LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)慢病毒表現載體 | 貨號LV010162

特點
LUCIFERASE (FIREFLY) Lentiviral Vector (CMV)慢病毒表現載體 | 貨號LV010162
產品描述: pLenti-III-mKate2-2A-luc (CMV promoter) (Gene Insert Luciferase (Firefly) 架構於pLenti-III-CMV表現載體上,具有Luciferase基因,構成pLenti-III-mKate2-2A-luc (CMV promoter)。慢病毒載體,可結合packaging mix (改良式 病毒包裝系統 LV003) 以在多種宿主細胞或動物模型中過表達。 該構建體含有用於監測轉染效率的Luciferase基因,可以Monitor轉染效率。可直接以非病毒質粒轉染 (non-viral plasmid transfection) 立即表達,或經由包裝成慢病毒顆粒。

| Description | mKate2 linked to a firefly Luciferase reporter by a 2A peptide, expression driven by CMV promoter. |
| Product Application |
· Direct non-viral plasmid transfection for immediate expression · Package into Lentiviral particles for high efficiency and stably integrated expression |
| Gene Insert | Luciferase (Firefly) |
| Vector | pLenti-III-CMV |
| Vector Size | 10506 bp |
| Insert Size | 0 bp |
| Reporter/Tag | No Tags |
| Promoter | CMV |
| Selection Marker | Bacterial: Kanamycin; Mammalian: Puromycin |
| Format | Plasmid |
| Viral Packaging | abm recommends using Cat# G074, Lentifectin™ in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat# LV003, 2nd Generation Packaging Mix. Alternatively, you can also use Cat# LV053, 3rd Generation Packaging Mix |
| Appearance | Clear Liquid |
| Storage Buffer | 10mM Tris-HCI, 1mM EDTA, pH8.0 |
| Stability | 1 year when stored at -20°C or lower in a non-frost free freezer |
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