抗犬心臟蟲多株抗體 | heartworms (Dirofilaria immitus) Antibody貨號ACH

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特點
產品介紹: 抗犬心臟蟲多株抗體heartworms (Dirofilaria immitus) Antibody, 其免疫原自雄性和雌性心絲蟲(Dirofilaria immitus)的提取物進行雞隻免疫。 以Freund’s adjuvant弗氏佐劑中進行多次免疫後,收集蛋液,並將IgY純化。抗體是從固相免疫吸附劑中免疫親和純化。該抗體適用於ELISA,Western Blotting和側向流動應用。 Western印蹟的推薦稀釋度在1:1,000和1:10,000之間,ELISA捕獲在1:200到1:1000之間。
使用雞抗體的好處:
- 雞非哺乳類動物,和哺乳類抗原有高親和性,且避免和哺乳類抗原產生cross-reaction。
- 為最人道的多株抗體產製方法。只需從雞蛋萃取蛋白,取樣方便,不傷害動物。
- 每隻雞可以產生的抗體量約3 g IgY/月,是兔子的10-20倍多。且產製速度比起兔子快上許多,最快25天即可取得高力價(titer)抗體。
- 雞IgY的Fc region和哺乳類IgG的不同:
- 可降低對哺乳動物的背景值。
- 不會活化哺乳類補補體系統。
- 不會與哺乳類Fc recentors結合。
- 無法和常規的protein A(Staphylococcus aureus)、protein G(Streptococcus sp.)、或protein L(Peptostreptococcus magnus)等免疫球蛋白-蛋白結合反應。

- 由於IgY取得方便,雞蛋可以儲存較長的時間,並可純化取得較佳的titer。
- 相對於兔子,雞的開銷較便宜。
- IgY有以下數種和哺乳類IgG類似的特徵:
- 為二價體。
- 可由papain分解成divalent Fab fragment。
- 適用酵素、生物素(biotin)、金粒子等標定物。
| Full Name | Affinity-purified Chicken-anti-Canine Heartworm |
| Host | Chicken |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Type | Affinity purified |
| Physical State | Liquid |
| Concentration | 1.0 mg/mL |
| Diluent | Phosphate buffered saline‚ pH 7.2 |
| Preservative | 0.1% Sodium Azide |
| Procedure | Chickens were immunized with extracts from both male and female heartworms (Dirofilaria immitus). After multiple immunizations in Freund’s adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant. |
| Specifications | This anti-canine heartworm is suitable for ELISA, Western Blotting and lateral flow applications. Recommended dilutions for Western Blots is between 1:1,000 and 1:10,000 and for ELISA capture is between 1:200 and 1:1000. |
雞IgY抗體純化試劑盒 | Anti-Chicken多株抗體-HRP | 抗鼠瘧原蟲病毒-68 ORF59 | 炭疽芽孢桿菌致死因子A-FITC | anti-炭疽芽孢桿菌致死因子A | 抗犬心臟蟲 | 抗肉毒桿菌神經毒素E型輕鏈 | 抗困難梭狀桿菌谷氨酸脫氫酶(GDH)
| 貨號 | IgY抗體商品 | (Affinity purified) |
| DAIgYFab | Donkey Anti-Chicken IgY Fab |
0.5 mg
|
| DAIgYFab-B |
Donkey Biotinylated Anti-Chicken IgY Fab
|
0.5 mg (BIOTIN) |
| DAIgYFab-F | Donkey FITC-labeled Anti-Chicken IgY Fab | 0.5 mg (FITC) |
| DAIgYFab-HRP | Donkey HRP-labeled Anti-Chicken IgY Fab | 0.5 mg (HRP) |
| AIgYFab | Goat Anti-Chicken IgY Fab | 0.5 mg |
Normal Chicken IgY | Normal Duck IgY| Normal Goose IgY| Anti-IgY | Anti IgY Immunoprecipitation (IP) Reagents | Other Anti-Chicken Immunoglobulins
