Mouse Anti-Human IgE Fc-FITC抗體 | 貨號9250-02
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Mouse Anti-Human IgE Fc-FITC抗體 | 貨號9250-02 規格1.0 ml (0.5 mg/mL)
美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。
二級抗體 (Secondary antibodies) 應用:
二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。
產品介紹: Mouse Anti-Human IgE Fc-FITC抗體，具FITC標誌。免疫源為PS human IgE myeloma protein針對Human IgE Fc具特異性。Isotype為Mouse IgG2aκ。Isotype Control Mouse IgG2a-FITC。 緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。
| Clone | HP6029 |
| Isotype | Mouse IgG2aκ |
| Isotype Control | Mouse IgG2a-FITC |
| Immunogen | PS human IgE myeloma protein |
| Specificity | Human IgE Fc |
| Format/Conjugate | FITC (Fluorescein) |
| Buffer Formulation | Phosphate buffered saline containing 0.1% sodium azide |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Storage & Handling | 2-8°C; Avoid exposure to light Please refer to product specific SDS |
| Applications |
Applications for relevant formats of this clone include – ELISA – Quality tested 1-8,11 |
| Recommended Dilutions | Please refer to product specific Technical Bulletin |
| RRID | AB_2796717 |
SBA熱銷商品 7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 | DAPI螢光封片劑 | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I | 一級抗體
| Cat. No. | Product Description | Clone | Size |
| 9250-04 | Mouse Anti-Human IgE Fc-AP | HP6029 | 1.0 mL |
| 9250-08 | Mouse Anti-Human IgE Fc-BIOT | HP6029 | 0.5 mg |
| 9250-02 | Mouse Anti-Human IgE Fc-FITC | HP6029 | 0.5 mg |
| 9250-05 | Mouse Anti-Human IgE Fc-HRP | HP6029 | 1.0 mL |
| 9250-01 | Mouse Anti-Human IgE Fc-UNLB | HP6029 | 0.5 mg |
引用文獻
References
- Agea E, Russano A, Bistoni O, Mannucci R, Nicoletti I, Corazzi L, et al. Human CD1-restricted T cell recognition of lipids from pollens. J Exp Med. 2005;202:295-308. (ELISA)
- Milovanovic M, Heine G, Zuberbier T, Worm M. Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production. Clin Exp Allergy. 2009;39:671-8. (ELISA)
- Heine G, Dahten A, Hilt K, Ernst D, Milovanovic M, Hartmann B, et al. Liver X receptors control IgE expression in B cells. J Immunol. 2009;182:5276-82. (ELISA)
- Milovanovic M, Drozdenko G, Weise C, Babina M, Worm M. Interleukin-17A promotes IgE production in human B cells. J Invest Dermatol. 2010;130:2621-8. (ELISA)
- Weise C, Hilt K, Milovanovic M, Ernst D, Rühl R, Worm M. Inhibition of IgE production by docosahexaenoic acid is mediated by direct interference with STAT6 and NFκB pathway in human B cells. J Nutr Biochem. 2011;22:269-75. (ELISA, ELISPOT)
- Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
- Qian Y, Jeong JS, Abdeladhim M, Valenzuela JG, Aoki V, Hans-Filhio G, et al. IgE anti-LJM11 sand fly salivary antigen may herald the onset of Fogo selvagem in endemic Brazilian regions. J Invest Dermatol. 2015;135:913-5. (ELISA)
- Bundhoo A, Paveglio S, Rafti E, Dhongade A, Blumberg RS, Matson AP. Evidence that FcRn mediates the transplacental passage of maternal IgE in the form of IgG anti-IgE/IgE immune complexes. Clin Exp Allergy. 2015 Feb 5. doi: 10.1111/cea.12508. [Epub ahead of print]. (ELISA)
- Martos G, Pineda-Vadillo C, Miralles B, Alonso-Lebrero E, López-Fandiño R, Molina E, et al. Identification of an IgE reactive peptide in hen egg riboflavin binding protein subjected to simulated gastrointestinal digestion. J Agric Food Chem. 2012;60:5215-20. (WB)
- Benedé S, López-Expósito I, Giménez G, Grishina G, Bardina L, Sampson HA, et al. In vitro digestibility of bovine β-casein with simulated and human oral and gastrointestinal fluids. Identification and IgE-reactivity of the resultant peptides. Food Chem. 2014;143:514-21. (WB)
- Kleine-Tebbe J, Hamilton RG, Roebber M, Lichtenstein LM, MacDonald SM. Purification of immunoglobulin E (IgE) antibodies from sera with high IgE titers. J Immunol Methods. 1995;179:153-64. (Purification, ELISA)