快速純化His-tag表現蛋白(含Buffer) | 貨號K6567

Kit with Hi-Bind™ Ni QR Agarose Bead columns, & loading, washing & elution buffers for small scale purification of poly-histidine tagged proteins

產品介紹: BioVision的Ni-IDA Spin Columns是快速小量純化poly-histidine-tagged proteins多組His-tag的蛋白質之理想選擇。每一個columns預填充100μL高性能Hi-Bind™Ni QR瓊脂糖珠（Cat。＃6562），可以高效力捕獲和純化Histidine-tag表現蛋白。每管小量純化可高達2.8毫克的Histidine-tag的蛋白質。柱內的最佳化小體積之珠子, 在正常表達水平下，允許通過更強烈結合Histidine-tag表現蛋白，有效置換非標記蛋白質雜質，並提高最終產物的純度。純化的產率和純度是蛋白質特異性，並且取決於各種因素，如蛋白表達水平，結構，溶解度等。填充在每個柱中的珠子比Ni-NTA吸附劑型式提供高達25％的容量。K6567試劑盒還包括column loading柱裝載，wash洗滌液和elution buffers洗脫緩衝液，快速純化Histidine-tag的蛋白質。

Cat # +Size	K6567-25
Size	25 columns
Highlights	Salient Features: Binding Capacity: Upto 2.8 mg poly-histidine tagged proteins per column. Column Content: 25 Ni-IDA spin columns, each containing 100 µl high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562). Bead content: A 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol. Applications: • Ideal for small scale, one – step purification of poly-histidine tagged recombinant proteins under native conditions. • Ideal for small scale, one – step purification of native proteins and peptides that have an affinity for metal ions. • Screening of expression levels in a HTP manner. • Applicable for purification under native and denaturing conditions.