【Yeast Exract酵母提取物 】NEOGEN貨號NCM0218A
6公升迷你培養箱 | 菌液測量 (分光光度計) | 滅菌釜 | 水浴槽WaterBath | 電子天平 | pH酸鹼度計 | Vortex | Pipet aid | Petri dishes 培養皿
酵母提取物(Yeast Exract) 貨號NCM0218A
產品介紹: 酵母提取物(Yeast Extract) 是酵母細胞的自溶產物，用於在實驗室環境中製備微生物培養基。酵母提取物不適用於人類疾病或其他疾病的診斷。酵母提取物(Yeast Extract)是自溶酵母(autolyzed yeast)的水溶性部分(water-soluble)。保留天然存在的B複合維生素(B-complex vitamins)。酵母提取物(Yeast Extract)經製備和標準化後可用於細菌學和細胞培養，是細菌生長的極佳刺激劑。酵母提取物通常以0.3％-0.5％的濃度使用。酵母提取物通常是通過在富含碳水化合物的植物培養基中生長麵包酵母酵母（Saccharomyces spp）來製備的。酵母被收穫，洗滌並重懸於水中，然後在其中進行自溶(autolysis)，即使用酵母酶進行自我消化。酵母提取物是這種自溶作用的總可溶部分(total soluble portion)。自溶活性通過加熱步驟停止。過濾得到的酵母提取物(Yeast Extract)，並通過噴霧乾燥(spray drying)將其乾燥成粉末。酵母提取物已經成功地在培養基中用於牛奶和其他乳製品的細菌研究。已經推薦了幾種含有酵母提取物的培養基用於細胞培養。
References
- www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical manualBAM/default.htm.
- Eaton, A. D., L. S. Clesceri, and A. E. Greenberg (eds.). 2017. Standard methods for the examination of water and wastewater, 23rd ed. American Public Health Association, Washington, D.C.
- Vanderzant, C., and D. F. Splittstoesser (eds.). 2015. Compendium of methods for the microbiological examination of food, 4 th ed. American Public Health Association, Washington, D.C.
- Marshall, R.T. (ed.). 2004. Standard methods for the examination of dairy products, 17th ed. American Public Health Association, Washington, D. C.
- United States Pharmacopeia National Formulary 2018.
- Chan, L., P. F. Greenfield, and S. Reid. 1998. Optimizing fed-batch production of recombinant proteins using the baculovirus expression vector system. Biotechnology BioEngineering, 59: 178-188, John Wiley & Sons, Inc.
- Ikonomou, Bastin, Schneider, Agathose. 2001. Design of efficient medium for insect cell growth and recombinant protein production, in Vitro Cell Dev. Biol. Anim., 37:549-559.
| Product # | Product Description |
| NCM0218A | Yeast Extract, 500 g |
| NCM0218B | Yeast Extract, 5 kg |
| NCM0218C | Yeast Extract, 10 kg |
更多資料來自FDA生菌數之檢驗 (參考方法)
文獻References
- European Pharmacopoeia 9th Edition (2017) 2. United States Pharmacopeia National Formulary 2017: USP 40 NF 35 3. Japanese Pharmacopeia 17th Edition (2017) 4. Sabouraud, R. 1892. Ann. Dermatol. Syphilol. 3:1061. 5. Jarett, L., and A. C. Sonnenwirth (eds.). 1980. Gradwohl’s and parasitic infections, 7th ed. American Public Health Association, Washington, D.C.
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