固定化過氧化氫酶珠| Immobilized Catalase Beads 貨號7931

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原廠連結：	http://www.biovision.com
相關下載：	Immobilized Catalase Beads操作手冊

特點

蛋白質純化樹脂系列商品

重組蛋白的表達和純化對於研究protein regulation蛋白質調節，Structure結構及function功能研究為重要研究工具。大多數重組蛋白質表達為具有短親和力的融合蛋白標籤或小蛋白質，如GST。 GST為特異性結合還原型穀胱甘肽。 BioVision的GlutathioneSepharose®通過共價偶聯glutathione穀胱甘肽、與epoxy-activated環氧活化的4％cross-linked sepharose beads交聯瓊脂糖凝膠，來製備形成穩定的硫醚鍵thioether linkage。Heparin-Sepharose®廣泛用於純化血清蛋白包括coagulation factors凝血因子、lipases脂肪酶、lipoproteins脂蛋白，hormone receptors激素受體和growth factors生長因子。 BioVision公司的Heparin-Sepharose®通過共價連接肝素與環氧自由基6％交聯而製備瓊脂糖珠。優化了穀胱甘肽和肝素 – 瓊脂糖凝膠的偶聯技術高結合能力（≥5 mg GST / ml Glutathione-Sepharose resin ; Heparin-Sepharose® resin≥0.4mg肝素結合蛋白/ ml），具高流速和最小浸出配體。

Immobilized Catalase Beads | 貨號7931

An ideal solution to limit the hydrogen peroxide interferance from sample analytes in downstream analyses.

產品介紹: BioVision的固定化過氧化氫酶珠是用於HTP製備含有過氧化氫或不需要的分析物的樣品的理想溶液，其產生干擾下游分析的過氧化氫。通過將過氧化氫酶與6％交聯的瓊脂糖珠的專有共價偶聯製備固定的過氧化氫酶珠。優化了偶聯技術以獲得高產率和回收過氧化氫酶活性以及確保固定化酶的長期穩定性。

Highlights

Applications:
• The immobilized catalase beads can be used to eliminate or reduce sample background generated from hydrogen peroxide in assays that also use hydrogen peroxide for signal detection. Centrifugation to remove the immobilized catalase beads will prevent interference of catalase in assays that use hydrogen peroxide/HRP based mechanisms for detection.
• If unwanted interfering substances are removed during sample prep by oxidation, the reaction can be carried out to completion with simultaneous removal of hydrogen peroxide
User Supplied Reagents and Equipments:
• 96-well polypropylene plate
• Centrifuge for 96-well plates Protocol
1. Mix beads to generate homogeneous 50% suspension.
2. Add 10 µl of the 50 % slurry of the immobilized catalase beads to 50 µl sample of interest.
3. Incubate the sample at room temperature for fifteen minutes.
4. Spin down the sample at 1000 x g for a minute at RT to remove the beads.
5. Collect the supernatant and use for downstream analyses

蛋白質定量商品 Coomassie blue蛋白質定量分析套組 | BCA蛋白測定試劑盒(低濃度10-600ug/ml)| 5％耐洗滌 | BCA蛋白測定試劑盒 (還原劑兼容)

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