Rabbit Anti-Mouse IgG(H+L)-HRP抗體 | 貨號6170-05

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原廠連結：	https://www.southernbiotech.com
相關下載：	Rabbit Anti-Mouse IgG(H+L)-HRP抗體操作手冊

特點

Rabbit Anti-Mouse IgG(H+L)-HRP抗體 | 貨號6170-05

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

Proteins derived from outer membrane vesicles of patients with kidney stones were resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-EF-Tu followed by Rabbit Anti-Mouse IgG(H+L)-HRP (SB Cat. No. 6170-05) and chemiluminescent detection. Image from Amimanan P, Tavichakorntrakool R, Fong-ngern K, Sribenjalux P, Lulitanond A, Prasongwatana V, et al. Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation. Sci Rep. 2017;7:2953. Figure 4

產品介紹: Rabbit Anti-Mouse IgG(H+L)-HRP抗體，具HRP標誌。針對mouse IgG輕鏈及重鏈以及mouse IgM and IgA輕鏈具特異性。來源為Pooled antisera from rabbits hyperimmunized with mouse IgG。Isotype為Rabbit IgG。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

Specificity	Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source	Pooled antisera from rabbits hyperimmunized with mouse IgG
Cross Adsorption	None; may react with immunoglobulins from other species
Purification	Affinity chromatography on mouse IgG covalently linked to agarose
Isotype	Rabbit IgG
Format/Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA ^1-4 FLISA Other referenced applications for relevant formats include – ELISpot ⁵ Immunohistochemistry-Frozen Sections ^6,7 Immunoprecipitation ⁸ Western Blot ^9-11
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796243

引用文獻

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Cat. No.	Product Description	Clone	Size
6170-30	Rabbit Anti-Mouse IgG(H+L)-Alexa Fluor^® 488	Polyclonal	1.0 mg
6170-31	Rabbit Anti-Mouse IgG(H+L)-Alexa Fluor^® 647	Polyclonal	1.0 mg
6170-04	Rabbit Anti-Mouse IgG(H+L)-AP	Polyclonal	1.0 mL
6170-08	Rabbit Anti-Mouse IgG(H+L)-BIOT	Polyclonal	1.0 mg
6170-02	Rabbit Anti-Mouse IgG(H+L)-FITC	Polyclonal	1.0 mg
6170-05	Rabbit Anti-Mouse IgG(H+L)-HRP	Polyclonal	1.0 mL
6170-01	Rabbit Anti-Mouse IgG(H+L)-UNLB	Polyclonal	1.0 mg

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