尿素測定試劑盒(Urea Assay Kit) | ABNOVA貨號KA1652
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尿素測定試劑盒(Urea Assay Kit) | ABNOVA貨號KA1652
線性檢測範圍 0.08 mg/dL (13 μM) 至 100 mg/dL (17 mM)
尿素(Urea)主要在肝臟( liver )中產生在血液中溶解(dissolved)和運輸(transported)（濃度為1.6-7.5mM），並由腎臟排泄 (kidney)。尿素(Urea)是蛋白質的主要最終產物動物的分解代謝。它是從體內清除有毒氨( toxic ammonia )的主要載體。尿素(Urea)測定對於臨床醫生評估患者的腎功能非常有用。一般來說，尿素(Urea)水平升高與腎炎(nephritis)、腎缺血(renal ischemia)、尿路梗阻(urinary tract obstruction)和某些腎外疾病有關，例如充血性心力衰竭、肝臟疾病和糖尿病。尿素(Urea)水平降低表明急性肝功能不全(acute hepatic insufficiency )或可能是由過度強力的腸外液體治療引起的。用於測量尿素(Urea)濃度或血尿素氮 BUN 的簡單、直接且自動化的程序生物樣品中的成分在研究和藥物發現中變得越來越流行。尿素測定試劑盒(Urea Assay Kit, KA1652)旨在直接測量生物樣品中的尿素(Urea)，無需任何預處理。改進的榮格方法( Jung method)利用顯色試劑，專門與尿素(urea)形成有色複合物。測量的顏色強度在 520 nm 處，與樣品中的尿素(urea)濃度成正比。優化配方大大減少了原始樣品中物質的干擾。尿素測定試劑盒(Urea Assay Kit)直接測定：血清、血漿、尿液、乳汁、細胞/組織培養物、支氣管肺泡灌洗液 (BAL) 等中的尿素。可應用於藥物發現/藥理學：藥物對尿素代謝的影響。環境：廢水、土壤等中尿素測定。尿素測定試劑盒(Urea Assay Kit, KA1652)靈敏度高、準確。使用 5 μL 樣品。線性檢測範圍 0.08 mg/dL (13 μM) 至 100 mg/dL (17 mM) 。
Specification
- Product Description:
- Urea Assay Kit is a quantitative colorimetric urea determination.
- Suitable Sample:
- Bronchoalveolar Lavage (BAL), Cell/Tissue Culture, Milk, Plasma, Serum, Urine and Environmental Samples (waste water, soil etc.)
- Sample Volume:
- For 96-well Plate: 5 uL [Serum and plasma samples can be assayed directly (n = 1). Urine samples should be diluted 50-fold in distilled water prior to assay (n = 50).] or 50 uL [For low urea samples (< 5 mg/dL), e.g. tissue/cell extract, BAL etc or for culture medium containing phenol red]
For Cuvettes: 20 uL or for low urea samples, use 200 uL instead of 20 uL.
- Detection Method:
- Colorimetric
- Assay Type:
- Quantitative
- Calibration Range:
- 0.08 to 100 mg/dL (13 uM to 17 mM)
- Limit of Detection:
- 0.08 mg/dL (13 uM)
- Regulation Status:
- For research use only (RUO)
- Quality Control Testing:
- Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
- Storage Instruction:
- Store the kit at -20°C. For best results, store the components as described in the protocol.
Avoid repeated freezing and thawing.
Publication Reference
1.Preoperative Function Assessment of Ex Vivo Kidneys with Supervised Machine Learning Based on Blood and Urine Markers Measured during Normothermic Machine Perfusion.
Wenke Markgraf, Hagen Malberg.Biomedicines. 2022 Nov 28;10(12):3055. doi: 10.3390/biomedicines10123055.
2.Setup of human liver-chips integrating 3D models, microwells and a standardized microfluidic platform as proof-of-concept study to support drug evaluation.
Benoit Cox, Patrick Barton, Reiner Class, Hannah Coxhead, Claude Delatour, Eric Gillent, Jamie Henshall, Emre M Isin, Lloyd King, Jean-Pierre Valentin.Biomater Biosyst. 2022 May 30;7:100054. doi: 10.1016/j.bbiosy.2022.100054. eCollection 2022 Aug.
3.Microdosing as a Potential Tool to Enhance Clinical Development of Novel Antibiotics: A Tissue and Plasma PK Feasibility Study with Ciprofloxacin.
Zoe Oesterreicher, Sabine Eberl, Beatrix Wulkersdorfer, Peter Matzneller, Claudia Eder, Esther van Duijn, Wouter H. J. Vaes, Birgit Reiter, Thomas Stimpfl, Walter Jäger, Alina Nussbaumer‑Proell, Daniela Marhofer, Peter Marhofer, Oliver Langer, Markus Zeitlinger.Clin Pharmacokinet. 2022 May;61(5):697-707. doi: 10.1007/s40262-021-01091-1. Epub 2022 Jan 7.
4.Enhanced ammonia detoxification to urea in hepatocytes transduced with human aquaporin-8 gene.
Alejo M Capiglioni, Gabriela L Muller, Julieta Marrone, Maria de Lujan Alvarez, Raúl A Marinelli.Biotechnol Bioeng. 2021 Nov;118(11):4331-4337. doi: 10.1002/bit.27901. Epub 2021 Jul 28.
5.Inhibition of neprilysin with sacubitril without RAS blockage aggravates renal disease in Dahl SS rats.
Iuliia Polina, Morgan J Spicer, Mark Domondon, Ryan S Schibalski, Elizaveta Sarsenova, Regina F Sultanova, Daria V Ilatovskaya.Ren Fail. 2021 Dec;43(1):315-324. doi: 10.1080/0886022X.2021.1879856.
6.Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system.
Hafiz Muhammad Umer Farooqi, Bohye Kang, Muhammad Asad Ullah Khalid, Abdul Rahim Chethikkattuveli Salih, Kinam Hyun, Sung Hyuk Park, Dongeun Huh, Kyung Hyun Choi.Nano Converg. 2021 Feb 2;8(1):3. doi: 10.1186/s40580-021-00253-y.
7.Is It Possible to Store Spotted Wolffish (Anarhichas Minor) Sperm by Refrigeration?
W A González-López, D M Patel, N Duncan, J Beirão.Fish Physiol Biochem. 2021 Jun;47(3):725-735. doi: 10.1007/s10695-020-00820-w. Epub 2020 Jun 2.
8.Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely From Induced Pluripotent Stem Cells.
Kazuki Takeishi, Alexandra Collin de l’Hortet, Yang Wang, Kan Handa, Jorge Guzman-Lepe, Kentaro Matsubara, Kazutoyo Morita, Sae Jang, Nils Haep, Rodrigo M Florentino, Fangchao Yuan, Ken Fukumitsu, Kimimasa Tobita, Wendell Sun, Jonathan Franks, Evan R Delgado, Erik M Shapiro, Nicolas A Fraunhoffer, Andrew W Duncan, Hiroshi Yagi, Tomoji Mashimo, Ira J Fox, Alejandro Soto-Gutierrez.Cell Rep. 2020 Jun 2;31(9):107711. doi: 10.1016/j.celrep.2020.107711.
9.The Differential Effects of Low Dose Sacubitril and/or Valsartan on Renal Disease in Salt-Sensitive Hypertension.
Iuliia Polina, Mark Domondon, Rebecca Fox, Anastasia Sudarikova, Miguel Troncoso, Valeriia Vasileva, Yuliia Kashyrina, Monika Beck Gooz, Ryan Schibalski, Kristine Y DeLeon-Pennell, Wayne R Fitzgibbon, Daria V Ilatovskaya.Am J Physiol Renal Physiol. 2020 Jul 1;319(1):F63-F75. doi: 10.1152/ajprenal.00125.2020. Epub 2020 May 28.
10.Data of ureagenesis from ammonia, glutamine and alanine, and mitochondrial aquaporin-8 expression in thioacetamide-treated hepatocytes.
Alejo M Capiglioni, María de Luján Alvarez, Raúl A Marinelli.Data Brief. 2020 Apr 30;30:105632. doi: 10.1016/j.dib.2020.105632. eCollection 2020 Jun.
11.Innate immunity ascertained from blood and tracheal aspirates of preterm newborn provides new clues for assessing bronchopulmonary dysplasia.
Zaramella P, Munari F, Stocchero M, Molon B, Nardo D, Priante E, Tosato F, Bonadies L, Viola A, Baraldi E.PLoS One. 2019 Sep 4;14(9):e0221206. doi: 10.1371/journal.pone.0221206. eCollection 2019.
12.In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity.
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13.Differentiation of human-induced pluripotent stem cell under flow conditions to mature hepatocytes for liver tissue engineering.
Starokozhko V, Hemmingsen M, Larsen L, Mohanty S, Merema M, Pimentel RC, Wolff A, Emnéus J, Aspegren A, Groothuis G, Dufva M.J Tissue Eng Regen Med. 2018 May;12(5):1273-1284. doi: 10.1002/term.2659. Epub 2018 Apr 6.
14.Characteristics of Infection Immunity Regulated by Toxoplasma gondii to Maintain Chronic Infection in the Brain.
Hwang YS, Shin JH, Yang JP, Jung BK, Lee SH, Shin EH.Front Immunol. 2018 Feb 5;9:158. doi: 10.3389/fimmu.2018.00158. eCollection 2018.
15.Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes.
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16.Macrophage PTEN Regulates Expression and Secretion of Arginase I Modulating Innate and Adaptive Immune Responses.
Sahin E, Haubenwallner S, Kuttke M, Kollmann I, Halfmann A, Dohnal AB, Chen L, Cheng P, Hoesel B, Einwallner E, Brunner J, Kral JB, Schrottmaier WC, Thell K, Saferding V, Bluml S, Schabbauer GJ Immunol. 2014 Aug 15;193(4):1717-27. doi: 10.4049/jimmunol.1302167. Epub 2014 Jul 11.
17.Circulating adipocyte fatty acid binding protein is increased in chronic and acute renal dysfunction.
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18.Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism.
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