困難梭菌二來毒素B亞單位多株抗體 | Binary Toxin, B subunit貨號 ACdBTSB

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特點
產品介紹: Binary Toxin, B subunit困難梭菌二來毒素B亞單位多株抗體, 其免疫原自二來毒素B亞單位進行雞隻免疫。在弗氏佐劑中進行多次免疫後,收集蛋,並將IgY純化。在ELISA中測試了該抗體 – 使用1μg二元B毒素的塗層,劑量反應曲線的中點為110ng / ml。 該抗體可以用作為ELISA格式的ACdBTSB-B或ACdBTSB-HRP作為捕獲抗體(1ug /孔)。對於特定的測定條件,應該確定最佳的工作稀釋度。

使用雞抗體的好處:
- 雞非哺乳類動物,和哺乳類抗原有高親和性,且避免和哺乳類抗原產生cross-reaction。
- 為最人道的多株抗體產製方法。只需從雞蛋萃取蛋白,取樣方便,不傷害動物。
- 每隻雞可以產生的抗體量約3 g IgY/月,是兔子的10-20倍多。且產製速度比起兔子快上許多,最快25天即可取得高力價(titer)抗體。
- 雞IgY的Fc region和哺乳類IgG的不同:
- 可降低對哺乳動物的背景值。
- 不會活化哺乳類補補體系統。
- 不會與哺乳類Fc recentors結合。
- 無法和常規的protein A(Staphylococcus aureus)、protein G(Streptococcus sp.)、或protein L(Peptostreptococcus magnus)等免疫球蛋白-蛋白結合反應。

- 由於IgY取得方便,雞蛋可以儲存較長的時間,並可純化取得較佳的titer。
- 相對於兔子,雞的開銷較便宜。
- IgY有以下數種和哺乳類IgG類似的特徵:
- 為二價體。
- 可由papain分解成divalent Fab fragment。
- 適用酵素、生物素(biotin)、金粒子等標定物。
| Full Name | Affinity Purified Chicken anti-Clostridium difficile Binary Toxin, B Subunit |
| Host | Chicken |
| Quantity | 0.1 mg |
| Volume | 0.05 mL |
| Type | Affinity purified |
| Physical State | Liquid |
| Concentration | 2.0 mg/mL |
| Diluent | Phosphate buffered saline‚ pH 7.2 |
| Preservative | 0.075% sodium azide |
| Procedure | Chickens were immunized with highly purified Binary Toxin, B subunit, purified from Clostridium difficile. After multiple immunizations in Freund’s adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Binary Toxin, Subunit B was immunoaffinity purified using the immunogen immobilized on a solid support. |
| Specifications | This antibody has been tested in ELISA — the midpoint of the dose response curve is 110 ng/ml using a coating of 1 ug of Binary B toxin. This antibody can be used as a capture antibody (1 ug/well) with ACdBTSB-B or ACdBTSB-HRP in an ELISA format.Optimal working dilutions should be determined for your particular assay conditions. |
雞IgY抗體純化試劑盒 | Anti-Chicken多株抗體-HRP | 抗鼠瘧原蟲病毒-68 ORF59 | 炭疽芽孢桿菌致死因子A-FITC | anti-炭疽芽孢桿菌致死因子A | 抗犬心臟蟲 | 抗肉毒桿菌神經毒素E型輕鏈 | 抗困難梭狀桿菌谷氨酸脫氫酶(GDH)
| 貨號 | IgY抗體商品 | (Affinity purified) |
| DAIgYFab | Donkey Anti-Chicken IgY Fab | 0.5 mg |
| DAIgYFab-B |
Donkey Biotinylated Anti-Chicken IgY Fab
|
0.5 mg (BIOTIN) |
| DAIgYFab-F | Donkey FITC-labeled Anti-Chicken IgY Fab | 0.5 mg (FITC) |
| DAIgYFab-HRP | Donkey HRP-labeled Anti-Chicken IgY Fab | 0.5 mg (HRP) |
| AIgYFab | Goat Anti-Chicken IgY Fab | 0.5 mg |
Normal Chicken IgY | Normal Duck IgY| Normal Goose IgY| Anti-IgY | Anti IgY Immunoprecipitation (IP) Reagents | Other Anti-Chicken Immunoglobulins
