肝素瓊脂糖 | Heparin Sepharose貨號6553
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蛋白質純化樹脂系列商品
重組蛋白的表達和純化對於研究protein regulation蛋白質調節，Structure結構及function功能研究為重要研究工具。大多數重組蛋白質表達為具有短親和力的融合蛋白標籤或小蛋白質，如GST。 GST為特異性結合還原型穀胱甘肽。 BioVision的GlutathioneSepharose®通過共價偶聯glutathione穀胱甘肽、與epoxy-activated環氧活化的4％cross-linked sepharose beads交聯瓊脂糖凝膠，來製備形成穩定的硫醚鍵thioether linkage。Heparin-Sepharose®廣泛用於純化血清蛋白包括coagulation factors凝血因子、lipases脂肪酶、lipoproteins脂蛋白，hormone receptors激素受體和growth factors生長因子。 BioVision公司的Heparin-Sepharose®通過共價連接肝素與環氧自由基6％交聯而製備瓊脂糖珠。優化了穀胱甘肽和肝素 – 瓊脂糖凝膠的偶聯技術高結合能力（≥5 mg GST / ml Glutathione-Sepharose resin ; Heparin-Sepharose® resin≥0.4mg肝素結合蛋白/ ml），具高流速和最小浸出配體。
Heparin Sepharose 肝素瓊脂糖 | 貨號6553
High binding capacity beads for affinity purification of heparin binding proteins
產品介紹: 肝素瓊脂糖通過共價連接肝素與環氧活化的6％交聯瓊脂糖凝膠珠來製備。 優化偶聯以產生高結合能力，並且每ml凝膠可大於0.4mg肝素結合蛋白（例如凝血酶）。
相關商品 Ni-IDA Spin Columns (His-tag) | Ni-IDA 快速純化His-tag表現蛋白 (含Buffer) | Jacalin-瓊脂糖 (IgA) | Calmodulin-Sepharose Beads(鈣調蛋白) | Albumin-IgG 去除Beads | Cibacron Blue-Agarose Beads | 肝素瓊脂糖
| Highlights | • CONTENTS: Supplied as a 50% slurry in 20% ethanol/H₂O; >2.5 mg heparin per ml Sepharose beads. • FEATURES: Heparin-beads have been widely used in affinity purification of various heparin-binding proteins or ligand, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and other ligands. BioVison’s Heparin-Sepharose is designed for the purpose of purification of these kinds of proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This Heparin-Sepharose formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10. • APPLICATIONS: Purification of heparin-binding proteins, enzymes or other ligands. |
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