Mouse Anti-Chicken MHC Class II-PE抗體 | 貨號8350-09 規格1.0 mg  (1.0 mg/mL)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質一級及二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

雞主要組織相容性複合物(chicken major histocompatibility complex , MHC),或B複合體(B complex),由幾個高度多態基因簇(highly polymorphic genes)組成。與哺乳動物一樣,鳥類MHC對免疫反應(immune response)、抗病(disease resistance)、生長髮育(growth and development)、衰老(aging)和繁殖(reproduction)等各種重要的生物功能具有遺傳影響。雞MHC II類基因,也稱為B-L分區域(B-L subregion),雞MHC編碼細胞表面糖蛋白(chicken MHC encode cell surface glycoproteins)是哺乳動物II類抗原的同源。B-L抗原在結構上與哺乳動物II類分子相似,因為它們是一個重鏈和一條輕鏈的非共價結合的暗角。MHC II 類主要表現在 B 細胞和抗原呈現細胞 (antigen presenting cells , APC)。

產品介紹:  Mouse Anti-Chicken MHC Class II-PE抗體，具PE (R-phycoerythrin)標誌。針對Chicken/Pigeon/Caiman MHC class II β-chain具特異性。來源為Swine抗血清。Isotype為Swine IgG。Isotype Control Goat IgG-FITC。 緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑及stabilizer。

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken MHC Class II-PE (SB Cat. No. 8350-09) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).

Clone	2G11
Isotype	Mouse IgG1κ
Isotype Control	Mouse IgG1-PE
Specificity	Chicken/Pigeon/Caiman MHC class II β-chain
Alternalte Name(s)	B-L
Format/Conjugate	PE (R-phycoerythrin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.1 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 1,4,6-19 Immunohistochemistry-Frozen Sections – Reported in literature 1,4,20 Electron Microscopy – Reported in literature 5 Immunoprecipitation – Reported in literature 1,2 Purification – Reported in literature 3
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796515

 

Gene ID	693256 (Chicken) 101747454 (Chicken)
Gene Symbol	BLB1 (Chicken) BLB2 (Chicken)
Gene Aliases	B-LB; B-LB21; GSP-BLB1; B-L; B-LB2; B-LBII; GSP-BLB2; S19

 

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

引用文獻

1.Salomonsen J, Dunon D, Skjødt K, Thorpe D, Vainio O, Kaufman J. Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system. Proc Natl Acad Sci USA. 1991;88:1359-63. (IP, IHC-FS, FC)

2.Kaufman J, Skjoedt K, Salomonsen J, Simonsen M, Du Pasquier L, Parisot R, et al. MHC-like molecules in some nonmammalian vertebrates can be detected by some cross-reactive xenoantisera. J Immunol. 1990;144:2258-72. (IP, Caiman Reactivity)

3.Salomonsen J, Marston D, Avila D, Bumstead N, Johansson B, Juul-Madsen H, et al. The properties of the single chicken MHC classical class II α chain (B-LA) gene indicate an ancient origin for the DR/E-like isotype of class II molecules. Immunogenetics. 2003;55:605-14. (Purification)

4.Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS, FC)

5.del Cacho E, Gallego M, Lee SH, Lillehoj HS, Quilez J, Lillehoj EP, et al. Induction of protective immunity against Eimeria tenella infection using antigen-loaded dendritic cells (DC) and DC-derived exosomes. Vaccine. 2011;29:3818-25. (EM)

6.Ekino S, Riwar B, Kroese FG, Schwander EH, Koch G, Nieuwenhuis P. Role of environmental antigen in the development of IgG+ cells in the bursa of fabricius. J Immunol. 1995;155:4551-8. (FC)

7.Katevuo K, Vainio O. Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery. Immunology. 1996;89:419-23. (FC)

8.Madruga J, Koritschoner NP, Diebold SS, Kurz SM, Zenke M. Polarised expression pattern of focal contact proteins in highly motile antigen presenting dendritic cells. J Cell Sci. 1999;112:1685-96. (FC)

9.Koritschoner NP, Madruga J, Knespel S, Blendinger G, Anzinger B, Otto A, et al. The nuclear orphan receptor TR4 promotes proliferation of myeloid progenitor cells. Cell Growth Differ. 2001;12:563-72. (FC)

10.Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)

11.Juul-Madsen HR, Dalgaard TS, Røntved CM, Jensen KH, Bumstead N. Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes. Poult Sci. 2006;85:986-98. (FC)

12.Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)

13.Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)

14.Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek’s disease virus. Viral Immunol. 2009;22:321-7. (FC)

15.Sunkara LT, Achanta M, Schreiber NB, Bommineni YR, Dai G, Jiang W, et al. Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression. PLoS One. 2011;6(11):e27225. (FC)

16.Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)

17.Chrząstek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken. Pol J Vet Sci. 2013;16:551-4. (FC)

18.Fernandes Filho T, Fávaro C Jr, Ingberman M, Beirão BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)

19.Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)

20.Olias P, Meyer A, Klopfleisch R, Lierz M, Kaspers B, Gruber AD. Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi. Vet Res. 2013;44:10. (IHC-FS, Pigeon Reactivity)