TGF-β1酵素免疫分析套組 | 貨號K4342-100

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特點
TGF-beta1 (human) ELISA Kit產品描述:TGF-β1是參與細胞生長,分化和炎症途徑的多功能細胞因子。 BioVision的人TGF-β1酶聯免疫試劑盒是基於三明治酶聯免疫吸附試驗的標準原理。將特異於人TGF-β1的小鼠單克隆抗體包被在96孔板上。將標準品和測試樣品加入到孔中,並且樣品中存在的TGF-β1被固定的抗體結合。隨後添加來自特異於TGF-β1的山羊的生物素化多克隆抗體。用PBS或TBS緩衝液洗去未結合的生物素化抗體後,將抗生物素蛋白 – 生物素 – 過氧化物酶複合物加入孔中。再次用PBS或TBS緩衝液洗滌孔以除去未結合的綴合物。使用HRP底物TM B顯現HRP酶促反應。 B被HRP催化產生藍色產物,加入酸性終止溶液後變成黃色。黃色的密度與捕獲在平板上的人TGF-β1成比例。該ELISA試劑盒與TGF-β2,TGF-β3和TGF-β5顯示出<1%的交叉反應性。檢測範圍:15.6 pg / ml – 1000 pg / ml。靈敏度:<1 pg / ml。

| Cat # +Size | K4342-100 |
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | human |
| Applications | Quantitative protein detection, establishing normal range. |
| Features & Benefits | • Easy, convenient and reliable method to assay for human TGF-beta 1. • Detection Range: 15.6-1000 pg/ml. Sensitivity: < 1 pg/ml. • This ELISA kit shows <1% cross-reactivity with TGF?2, TGF?3 & TGF?5. • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. |
| Kit Components | • 96 wells coated with anti-human TGF-β1 antibody, 1 Microplate • Lyophilized recombinant human TGF-β1 (25KDa) standard (10 ng/vial) • Biotinylated anti-human TGF-β1 antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution |
