ALDH2 ELISA試劑盒| Human Aldehyde dehydrogenase (ALDH) ELISA Kit貨號KTE62505

ALDH2屬於蛋白質的醛脫氫酶家族(aldehyde dehydrogenase family)。醛脫氫酶(Aldehyde dehydrogenase)是酒精代謝的主要氧化途徑(alcohol metabolism)中的第二種酶。醛脫氫酶(aldehyde dehydrogenase)的兩種主要肝臟同工型(liver isoforms)，胞質(cytosolic)和線粒體(mitochondrial)，可以通過其電泳遷移率(electrophoretic mobilities)，動力學(kinetic properties)特性和亞細胞定位(subcellular localizations)來區分。大多數高加索(Caucasians)人有兩種主要的同工酶，而大約50％的東亞人(East Asians)具有胞漿同工酶(cytosolic isozyme)，但沒有線粒體同工酶(mitochondrial isozyme)。東亞人中急性酒精中毒的頻率比白種人中高得多，這可能與線粒體同工酶(mitochondrial isozyme)缺乏催化活性形式有關。具有催化失活形式的個體對乙醛的暴露增加也可能賦予許多類型的癌症更大的敏感性。該基因編碼線粒體同工型(mitochondrial isoform)，其對於乙醛的Km低，位於線粒體基質(mitochondrial matrix)中。選擇性剪接導致編碼不同同工型的多個轉錄變體。

產品描述：人醛脫氫酶（Human Aldehyde dehydrogenase , ALDH）ELISA試劑盒採用兩點夾心ELISA(Sandwich ELISA)定量樣品中的ALDH2。對ALDH2特異的抗體已預先包被在微孔板上。將標準品和樣品吸取到孔中，並且存在的任何ALDH2都被固定的抗體結合。除去所有未結合的物質後，將HRP-綴合ALDH2檢測抗體添加到孔中。洗滌以除去任何未結合的HRP試劑後，將色原溶液添加到孔中，並且根據在初始步驟中結合的ALDH2的量成比例地顯色。停止顯色並測量顏色的強度。可應用於以下樣品來源: Cell culture supernatants, Other biological fluids, Plasma, Serum

• Product name

Human Aldehyde dehydrogenase (ALDH) ELISA Kit

• Reactivity

Human

• Applications

ELISA

• Detection method

Colorimetric

• Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

• Assay type

Sandwich ELISA (quantitative)

• Assay duration

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

• Kit components
• Human Aldehyde dehydrogenase microplate
  • Human Aldehyde dehydrogenase standard
  • Human Aldehyde dehydrogenase detect antibody
  • Streptavidin-HRP
  • Standard diluent
  • Assay buffer
  • HRP substrate
  • Stop solution
  • Wash buffer
  • Plate covers
• Features & Benefits

Human Aldehyde dehydrogenase (ALDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALDH2. No significant cross-reactivity or interference between Human ALDH2 and analogues was observed.

• Calibration range

Please inquire

• Limit of detection

Please inquire

• Usage notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
  • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
  • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
  • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
  • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
  • It is recommended that all samples and standards be assayed in duplicate or triplicate.

Alternative names

ALDH2; ALDH-E2; ALDHI; ALDM; MGC1806; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; mitochondrial aldehyde dehydrogenase 2; nucleus-encoded mitochondrial aldehyde dehydrogenase 2

 

使用注意事項

• 請勿混用不同試劑盒中的組分，或在試劑盒到期日期之後使用試劑。
• 打開之前，讓所有試劑至少加室溫30分鐘。
• 用試劑預清洗移液器吸頭，為每個樣品，標準液和試劑使用新鮮的移液器吸頭，以免污染。
• 未使用的孔必須在提供的密封袋中於4°C乾燥。
• 徹底混合對於結果非常重要。建議每10分鐘使用低頻振盪器或稍稍搖動手。
• 建議所有樣品和標準品一式兩份或一式三份進行測定。