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Goat Anti-Rabbit IgM-HRP抗體 | SBA貨號4020-05

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原廠連結:
https://www.southernbiotech.com
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Goat Anti-Rabbit IgM-HRP抗體操作手冊

特點

Goat Anti-Rabbit IgM-HRP抗體 | 貨號4020-05  規格1.0 ml

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體,目前除了一般常見的多株與單株二級抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白(immunoglobulin , Ig)分子的多克隆(polyclonal) 和單克隆抗體 (monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用,並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用,例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記,這使得能夠通過比色,化學發光和螢光技術進行檢測。例如,酶辣根過氧化物酶(enzymes horseradish peroxidase , HRP)和鹼性磷酸酶(alkaline phosphatase , AP)通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素(fluorescein , FITC),藻紅蛋白(phycoerythrin , PE)和花青(cyanine , CY)的螢光染料在螢光的應用中是必不可少的,包括流式細胞術,免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大,可增加的靈敏度。

產品介紹:  Goat Anti-Rabbit IgM-HRP抗體,具HRP標誌。針對rabbit IgM重鏈(heavy chain)具特異性。來源為Pooled antisera from goats hyperimmunized with rabbit IgM。Isotype為Goat IgG。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

Specificity Reacts with the heavy chain of rabbit IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgM
Cross Adsorption Rabbit IgG; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled rabbit IgM covalently linked to agarose
Isotype Goat IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include –
ELISA 1-5
FLISA
Other referenced applications for relevant formats include –
Flow Cytometry 6-13
ELISPOT 3,13
Immunohistochemistry-Frozen Sections 14,15
Immunohistochemistry-Paraffin Sections 16
Western Blot 17-20
Separation 10,21
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795928

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

References

  1. Holdsworth RJ, McKenzie H, Parratt D, Irving AD, Cuschieri A. The role of the spleen in the immune response following naturally acquired exposure to encapsulated bacteria. Int J Exp Pathol. 1990;71:835-43. (ELISA)
  2. Taylor-Robinson AW, Phillips RS. B cells are required for the switch from Th1- to Th2-regulated immune responses to Plasmodium chabaudi chabaudi infection. Infect Immun. 1994;62:2490-98. (ELISA)
  3. Yamashita T, Freigang S, Eberle C, Pattison J, Gupta S, Napoli C, et al. Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring. Circ Res. 2006;99:e51-64. (ELISA, ELISPOT)
  4. Quan JH, Hassan HA, Cha G, Shin D, Lee Y. Antigenemia and specific IgM and IgG antibody responses in rabbits infected with Toxoplasma gondii. Korean J Parasitol. 2009;47:409-12. (ELISA)
  5. Isik G, Sliepen K, van Montfort T, Sanders RW. Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL. PLoS One. 2014;9(9):e107683. (ELISA)
  6. Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: “positive” selection in appendix of normal and VH-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)
  7. Sehgal D, Mage RG, Schiaffella E. VH mutant rabbits lacking the VH1a2 gene develop a2+ B cells in the appendix by gene conversion-like alteration of a rearranged VH4 gene. J Immunol. 1998;160:1246-55. (FC)
  8. Yang G, Obiakor H, Sinha RK, Newman BA, Hood BL, Conrads TP, et al. Activation-induced deaminase cloning, localization, and protein extraction from young VH-mutant rabbit appendix. Proc Natl Acad Sci USA. 2005;102:17083-8. (FC)
  9. Pospisil R, Kabat J, Mage RG. Characterization of rabbit CD5 isoforms. Mol Immunol. 2009;46:2456-64. (FC)
  10. Yang J, Pospisil R, Mage RG. Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system. Dev Comp Immunol. 2009;33:697-708. (FC, Sep)
  11. Yang J, Pospisil R, Ray S, Milton J, Mage RG. Investigations of a rabbit (Oryctolagus cuniculus) model of systemic lupus erythematosus (SLE), BAFF and its receptors. PLoS One. 2009;4(12):e8494. (FC)
  12. Mage RG, Rai G. A rabbit model of systemic lupus erythematosus, useful for studies of neuropsychiatric SLE. Systemic Lupus Erythematosus, Almoallim H ed. 2012. (FC)
  13. Baranyi M, Cervenak J, Bender B, Kacskovics I. Transgenic rabbits that overexpress the neonatal Fc receptor (FcRn) generate higher quantities and improved qualities of anti-thymocyte globulin (ATG). PLoS One. 2013;8(10):e76839. (FC, ELISPOT)
  14. Schiaffella E, Sehgal D, Anderson AO, Mage RG. Gene conversion and hypermutation during diversification of VH sequences in developing splenic germinal centers of immunized rabbits. J Immunol. 1999;162:3984-95. (IHC-FS)
  15. Sehgal D, Obiakor H, Mage RG. Distinct clonal Ig diversification patterns in young appendix compared to antigen-specific splenic clones. J Immunol. 2002;168:5424-33. (IHC-FS)
  16. Rosner A, Moiseeva E, Rabinowitz C, Rinkevich B. Germ lineage properties in the urochordate Botryllus schlosseri – From markers to temporal niches. Dev Biol. 2013;384:356-74. (IHC-PS)
  17. Gibson JR, Chart H, Owen RJ. Intra-strain variation in expression of lipopolysaccharide by Helicobacter pylori. Lett Appl Microbiol. 1998;26:399-403. (WB)
  18. Chart H, Cheasty T, Willshaw GA. Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli. J Med Microbiol. 2002;51:1050-4. (WB)
  19. Chart H, Cheasty T, de Pinna E, Siovanes L, Wain J, Alam D, et al. Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections. J Med Microbiol. 2007;56:1161-6. (WB)
  20. Furuya K, Omura M, Kudo S, Sugiura W, Azuma H. Recognition profiles of microsporidian Encephalitozoon cuniculi polar tube protein 1 with human immunoglobulin M antibodies. Parasite Immunol. 2008;30:13-21. (WB)
  21. Pospisil R, Fitts MG, Mage RG. CD5 is a potential selecting ligand for B cell surface immunoglobulin framework region sequences. J Exp Med. 1996;184:1279-84. (Sep)

 

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