ExKine™ Pro 動物培養細胞/組織總蛋白提取試劑盒貨號KTP3007

ExKine™ Pro Total Protein Extraction Kit (Protein yield: 2-8 mg/mL)

ExKine™ Pro 動物培養細胞/組織總蛋白提取試劑盒(ExKine™ Pro Total Protein Extraction Kit), 是新一代超快速蛋白提取工具; 更多和更多證據表明，最常用的RIPA緩衝液(RIPA buffer)可能會導致蛋白質隨機丟失，導致許多困難的數據這很難解釋。 該試劑盒採用離心柱提取技術(centrifugal column extraction technologyc)結合優化的裂解緩衝液(optimized lysis buffer)進行提取更快速有效地檢測總蛋白(total protein)，使 WB 結果更準確。 提取系統可低至 20 μL採用離心柱提取蛋白質，有效解決樣品量少的問題。 該套件適用於SDS-PAGE、WB 和 IP 等應用。可用於裂解哺乳動物(mammalian adherent cells)貼壁細胞、非貼壁細胞和組織的快速高效解決方案組成，適用於許多不同的應用，例如報告基因檢測(reporter assays)、SDS-PAGE、蛋白質純化(protein purification)、Western 印跡(Western blot)，免疫沉澱(immunoprecipitation)。Protein yield: 2-8 mg/mL

實驗結果

更高的裂解效率 (More Lysis efficiency)

使用 D 製造商和 Abbkine 的 RIPA 緩衝液裂解分離的 293 細胞懸浮液。將細胞裂解物在 12000 g 下離心 10 分鐘。 1 顯示來自D 製造商的RIPA 裂解緩衝液（弱），2 顯示來自D 製造商的RIPA 裂解緩衝液（中），3 顯示來自D 製造商的RIPA 裂解緩衝液（強），4 顯示來自Abbkine 的RIPA 裂解緩衝液。 D 製造商的 RIPA 緩衝液顯示不完全裂解，透過管底部有明顯的不溶性顆粒證明，而 Abbkine 的 RIPA 裂解緩衝液顯示完全裂解。Isolated 293 cells suspension was lysed by RIPA buffer from D manufacturer and Abbkine. The cell lysates were centrifuged at 12000 g for 10 min. 1 shows RIPA Lysis Buffer(weak) from D manufacturer,2 shows RIPA Lysis Buffer(medium) from D manufacturer, 3 shows RIPA Lysis Buffer(Strong) from D manufacturer, 4 shows RIPA Lysis Buffer from Abbkine. RIPA buffer from D manufacturer show incomplete lysis as evidenced by the presence of obvious insoluble pellet at the bottom of the tube while RIPA Lysis Buffer from Abbkine shows complete lysis.

高蛋白產量(High protein yield) 

採用不同國際品牌、國內知名品牌的蛋白萃取試劑盒產品對小鼠肝組織進行蛋白萃取實驗，包括變性裂解液RIPA和非變性裂解液的處理。結果如下：Different international brands and well-known domestic brands of protein extraction kit products were used to perform protein extraction experiments on mouse liver tissue, including the treatment of denaturated lysate RIPA and non-denaturated lysate. The results are as follows:

In the results of protein extraction by column method of Abbkine, the protein concentration obtained by denatured cleavage was as high as 20.06 mg/mL, and the protein concentration obtained by non-denatured cleavage was 20.99 mg/mL, which ranked top among many products.

No protein loss

Protein profiles of mouse liver extracted by denatureing lysis buffer and native buffer from different manufacturers. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, Marker; Lane 2, native buffer from A manufacturer; Lane 3, native buffer from Abbkine; Lane 4, denatureing lysis buffer from A manufacturer; Lane 5, denatureing lysis buffer from B manufacturer; Lane 6, denatureing lysis buffer from C manufacturer; Lane 7, denatureing lysis buffer from Abbkine; Lane 8, denatureing lysis buffer from D manufacturer.

 

Nuclear protein and membrane protein can be extracted

Total proteins were extracted from 293 cells and mouse liver. Lane 1, 5 μL proteins extracted by assay kits of B manufacturer; Lane 2, 2.5 μL proteins extracted by assay kits of B manufacturer; Lane 3, 5 μL proteins extracted by Abbkine assay kits; Lane 4, 2.5 μL proteins extracted by Abbkine assay kits.

The results showed that the Abbkine kit could extract nuclear protein and membrane protein more particularly effective, and the protein bands with clear background could be obtained in both cells and tissues.