| T3123 | DDR2 Stable Ba/F3 Cell Line |
| T3124 | PTK2 Stable Ba/F3 Cell Line |
| T3126 | EPHA10 Stable Ba/F3 Cell Line |
| T3127 | EPHA3 Stable Ba/F3 Cell Line |
| T3128 | EPHA6 Stable Ba/F3 Cell Line |
| T3129 | EPHB3 Stable Ba/F3 Cell Line |
| T3131 | PTK2B Stable Ba/F3 Cell Line |
| T3132 | FGFR4 Stable Ba/F3 Cell Line |
| T3133 | INSRR Stable Ba/F3 Cell Line |
| T3134 | AATK Stable Ba/F3 Cell Line |
| T3136 | LMTK3 Stable Ba/F3 Cell Line |
| T3137 | MuSK Stable Ba/F3 Cell Line |
| T3138 | CSF1R Stable Ba/F3 Cell Line |
| T3139 | PTK7 Stable Ba/F3 Cell Line |
| T3140 | ROR1 Stable Ba/F3 Cell Line |
| T3141 | ROR2 Stable Ba/F3 Cell Line |
| T3142 | RYK Stable Ba/F3 Cell Line |
| T3143 | FRK Stable Ba/F3 Cell Line |
| T3145 | SRMS Stable Ba/F3 Cell Line |
| T3146 | BMX Stable Ba/F3 Cell Line |
| T3147 | BTK Stable Ba/F3 Cell Line |
| T3148 | ITK Stable Ba/F3 Cell Line |
| T3149 | TEC Stable Ba/F3 Cell Line |
| T3150 | TXK Stable Ba/F3 Cell Line |
| T3151 | TEK2 Stable Ba/F3 Cell Line |
| T3152 | FLT1 Stable Ba/F3 Cell Line |
| T3153 | STYK1 Stable Ba/F3 Cell Line |
| T3154 | SPINK1 Stable AsPC-1 Cell Line |
| T3155 | hSSAO/VAP-1 A7r5 Stable Cell Line |
| T3156 | Mouse R-spondin1 Stable 293T Cell Line |
| T3157 | Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line |
| T3158 | TrkA Stable T17 Cell Line |
| T3159 | TrkB Stable T48 Cell Line |
| T3160 | Luc-Akt-PH Stable MCF7 Cell Line |
| T3161 | Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line |
| T3162 | HEK293S-TetR Stably Expressing TP Cell Line |
| T3163 | HEK293S-TetR GnTI- Stably Expressing TP Cell Line |
| T3164 | HEK293S-TetR Stably Expressing A160T Cell Line |
| T3165 | HEK293S-TetR GnTI- Stably Expressing A160T Cell Line |
| T3166 | Cx26 Stable BxPC-3 Cell Line |
| T3167 | E-cadherin Stable MIA PaCa-2 Cell Line |
| T3168 | BMP9 Stable HEK293 Cell Line |
| T3169 | 92 TAg Stable Y-LIZ Wildtype Mouse Embryonic Fibroblast Cell Line |
| T3170 | 88 Tag Stable Y-LIZ DNA Polymerase-β Knockout Mouse Embryonic Fibroblast Cell Line |
| T3171 | SIRT1 Wiltype Stable RKO Cell Line |
| T3172 | SIRT1 Knockout Stable RKO Cell Line |
| T3173 | SIRT1 Wildtype Stable DLD-1 Cell Line |
| T3174 | SIRT1 Knockout Stable DLD-1 Cell Line |
| T3175 | SIRT1 Wildtype Stable 293T Cell Line |
| T3176 | SIRT1 Knockout Stable 293T Cell Line |
| T3177 | PZ-TR Stable HepG2 Luciferase Reporter Cell Line |
| T3178 | Ad5 pTP Stable 293 (293pTP) Cell Line |
| T3181 | p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line |
| T3182 | Podoplanin Stably Expressing CHO Cell Line |
| T3183 | Stable Retinal Pigment Epithelial Expressing Truncated hELG1-CFP Cell Line |
| T3184 | Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line |
| T3185 | Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line |
| T3186 | Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line |
| T3187 | Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line |
| T3188 | Stable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line |
| T3189 | Stable VERO Overexpressing HER2 Cell Line |
| T3190 | Stable VERO Overexpressing HER3 Cell Line |
| T3191 | Stable VERO Overexpressing HER2 and HER3 Cell Line |
| T3192 | Stable VERO Overexpressing HER1 Cell Line |
| T3193 | Stable CHO Overexpressing HER2 Cell Line |
| T3194 | Stable VERO Overexpressing HER1 and HER2 Cell Line |
| T3195 | Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line |
| T3196 | Stable HA-Tagged hGALE Expressing CHO Cell Line |
| T3197 | Stable G90E Mutated hGALE Expressing CHO Cell Line |
| T3198 | Stable V94M Mutated hGALE Expressing CHO Cell Line |
| T3199 | Stable C307Y Mutated hGALE Expressing CHO Cell Line |
| T3203 | CRE Expressing 293 Cell Line |
| T3207 | ICP47 Stable Raji Cell Line |
| T3208 | ICP47 Stable Mel JuSo Cell Line |
| T3209 | HLA-DQ8 Expressing Stable K562 Cell Line |
| T3210 | Mutant AKT1 Stable Proteus Syndrome Derived Cell Line (F6B) |
| T3217 | GFP Stably Expressing Schwann cells |
| T3218 | huGRPr Expessing Stable Ace-1 Cell Line |
| T3219 | Apolipoprotein A-1 Expressing Stable MC38 Cell Line (MC38 Apo) |
| T3220 | Apolipoprotein A-1 and P144 Over-expressing Stable MC38 Cell Line (MC38 ApoLinkerP144) |
| T3222 | BRCA1 L1780P Mutant Stable HCC1937 Cell Line |
| T3223 | BRCA1 L1780P Mutant Stable HCC1395 TNBC Cell Line |
| T3224 | NDUFS2 Stable Knockout HEK293 Cell Line (HEK293ΔNDUFS2) |
| T3229 | HEK293-T Cells Stably Expressing MLL-AF6 (MA6) |
| T3230 | HEK293-T Cells Stably Expressing AF6-MLL (A6M) |
| T3231 | HEK293-T Cells Stably Expressing MLL-AF6/AF6-MLL (CO1) |
| T3232 | HEK293-T Cells Stably Expressing exMLL-AF6 (exMA6) |
| T3233 | HEK293-T Cells Stably Expressing AF6-shMLL (A6shM) |
| T3234 | HEK293-T Cells Stably Expressing exMLL-AF6/AF6-shMLL (CO2) |
| T3235 | A549 Cells Stably Expressing hACE2 Receptor and TMPRSS2 (A549-AT) |
| T3236 | U2OS Stably Expressing NONO-APEX2 Cell Line (U2OS:NONO-APEX2-HA) |
| T3241 | ts-46 Stable Knockout HEK293 Cell Line (ts-46 293KO) |
| T3242 | ts-101 Stable Knockout HEK293 Cell Line (ts-101 293KO) |
| T3243 | Human Progerin Expressing 3T3-L1 Stable Cell Line (3T3L1-PG) |