LGR5 Lentiviral Vector (Human) |AMBGOOD 貨號264910610395
特點
LGR5 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)慢病毒表現載體 | 貨號264910610395
產品描述: LGR5 Lentiviral Vector (Human) (BC096324) 架構於pLenti-GIII-CMV-GFP-2A-Puro表現載體上，具有GFP報導基因，構成LGR5 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)。慢病毒載體，可結合packaging mix (改良式 病毒包裝系統 LV003) 以在多種宿主細胞或動物模型中過表達目標miRNA。 該構建體含有用於監測轉染效率的GFP報告基因(GFP reporter )，可以Monitor轉染效率。可直接以非病毒質粒轉染 (non-viral plasmid transfection) 立即表達目標蛋白，或經由包裝成慢病毒顆粒，實現高效轉導和穩定整合的表達目標蛋白。
| Product Name | LGR5 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
|---|---|
| Accession Number | BC096324 |
| Description | The LGR5 expression will be driven by a CMV promoter, with a GFP reporter. Inserts are flanked by and can be excised using NheI and BamHI as long as inserts do not contain any internal NheI or BamHI sites. |
| Cat.No | 264910610395 |
| Gene Name | LGR5 |
| Full Gene Name | leucine-rich repeat-containing G protein-coupled receptor 5 |
| Aliases | HG38, FEX |
| List of species | Human |
| Units | 1.0 µg DNA |
| Vector | pLenti-GIII-CMV-GFP-2A-Puro |
| Applications |
|
| System | Lentiviral Vector |
| Insert Size | 2724bp |
| List of Markers | Bacterial: Kanamycin. Mammalian: Puromycin |
| Promoters | CMV |
| Tag | GFP |
| Appearance | Clear Liquid |
| Vector Size | 8829bp |
| Format | Lentiviral Plasmid |
| Storage Buffer | 10mM Tris-HCI, 1mM EDTA, pH8.0 |
| Storage | 1 year when stored at -20°C or lower in a non-frost free freezer. |
| Reconstitution | 5′-NheI and 3′-BamHI |
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