Goat Anti-Mouse IgG(H+L), Rat ads-HRP抗體 | 貨號1034-05

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原廠連結：	https://www.southernbiotech.com
相關下載：	Goat Anti-Mouse IgG(H+L), Rat ads-HRP抗體操作手冊

特點

Goat Anti-Mouse IgG(H+L), Rat ads-HRP抗體 | 貨號1034-05 規格1.0 mL

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Rat ads-TRITC (SB Cat. No. 1034-03) and DAPI.

產品介紹: Goat Anti-Mouse IgG(H+L), Rat ads-HRP抗體，具HRP標誌。針對mouse IgG₁, IgG_2a, IgG_2b, IgG_2c, and IgG₃ 輕鏈及重鏈以及mouse IgM and IgA輕鏈具特異性。來源為Pooled antisera from goats hyperimmunized with mouse IgG。Isotype為Goat IgG。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

Specificity	Reacts with the heavy and light chains of mouse IgG₁, IgG_2a, IgG_2b, IgG_2c, and IgG₃ and with the light chains of mouse IgM and IgA
Source	Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption	Rat immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification	Affinity chromatography on mouse IgG covalently linked to agarose
Isotype	Goat IgG
Format/Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA ^1-5 FLISA Flow Cytometry ^6-9 Other referenced applications for relevant formats include – ELISpot ¹⁰ Western Blot ^11-17 Immunohistochemistry-Frozen Sections ¹⁸ Immunocytochemistry ¹⁹
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794340

References

引用文獻

Shichkin VP, Spivak NY. Cytokine-deficient mice as a model for generation of autologous anti-cytokine monoclonal antibodies. Immunol Lett. 2006;102:148-57. (ELISA)
van Olffen RW. Modulating T cell homeostasis via TNF and TNFR superfamily members: characterization and function of effector & regulatory T cells [dissertation]. Amsterdam (Netherlands): University of Amsterdam, 2009. (ELISA)
Shichkin VP. Immune response of IL-4-knockout mice to low-dose immunization with autologous IL-4. WebmedCentral IMMUNOLOGY. 2011;2(12):WMC002826. (ELISA)
Suzuki K, Tsunoda H, Omiya R, Matoba K, Baba T, Suzuki S, et al. Structure of the plexin ectodomain bound by semaphorin-mimicking antibodies. PLoS One. 2016;11(6):e0156719. (ELISA)
Benyacoub J, Blum-Sperisen S, Rochat F, von der Weid T, inventors; Nestec S.A., assignee. Probiotics for use in expecting female mammals for enhancing the immunity of their offspring. United States patent application publication US 2017/0006908 A1. 2017 Jan 12. (ELISA)
Corral L, Takizawa H, Hanke T, Jamieson AM, Raulet DH. A new monoclonal antibody reactive with several Ly49 NK cell receptors mediates redirected lysis of target cells. Hybridoma. 1999;18:359-66. (FC)
Krasinskas AM, Eiref SD, McLean AD, Kreisel D, Gelman AE, Popma SH, et al. Replacement of graft-resident donor-type antigen presenting cells alters the tempo and pathogenesis of murine cardiac allograft rejection. Transplantation. 2000;70:514-21. (FC)
Kreisel D, Petrowsky H, Krasinskas AM, Krupnick AS, Szeto WY, McLean AD, et al. The role of passenger leukocyte genotype in rejection and acceptance of rat liver allografts. Transplantation. 2002;73:1501-7. (FC)
Arefanian H, Tredget EB, Rajotte RV, Korbutt GS, Gill RG, Rayat GR. Combination of anti-CD4 with anti-LFA-1 and anti-CD154 monoclonal antibodies promotes long-term survival and function of neonatal porcine islet xenografts in spontaneously diabetic NOD mice. Cell Transplant. 2007;16:787-98. (FC)
Huang C, Hatzi K, Melnick A. Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat Immunol. 2013;14:380-8. (ELISPOT)
Gill MB, Perez-Polo JR. Bax shuttling after rotenone treatment of neuronal primary cultures: effects on cell death phenotypes. J Neurosci Res. 2009;87:2047-65. (WB)
Perez-Polo JR, Reilly CB, Rea HC. Oxygen resuscitation after hypoxia ischemia stimulates prostaglandin pathway in rat cortex. Int J Dev Neurosci. 2011;29:639-44. (WB)
Durham-Lee JC, Wu Y, Mokkapati VU, Paulucci-Holthauzen AA, Nesic O. Induction of angiopoietin-2 after spinal cord injury. Neuroscience. 2012;202:454-64. (WB)
Bronner DN, O’Riordan MX, He Y. Caspase-2 mediates a Brucella abortus RB51-induced hybrid cell death having features of apoptosis and pyroptosis. Front Cell Infect Microbiol. 2013;3:83. (WB)
Guptarak J, Wanchoo S, Durham-Lee J, Wu Y, Zivadinovic D, Paulucci-Holthauzen A, et al. Inhibition of IL-6 signaling: A novel therapeutic approach to treating spinal cord injury pain. Pain. 2013;154:1115-28. (WB)
Xu X, Araki K, Li S, Han J, Ye L, Tan WG, et al. Autophagy is essential for effector CD8+ T cell survival and memory formation. Nat Immunol. 2014;15:1152-61. (WB)
Staffler G, Landlinger C, Le Bras M, inventors; AFFiRiS AG, assignee. Vaccines and monoclonal antibodies targeting truncated variants of osteopontin and uses thereof. United States patent application publication US 2017/0137509 A1. 2017 May 18. (WB)
Kawasaki T, Nishio T, Kawaguchi S, Kurosawa H. Spatiotemporal distribution of GAP-43 in the developing rat spinal cord: a histological and quantitative immunofluorescence study. Neurosci Res. 2001;39:347-58. (IHC-FS)
Zeineldin R, Rosenberg M, Ortega D, Buhr C, Chavez MG, Stack MS, et al. Mesenchymal transformation in epithelial ovarian tumor cells expressing epidermal growth factor receptor variant III. Mol Carcinog. 2006;45:851-60. (ICC)

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