【ScienCell 品牌】

ScienCell Research Laboratories (ScienCell) 成立於1999年，專長為生產研究用與治療用細胞株及其相關產品。目前已穩定提供各種高品質之正常人類、動物初級細胞(Primary Cells 大鼠巨噬細胞)以供學術及產業界進行研究，另備有多種細胞培養基(Cell culture media)與試劑(Reagents 胰蛋白酶檢測)、細胞生長因子(Growth factors)、細胞來源之DNA/RNA/蛋白質、細胞相關檢測試劑(Cell Based AssaysCK肌酸激酶檢測 ) 與幹細胞&檢測試劑(Stem Cellls 纖維黏連蛋白粘附檢測)、細胞組織染劑 (油紅 O (Oil Red O) )等產品，協助各種細胞治療方法之研究與開發。

ScienCell憑藉尖端技術和嚴格的質量控制標準，為研究人員提供來自 24 個正常人類和動物系統的 260 多種可靠、高質量的初代細胞(primary cells)，包括神經(nervous)、心臟(cardiac)、肝臟(hepatic)、腎臟(renal)、胃腸道(gastrointestinal)、肺(pulmonary)、間充質(mesenchymal)、脂肪(adipose)、口腔(oral)、卵巢(ovarian)、前列腺(prostate)、毛髮(hair)、淋巴(lymphatic)、骨骼(skeletal)、乳腺(mammary)、臍(umbilical)、尿道(urethral)、眼(ocular)等，其中許多是獨有的。

初代細胞(Primary cells)與永生化細胞系(immortalized cell lines)的不同之處在於有限的群體倍增(population doubling)。在沒有遺傳(genetic)和化學修飾(chemical modifications)的情況下，初代細胞(primary cells)保留了其起源組織系統的許多重要生理特性，並密切模擬體內條件。因此，它們為從基礎細胞生物學(cell biology)和生理學(physiology)、發育生物學(developmental biology)、疾病機製(disease mechanisms)到藥物篩选(drug screening)和治療開發(therapeutics development)的各種研究提供了理想的細胞模型。使用 ScienCell 獨有的方法，我們的原代細胞從健康的人類和動物組織中分離出來，在早期傳代時冷凍保存，具有出色的細胞活力。我們還提供定制的細胞分離服務、細胞衍生的 DNA、RNA 和蛋白質。從下面的類別中選擇以查看 ScienCell 提供的原代細胞產品。

Hematopoietic Cell System

The Hematopoietic Cell System includes all blood-derived cells that are produced in the bone marrow by hematopoietic stem and progenitor cells. Hematopoietic cells are grouped into two main categories known as myeloid cells and lymphoid cells. We offer a variety of hematopoietic cells including bone marrow mononuclear cells, splenocytes, lymphatic mononuclear cells, liver mononuclear cells, and macrophages.

造血細胞系統包括由造血幹細胞和祖細胞在骨髓中產生的所有血液來源的細胞。造血細胞分為兩大類，稱為髓樣細胞和淋巴樣細胞。我們提供多種造血細胞，包括骨髓單核細胞、脾細胞、淋巴單核細胞、肝單核細胞和巨噬細胞

CAT. NO.	CODE	DESCRIPTION
1921	MaM	Macrophage Medium
2540	HLyMC	Human Lymphatic Mononuclear Cells
5050	HLMC	Human Liver Mononuclear Cells
5340	HHMa	Human Hepatic Macrophages
5501	HemGM	HematoGro Medium
5521	HemGM-ACF	HematoGro Medium–Animal Component Free
m1920	MMa-bm	Mouse Macrophages from CD1
m1920	MMa-bm	Mouse Macrophages from C57BL/6
m1930	MBMMC	Mouse Bone Marrow Mononuclear Cells from CD1
m1930	MBMMC	Mouse Bone Marrow Mononuclear Cells from C57BL/6
m2540	MLMC	Mouse Lymphatic Mononuclear Cells from CD1
m2540-57	MLMC	Mouse Lymphatic Mononuclear Cells from C57BL/6
m5340	MHMa	Mouse Hepatic Macrophages from CD1
m5340	MHMa	Mouse Hepatic Macrophages from C57BL/6
m5540	MS	Mouse Splenocytes from CD1
m5540-57	MS	Mouse Splenocytes from C57BL/6
m5550	MSMa	Mouse Splenic Macrophages from CD1
m5550-57	MSMa	Mouse Splenic Macrophages from C57BL/6
r1920	RMa-bm	Rat Macrophages
r2540	RLMC	Rat Lymphatic Mononuclear Cells
r5340	RHMa	Rat Hepatic Macrophages
r5540	RS	Rat Splenocytes
r5550	RSMa	Rat Splenic Macrophages

油紅 O (Oil Red O)  | Safranin O Staining Kit SCIENCELL貨號8348

油紅 O (Oil Red O)是一種脂溶性重氮染料(fat-soluble diazo dye)，用於染色細胞和組織中的脂質 (lipid)和脂肪沉積物(fat deposits)。雖然樣品可能是新鮮的、冷凍的或福爾馬林(formalin)固定的，但 Oil Red O 與石蠟包埋的組織切片(paraffin embedded tissue sections)不兼容。觀察到油紅 O 沉澱(precipitates)是正常的。因此，必須使用 Whatman 濾紙或註射器驅動的過濾裝置通過過濾來新鮮製備工作溶液。

Catalog No.	8348
Country of Manufacture	United States
Product Code	SafraninO
Size/Quantity	100 reactions
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Room temperature
Shipping Info	Ambient temperature.
References	[[1] Mackay, A. M., Beck, S. C., Murphy, J. M., Barry, F. P., Chichester, C. O., & Pittenger, M. F. (1998). Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Engineer. 4:415-428.

茜素紅 S(Alizarin Red S)  | Alizarin Red S Staining Kit SCIENCELL貨號0223

茜素紅 S(Alizarin Red S) 是一種蒽醌染料(anthraquinone dye)，用於染色鈣沉積物(calcium deposits)，鈣沉積物是成熟骨細胞(mature osteocytes)的指標。該試劑盒包含 100mL 2% 茜素紅 S 染色劑(Alizarin Red S Stain)，採用方便的即用型溶液(ready-to-use solution)。染料在螯合(chelation)過程中與鈣形成絡合物，從而產生雙折射 (birefringence) [1]。

Materials Supplied by User

• 4% Paraformaldehyde solution in Phosphate Buffered Saline (PBS)
• Deionized H₂O (diH₂O)

Catalog No.	0223
Country of Manufacture	United States
Product Code	ARed
Size/Quantity	100 ml
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Room Temperature
Shipping Info	Ambient temperature.
References	[1 ] Clark, G. (Ed.).(1981). Staining Procedures. (4th ed.) Baltimore, MD: Williams & Wilkins.

CAT. NO.	CODE	DESCRIPTION
0223	ARed	Alizarin Red S Staining Kit
0883	HGL-qPCR	Human Germ Layer Detection qPCR Kit
0843	ORed	Oil Red O Staining Kit
0853	HSC-qPCR	Human Stem Cell Pluripotency Detection qPCR Kit
8048	HMSC-O-qPCR	Human Mesenchymal Stem Cell Osteogenesis Detection qPCR Kit
8258	ALP	Alkaline Phosphatase Activity Assay
8288	ALPr	Alkaline Phosphatase Staining Assay (Red)
8298	HMSC-A-qPCR	Human Mesenchymal Stem Cell Adipogenesis Detection qPCR Kit
8238	HMSC-C-qPCR	Human Mesenchymal Stem Cell Chondrogenesis Detection qPCR Kit
8348	SafraninO	Safranin O Staining Kit
8738	ABlue	Alcian Blue Staining Kit
8678	ARed-Q	Alizarin Red S Staining Quantification Assay

胰蛋白酶檢測 | Colorimetric Trypsin Activity Assay SCIENCELL貨號8898

胰蛋白酶(Trypsin)是絲氨酸蛋白酶家族(serine protease family)的成員。它通過水解賴氨酸(lysine)和精氨酸(arginine)殘基羧基側(residues carboxyl side)的肽鍵將蛋白質和肽切割成更小的片段。胰蛋白酶(Trypsin)作為無活性的胰蛋白酶原(trypsinogen)由胰腺產生，然後分泌到小腸(small intestine)中，在小腸中被腸肽酶(enteropeptidase)裂解並被激活。胰蛋白酶活性異常與胃腸道疾病有關，例如胰腺炎(pancreatitis)和腸黏膜病變(intestinal mucosal pathology)。 ScienCell 的比色胰蛋白酶活性檢測試劑盒 (Trypsin Activity Assay Kit , TRYP) 提供了一種快速靈敏的方法來確定哺乳動物細胞/組織裂解物、血清、血漿和其他生物體液樣品中的胰蛋白酶活性。簡而言之，胰蛋白酶裂解底物並釋放對硝基苯胺 (p-nitroanilide , pNA)，這是一種可以使用分光光度計在 405 nm 處測量的髮色團。胰凝乳蛋白酶是另一種結構相似的絲氨酸蛋白酶(Chymotrypsin)，它不會干擾該測定，因為它不會切割胰蛋白酶底物(trypsin substrate)以釋放 pNA。

Catalog No.	8898
Country of Manufacture	United States
Product Code	TRYP
Size/Quantity	100 Tests in 96-well plate
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store in -20°C
Shipping Info	Dry ice.

CK肌酸激酶檢測 | Creatine Kinase Assay SCIENCELL貨號8618

肌酸激酶 (Creatine kinase ,CK) 通過 ATP 催化肌酸(creatine)的可逆磷酸化(reversible phosphorylation)，形成磷酸肌酸(phosphocreatine)和 ADP。 肌酸激酶 (Creatine kinase ,CK) 由兩個亞基組成：M（muscle, 肌肉）和 B（brain,腦）亞基，並具有三種同工酶：CK-MM（存在於骨骼肌中）、CK-MB（存在於心肌中）和 CK-BB（存在於在大腦和肺中）。臨床上，血液中的 CK 水平用於檢測和/或監測心肌梗塞myocardial infraction（心臟病發作）、橫紋肌溶解(rhabdomyolysis)（嚴重的肌肉衰竭）、肌營養不良(muscular dystrophy)和急性腎功能衰竭(acute renal failure)，因為這些疾病常見 CK 活性水平升高.在這種比色測定中，CK 將磷酸肌酸和 ADP 轉化為肌酸和 ATP，然後 ATP 用於隨後的酶偶聯反應以形成 NADPH。 CK 活性是通過測定 NADPH 形成的速率，速率與 340nm 處的吸光度隨時間的減少 (ΔOD340nm/min) 成正比。

Catalog No.	8618
Country of Manufacture	United States
Product Code	CK
Size/Quantity	100 Tests
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store in -20°C
Shipping Info	Dry ice.

CAT. NO.	CODE	DESCRIPTION
8068	GalC	Beta-Galactosidase Colorimetric Assay
8078	LDH	LDH Cytotoxicity Assay
8108	pNPP	pNPP Phosphatase Assay
8148	GAPDH	Colorimetric GAPDH Assay
8198	SOD	Superoxide Dismutase Assay
8218	CAT	Catalase Activity Assay
8238	GPx	Glutathione Peroxidase Assay
8278	COX	Cytochrome C Oxidase Assay
8318	CS	Citrate Synthase Assay
8358	GDH	Glutamate Dehydrogenase Assay
8408	HK	Hexokinase Assay
8428	G6PDH	Glucose-6-phosphate Dehydrogenase Assay
8458	XO	Xanthine Oxidase Assay
8478	ALT	Alanine Transaminase Assay
8508	GPDH	Glycerol-3-phosphate Dehydrogenase Assay
8548	GST	Glutathione S-transferases Assay
8568	ADH	Alcohol Dehydrogenase Assay
8578	ALDH	Aldehyde Dehydrogenase Assay
8598	PLD	Phospholipase D Assay
8608	AST	Aspartate Transaminase Assay
8618	CK	Creatine Kinase Assay
8628	PK	Pyruvate Kinase Assay
8638	MDH	Malate Dehydrogenase Assay
8658	PDH	Pyruvate dehydrogenase Assay
8668	HAT	Colorimetric Histone Acetyltransferase Activity Assay
8888	HDAC	Colorimetric Histone Deacetylase Activity Assay
8898	TRYP	Colorimetric Trypsin Activity Assay

纖維黏連蛋白粘附檢測 | Fibronectin Cell Adhesion Assay貨號8018

細胞粘附(Cell adhesion)在細胞通訊(cellular communication)和調節(regulation)中起重要作用，對組織的發育(development)和維持(maintenance)具有重要意義。纖維黏連蛋白(fibronectin)是一種高分子量的細胞外基質醣蛋白(extracellular matrix glycoprotein)，參與許多細胞過程，包括組織修復(tissue repair)、胚胎形成 (embryogenesis)、血液凝固(blood clotting)和細胞遷移(cell migration )/粘附(adhesion)。 ScienCell^TM 纖維黏連蛋白粘附檢測(Fibronectin Cell Adhesion Assay)專為快速、定量和可靠地測量細胞對纖維黏連蛋白(fibronectin)的粘附(cell adhesion)而設計。該試劑盒由預塗有纖維黏連蛋白(fibronectin)的 48 孔板以及作為陰性對照的 BSA 組成（參見圖 1 中的板格式）。細胞在預包被的孔中培養一段所需的時間，然後洗去未結合的細胞，固定和染色粘附的細胞，然後進行提取步驟，將染色細胞中的染料洗脫到上清液中。因此，可以使用比色 ELISA 酶標儀在 595 nm 處量化細胞粘附。

Catalog No.	8018
Country of Manufacture	United States
Product Code	Fibro
Size/Quantity	48 assays
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Medium 4°C
Shipping Info	Gel ice.

 

CAT. NO.	CODE	DESCRIPTION
8008	Col	Collagen I Cell Adhesion Assay
8018	Fibro	Fibronectin Cell Adhesion Assay
8028	MT10	MTT Cell Viability & Proliferation Assay
8038	WST	WST-1 Cell Viability & Proliferation Assay
8058	CSA	Cell Senescence Assay
8078	LDH	LDH Cytotoxicity Assay
8088	TUNEL	Colorimetric TUNEL Apoptosis Assay
8138	CSK	Live/Dead Cell Staining Kit
8158	IVTFA	In Vitro Tube Formation Assay
8178	C3DGK	Collagen I-3D Gelling Kit
8188	CCCSCK	Collagen I-Cell Culture Surface Coating Kit
8208	MYCO	Mycoplasma PCR Detection Kit
8228	CAS	Caspase-3 Assay
8808	3D-GSP	3D Gel Staining Prep Kit
8988	HLCC	HeLa Cell Contamination Detection Kit
GK801	AHKRQ	Absolute Human Kringle Repeats Quantification qPCR Assay Kit
GK820	HSDQ	GeneQuery™ Human Biological Sex Determination qPCR Assay Kit (HSDQ)

 

GSH/GSSG檢測套組 | GSH/GSSG Ratio AssayCatalog 貨號8558

穀胱甘肽(Glutathione) 是一種三肽（g-glutamylcysteinylglycine , g-谷氨酰半胱氨酰甘氨酸），以還原（reduced GSH）和氧化（GSSG）狀態存在。穀胱甘肽過氧化物酶催化由 GSH 形成 GSSG，穀胱甘肽還原酶將 GSSG 再循環為 GSH。隨著氧化應激(oxidative stress)水平的增加，細胞內 GSSG 會積累，GSH/GSSG 比率會降低。因此，測定 GSH/GSSG 比值是細胞和組織中氧化應激的有用指標。這種比色測定基於 5′, 5′-二硫代雙 2-硝基苯甲酸 (DTNB) 和 GSH 之間的反應形成 TNB，其在 412 nm 處表現出最大吸光度。吸光度的強度與樣品中的 GSH 水平成正比。GSH/GSSG 比率測定試劑盒可測量不同類型樣品（如組織、細胞裂解物和血漿）中 GSH 和 GSSG 之間的比率。本產品僅用於研究目的，不得用於動物、人類或診斷程序。

Catalog No.	8558
Country of Manufacture	United States
Product Code	GSH/GSSG
Size/Quantity	100 Tests
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store in -20°C
Shipping Info	Dry ice.

 

CAT. NO.	CODE	DESCRIPTION
8008	Col	Collagen I Cell Adhesion Assay
8018	Fibro	Fibronectin Cell Adhesion Assay
8028	MT10	MTT Cell Viability & Proliferation Assay
8038	WST	WST-1 Cell Viability & Proliferation Assay
8058	CSA	Cell Senescence Assay
8078	LDH	LDH Cytotoxicity Assay
8088	TUNEL	Colorimetric TUNEL Apoptosis Assay
8138	CSK	Live/Dead Cell Staining Kit
8158	IVTFA	In Vitro Tube Formation Assay
8178	C3DGK	Collagen I-3D Gelling Kit
8188	CCCSCK	Collagen I-Cell Culture Surface Coating Kit
8208	MYCO	Mycoplasma PCR Detection Kit
8228	CAS	Caspase-3 Assay
8808	3D-GSP	3D Gel Staining Prep Kit
8988	HLCC	HeLa Cell Contamination Detection Kit
GK801	AHKRQ	Absolute Human Kringle Repeats Quantification qPCR Assay Kit
GK820	HSDQ	GeneQuery™ Human Biological Sex Determination qPCR Assay Kit (HSDQ)

 

CAT. NO.	CODE	DESCRIPTION
8118	MGPA	Malachite Green Phosphate Assay
8128	CCA	Colorimetric Calcium Assay
8308	LAC	L-Lactate Assay
8338	GLU	Glutamate Assay
8368	NAD	NAD/NADH Assay
8388	PYR	Pyruvate Assay
8398	G6P	Glucose-6-phosphate Assay
8418	GL	Glucose Assay
8438	US	Uric Acid Assay
8448	IR	Iron Assay
8468	GLY	Glycerol Assay
8498	TG	Triglyceride Assay
8518	TGA	Total Glutathione Assay
8528	AA	Ammonia Assay
8558	GSH/GSSG	GSH/GSSG Ratio Assay
8588	Cho	Choline Assay
8648	Mal	Malate Assay