Mouse Anti-Human IgG4 Fc-HRP抗體 | Southernbiotech貨號9200-05

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相關下載：	Mouse Anti-Human IgG4 Fc-HRP抗體操作手冊

特點

Mouse Anti-Human IgG4 Fc-HRP抗體 | 貨號9200-05 規格1.0 mL
美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:
二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。
產品介紹: Mouse Anti-Human IgG4 Fc-HRP抗體，具HRP標誌。針對Human IgG4 Fc具特異性。免疫原為Human IgG4 myeloma protein。
Isotype為Mouse (BALB/c) IgG1κ。Isotype ControlMouse IgG1-HRP。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG4 Fc-HRP (SB Cat. No. 9200-05).

Clone	HP6025
Isotype	Mouse (BALB/c) IgG₁κ
Isotype Control	Mouse IgG₁-HRP
Immunogen	Human IgG₄ myeloma protein
Specificity	Human IgG₄ Fc
Format/Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – ELISA – Quality tested ^2-9 FLISA – Quality tested Flow Cytometry – Reported in literature ^15,18,19 Immunohistochemistry-Frozen Sections – Reported in literature ¹⁰ Immunohistochemistry-Paraffin Sections – Reported in literature ^5,7,10,11 Immunocytochemistry – Reported in literature ¹² Western Blot – Reported in literature ^3,13-16 Microarray – Reported in literature ¹⁷ Multiplex – Reported in literature ^2,20-22 Purification – Reported in literature ¹⁵
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796691

Gene ID	3503 (Human)
Gene Symbol	IGHG4 (Human)
Uniprot ID	P01861 (Human)
Uniprot Name	IGHG4_HUMAN

引用文獻

References

Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen)
Arnold M, Dechant M, Doxiadis II, Spriewald BM. Prevalence and specificity of immunoglobulin G and immunoglobulin A non-complement-binding anti-HLA alloantibodies in retransplant candidates. Tissue Antigens. 2008;72:60-6. (ELISA, Multiplex)
Bogdanos D, Pusl T, Rust C, Vergani D, Beuers U. Primary biliary cirrhosis following Lactobacillus vaccination for recurrent vaginitis. J Hepatol. 2008;49:466-73. (ELISA, WB)
Forthal DN, Landucci G, Ding H, Kappes JC, Wang A, Thung I, et al. IgG2 inhibits HIV-1 internalization by monocytes, and IgG subclass binding is affected by gp120 glycosylation. AIDS. 2011;25:2099-104. (ELISA)
Jia X, Hu S, Chen J, Qu Z, Liu G, Cui Z, et al. The clinical and immunological features of patients with combined anti-glomerular basement membrane disease and membranous nephropathy. Kidney Int. 2014;85:945-52. (ELISA, IHC-PS)
Xu J, Wang L, Deng C, Hu X, Li R, Chen T, et al. The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection. Parasitol Res. 2014;113:149-55. (ELISA)
Zhang J, Zhao L, Gao Y, Liu M, Li T, Huang Y, et al. A classification of Hashimoto’s thyroiditis based on immunohistochemistry for IgG4 and IgG. Thyroid. 2014;24:364-70. (ELISA, IHC-PS)
Westra J, van Assen S, Wilting KR, Land J, Horst G, de Haan A, et al. Rituximab impairs immunoglobulin (Ig)M and IgG (subclass) responses after influenza vaccination in rheumatoid arthritis patients. Clin Exp Immunol. 2014;178:40-7. (ELISA)
Seitz-Polski B, Payré C, Ambrosetti D, Albano L, Cassuto-Viguier E, Berguignat M, et al. Prediction of membranous nephropathy recurrence after transplantation by monitoring of anti-PLA2R1 (M-type phospholipase A2 receptor) autoantibodies: a case series of 15 patients. Nephrol Dial Transplant. 2014;29:2334-42. (ELISA)
Detlefsen S, Bräsen JH, Zamboni G, Capelli P, Klöppel G. Deposition of complement C3c, immunoglobulin (Ig)G4 and IgG at the basement membrane of pancreatic ducts and acini in autoimmune pancreatitis. Histopathology. 2010;57:825-35. (IHC-FS, IHC-PS)
Qu Z, Liu G, Li J, Wu L, Tan Y, Zheng X, et al. Absence of glomerular IgG4 deposition in patients with membranous nephropathy may indicate malignancy. Nephrol Dial Transplant. 2012;27:1931-7. (IHC-PS)
de Bruin A, Caldelari R, Williamson L, Suter MM, Hunziker T, Wyder M, et al. Plakoglobin-dependent disruption of the desmosomal plaque in pemphigus vulgaris. Exp Dermatol. 2007;16:468-75. (ICC)
Müller E, Kernland K, Caldelari R, Wyder M, Balmer V, Hunziker T, et al. Unusual pemphigus phenotype in the presence of a Dsg1 and Dsg3 autoantibody profile. J Invest Dermatol. 2002;118:551-5. (WB)
Bogdanos D, Pares A, Baum H, Caballeria L, Rigopoulou EI, Ma Y, et al. Disease-specific cross-reactivity between mimicking peptides of heat shock protein of Mycobacterium gordonae and dominant epitope of E2 subunit of pyruvate dehydrogenase is common in Spanish but not British patients with primary biliary cirrhosis. J Autoimmun. 2004;22:353-62. (WB)
Keen J, Serghides L, Ayi K, Patel SN, Ayisi J, van Eijk A, et al. HIV impairs opsonic phagocytic clearance of pregnancy-associated malaria parasites. PLoS Med. 2007;4(5):e181. (WB, FC, Purificaton)
Parcina M, Miranda-Garcia MA, Durlanik S, Ziegler S, Over B, Georg P, et al. Pathogen-triggered activation of plasmacytoid dendritic cells induces IL-10-producing B cells in response to Staphylococcus aureus. J Immunol. 2013;190:1591-602. (WB)
Savilahti EM, Rantanen V, Lin JS, Karinen S, Saarinen KM, Goldis M, et al. Early recovery from cow’s milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow’s milk epitopes. J Allergy Clin Immunol. 2010;125:1315-21. (Microarray)
Johansson SE, Hejdeman B, Hinkula J, Johansson MH, Romagné F, Wahren B, et al. NK cell activation by KIR-binding antibody 1-7F9 and response to HIV-infected autologous cells in viremic and controller HIV-infected patients. Clin Immunol. 2010;134:158-68. (FC)
Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014;14:1581-91. (FC)
Lowe D, Higgins R, Zehnder D, Briggs DC. Significant IgG subclass heterogeneity in HLA-specific antibodies: Implications for pathogenicity, prognosis, and the rejection response. Hum Immunol. 2013;74:666-72. (Multiplex)
Arnold M, Ntokou I, Doxiadis II, Spriewald BM, Boletis JN, Iniotaki AG. Donor-specific HLA antibodies: evaluating the risk for graft loss in renal transplant recipients with isotype switch from complement fixing IgG1/IgG3 to noncomplement fixing IgG2/IgG4 anti-HLA alloantibodies. Transpl Int. 2014;27:253-61. (Multiplex)
Schaub S, Hönger G, Koller MT, Liwski R, Amico P. Determinants of C1q binding in the single antigen bead assay. Transplantation. 2014;98:387-93. (Multiplex)

Clone	JDC-10
Isotype	Mouse IgG₁κ
Isotype Control	Mouse IgG₁-HRP
Immunogen	Purified human IgG₁ myeloma protein
Specificity	Human/Rhesus/Chimpanzee IgG Fc
Format/Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	Supplied in a stock solution in 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume	1.0 mL
Storage & Handling	Store at 2-8°C Please refer to product specific (M)SDS
Applications	Applications for relevant formats of this clone include – ELISA – Quality tested ^1-15,20 FLISA – Quality tested Flow Cytometry – Quality tested ^18,19 Immunohistochemistry-Paraffin Sections – Reported in literature ¹⁶ Immunoblotting – Reported in literature ¹⁷ Multiplex – Reported in literature ^20-22
Recommended Dilutions	Please refer to product specific Technical Bulletin

SouthernBiotech antibodies are used in laboratories around the world, accelerating the advancement of SARS-CoV-2 serological assays, vaccine development, and COVID-19 research by our global network of collaborators. Our in-house manufacturing capabilities ensure long-term viability of assays and products once released to the market.

Summary of immunoglobulin classes and subclasses –
Immunoglobulin Classes – IgG, IgM, IgA, IgD, IgE
Mouse Immunoglobulin Subclasses – IgG₁, IgG_2a/IgG_2c, IgG_2b, IgG₃
Human Immunoglobulin Subclasses – IgG₁, IgG₂, IgG₃, IgG₄, IgA₁, IgA₂
Rat Immunoglobulin Subclasses – IgG₁, IgG_2a, IgG_2b, IgG_2c
Hamster Immunoglobulin Subclasses – IgG₁, IgG₂, IgG₃
Light Chains – Kappa, Lambda
Heavy Chains – IgG (gamma/γ), IgM (mu/μ), IgA (alpha/α), IgD (delta/δ), IgE (epsilon/ε)

Cat. No.	Product Description	Clone	Size
2053-01	Goat Anti-Human IgA, Mouse/Bovine/Horse SP ads-UNLB	Polyclonal	1.0 mg
9020-05	Mouse Anti-Human IgM-HRP	SA-DA4	1.0 mL
9020-01	Mouse Anti-Human IgM-UNLB	SA-DA4	0.5 mg
9130-05	Mouse Anti-Human IgA₁-HRP	B3506B4	1.0 mL
9130-01	Mouse Anti-Human IgA₁-UNLB	B3506B4	0.5 mg
9140-05	Mouse Anti-Human IgA₂-HRP	A9604D2	1.0 mL
9140-01	Mouse Anti-Human IgA₂-UNLB	A9604D2	0.5 mg
2014-05	Goat Anti-Human IgG Fc, Multi-Species SP ads-HRP	Polyclonal	1.0 mL
2014-01	Goat Anti-Human IgG Fc, Multi-Species SP ads-UNLB	Polyclonal	0.5 mg
9040-05	Mouse Anti-Human IgG Fc-HRP	JDC-10	1.0 mL
9040-01	Mouse Anti-Human IgG Fc-UNLB	JDC-10	0.5 mg
9042-05	Mouse Anti-Human IgG Fc-HRP	H2	1.0 mL
9042-01	Mouse Anti-Human IgG Fc-UNLB	H2	0.5 mg

Conjugates偶聯物的選擇取決於應用和檢測技術（比色法、化學發光法或螢光法）。對於蛋白質印跡和 ELISA 應用，通常使用酶偶聯二抗，例如辣根過氧化物酶 (HRP) 和鹼性磷酸酶 (AP)，並且都可以使用比色和化學發光平台進行檢測。對於流式細胞術或免疫螢應用，需要與螢染料偶聯的二抗（例如，Alexa Fluor®、FITC、TRITC、APC、PE）。


Cat. No.	Product Description	Clone	Size
2053-01	Goat Anti-Human IgA, Mouse/Bovine/Horse SP ads-UNLB	Polyclonal	1.0 mg
9020-05	Mouse Anti-Human IgM-HRP	SA-DA4	1.0 mL
9020-01	Mouse Anti-Human IgM-UNLB	SA-DA4	0.5 mg
9130-05	Mouse Anti-Human IgA₁-HRP	B3506B4	1.0 mL
9130-01	Mouse Anti-Human IgA₁-UNLB	B3506B4	0.5 mg
9140-05	Mouse Anti-Human IgA₂-HRP	A9604D2	1.0 mL
9140-01	Mouse Anti-Human IgA₂-UNLB	A9604D2	0.5 mg
2014-05	Goat Anti-Human IgG Fc, Multi-Species SP ads-HRP	Polyclonal	1.0 mL
2014-01	Goat Anti-Human IgG Fc, Multi-Species SP ads-UNLB	Polyclonal	0.5 mg
9040-05	Mouse Anti-Human IgG Fc-HRP	JDC-10	1.0 mL
9040-01	Mouse Anti-Human IgG Fc-UNLB	JDC-10	0.5 mg
9042-05	Mouse Anti-Human IgG Fc-HRP	H2	1.0 mL
9042-01	Mouse Anti-Human IgG Fc-UNLB	H2	0.5 mg

Max D. Cooper

Emory University School of Medicine

美國抗體生產商Southern Biotech於1982年，由阿拉巴馬大學伯明翰分校 UAB拉斯克獎得主細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。在過去的 37 年中，SouthernBiotech 已將 Cooper 實驗室產生的眾多雜交瘤商業化，包括幾種禽類特異性抗體，其模型有助於開發適應性免疫的核心原理。致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

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